Abstract
The complete genome sequence of a cucurbit-infecting fabavirus was determined. Sequence analysis revealed that it had a genomic organization typical of fabaviruses, with genome segment sizes of 5870 nt (RNA-1) and 3294 nt (RNA-2). It shared CP and Pro-Pol amino acid sequence identities of 52.0–58.9% with those of reported fabaviruses. ELISA and western blots gave no cross-reactions between this cucurbit virus and broad bean wilt viruses 1 and 2. Based on molecular and serological criteria for species demarcation in the genus Fabavirus, the virus represents a distinct species, for which the species name Cucurbit mild mosaic virus (CuMMV) is proposed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Avgelis A, Vovlas C (1989) Artichoke yellow ringspot nepovirus naturally infecting cucumber in Crete. Eur J Plant Pathol 95:177–184
Gu Q, Fan Z, Li H (2002) List of cucurbits virus name. China Cucurbits Veg 1:45–47
Kobayashi Y, Kobayashi A, Nakano M, Hagiwara K, Honda Y, Omura T (2003) Analysis of genetic relations between Broad bean wilt virus 1 and Broad bean wilt virus 2. J Gen Plant Pathol 69:320–326
Kobayashi Y, Kobayashi A, Hagiwara K, Uga H, Mikoshiba Y, Naito T, Honda Y, Omura T (2005) Gentian mosaic virus: a new species in the genus Fabavirus. Phytopathology 95:192–197
Koh L, Cooper J, Wong S (2001) Complete sequences and phylogenetic analyses of a Singapore isolate of Broad bean wilt fabavirus. Arch Virol 146:135–147
Kumar S, Nei M, Dudley J, Tamura K (2008) MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform 9:299–306
Qi Y, Zhou X, Yu Y, Xue C, Li D (1999) Processing sites of polyprotein encoded by RNA-2 of Broad bean wilt virus. Acta Phytopathol Sin 29:280–282
Sanfacon H, Iwanami T, Karasev AV, van der Vlugt R, Wellink J, Wetzel T, Yoshikawa N (2011) Secoviridae. In: King AMQ, Adams MJ, Carstens EB, Lefkowitz EJ (eds) Virus taxonomy, ninth report of the international committee on the taxonomy of viruses. Elsevier Academic Press, London, pp 881–899
Tsuda S, Usugi T, Tomitaka Y, Yasuda F, Okayama H (2010) A novel nepovirus isolated from Cucumis melo in Japan. Phytopathology 101:316–322
Wang R, Ding L, Sun Q, Li J, Xu Z, Peng S (2008) Genome sequence and characterization of a new virus infecting Mikania micrantha HBK. Arch Virol 153:1765–1770
Zhou X, Li D (1996) Purification of broad bean wilt virus and analysis of viral proteins and RNAs. Chin J Virol 12:364–370
Zhou X, Qi Y, Li F, Huang X, Li D (2001) Complete nucleotide sequence and possible genomic expression strategy of a Chinese isolate of Broad bean wilt virus. Acta Biochim Biophys Sin 33:44–52
Acknowledgments
This work was supported in part by the Program for Changjiang Scholars and Innovative Research Team in University (IRT1042) and the National Natural Science Foundation of China (31000840). We thank Professor Xueping Zhou from Zhejiang University for supplying us with BBWV-2 monoclonal antibody and plant samples infected by BBWV-2, and we thank Editor H. Josef Vetten for useful comments and English revisions in the manuscript.
Author information
Authors and Affiliations
Corresponding author
Additional information
S.-W. Dong and H.-Y. Xiang contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Dong, SW., Xiang, HY., Shang, QX. et al. Complete genomic sequence analysis reveals a novel fabavirus infecting cucurbits in China. Arch Virol 157, 597–600 (2012). https://doi.org/10.1007/s00705-011-1202-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-011-1202-x