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Further characterization of tomato-infecting begomoviruses in Brazil

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Abstract

Tomato cultivation in Brazil is threatened by a number of tomato-infecting viruses belonging to the genus Begomovirus of the family Geminiviridae. Here, we report the full DNA-A sequences of three Brazilian begomoviruses: a potentially new tomato-infecting viruses, tomato interveinal chlorosis virus (ToICV), and two previously proposed begomoviruses for which only partial DNA-A sequences are available in the databases: tomato mottle leaf curl virus (TMoLCV) and tomato golden vein virus (TGVV). The complete sequences of the DNA-B components of TMoLCV and TGVV and the DNA-A components of a number of tomato severe rugose virus variants are also presented. Collectively, all of the analyzed sequences were phylogenetically clustered within the two major groups of Brazilian tomato-infecting begomoviruses.

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Acknowledgments

The authors thank Lucio Flávio Barbosa, Oneilson Medeiros de Aquino, Hamilton José Lourenço and Carolina de Mello Franco for technical assistance. AV was supported by the Carnegie Corporation of New York. This study was funded by CNPq and Embrapa. L.C.A. is sponsored by CNPq (561405/2008-5), and A.K.I.N. is a CNPq fellow.

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Authors and Affiliations

  1. Department Fitopatologia, Universidade de Brasília, Brasília, DF, 70910-900, Brazil

    Leonardo C. Albuquerque

  2. Embrapa Vegetables, Km 09, BR060, Cx. Postal 218, Brasília, DF, 70359-970, Brazil

    Fernanda R. Fernandes, Bruna Pinheiro, Paulo de Tarso Oliveira Ferreira, Thaís Oliveira Lemos & Alice K. Inoue-Nagata

  3. School of Biological Science, University of Canterbury, Christchurch, 8140, New Zealand

    Arvind Varsani

  4. Electron Microscope Unit, University of Cape Town, Cape Town, 7701, South Africa

    Arvind Varsani

  5. Biomolecular Interaction Centre, University of Canterbury, Christchurch, 8140, New Zealand

    Arvind Varsani

  6. Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, Anzio Rd, Cape Town, 7925, South Africa

    Darren P. Martin

Authors
  1. Leonardo C. Albuquerque
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  2. Arvind Varsani
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  3. Fernanda R. Fernandes
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  4. Bruna Pinheiro
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  5. Darren P. Martin
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  6. Paulo de Tarso Oliveira Ferreira
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  7. Thaís Oliveira Lemos
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  8. Alice K. Inoue-Nagata
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Corresponding author

Correspondence to Alice K. Inoue-Nagata.

Additional information

The sequences reported in this paper have been deposited in the GenBank under the accession numbers JF803246-JF803265.

Electronic supplementary material

Below is the link to the electronic supplementary material.

705_2011_1213_MOESM1_ESM.docx

Supplementary material 1 (DOCX 42 kb). Supplementary Table S1. Pairwise percentage identities between the complete nucleotide sequences of the DNA-A component of eighteen isolates present in this study and the most closely related viruses. Supplementary Table S2. Nucleotide (nt) and deduced amino acid (aa) sequence identities (%) between BR:PADFM:04 and the most closely related viruses from Brazil. Supplementary Table S3. Nucleotide (nt) and deduced amino acid (aa) sequence identities (%) between BR:Pip1799:03 and the most closely related viruses from Brazil. Supplementary Table S4. Nucleotide (nt) and deduced amino acid (aa) sequence identities (%) between BR:BSF2729:04 and the most closely related viruses from Brazil

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Albuquerque, L.C., Varsani, A., Fernandes, F.R. et al. Further characterization of tomato-infecting begomoviruses in Brazil. Arch Virol 157, 747–752 (2012). https://doi.org/10.1007/s00705-011-1213-7

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  • DOI: https://doi.org/10.1007/s00705-011-1213-7

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