Abstract
Due to the need to track and monitor genetic diversity, the genome of the infectious hypodermal and hematopoietic necrosis virus (IHHNV) strain KLV-2010-01 in cultured Litopenaeus vannamei shrimp that originated from the first Korean outbreak in 2010 was sequenced and analyzed. The genome, with a length of 3914 nucleotides, was sequenced from the Korean IHHNV. The genome encoded three large and overlapping open reading frames: ORF1 (NS-1) of 2001 bp, ORF2 (NS-2) of 1092 bp and ORF3 (capsid protein) of 990 bp. The overall organization, size and predicted amino acid sequence of the three ORFs in Korean IHHNV were highly similar to those of members of the infectious IHHNV group, and the most closely related strains were IHHNVs described from Ecuador and Hawaii. Additionally, phylogenetic analysis showed that the Korean IHHNV was clustered with lineage III in the infectious IHHNV group and was most similar to IHHNV isolates from Ecuador, China and Taiwan.
Similar content being viewed by others
References
Drummond A, Rambaut A (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7:214
Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy. Eighth report of the international committee on taxonomy of viruses. Elsevier, Amsterdam
Hsieh CY, Chuang PC, Chen LC, Chien T, Chien MS, Huang KC, Kao HF, Tung MC, Tsai SS (2006) Infectious hypodermal and haematopoietic necrosis virus (IHHNV) infections in giant freshwater prawn, Macrobrachium rosenbergii. Aquaculture 258:73–79
Kim JH, Choresca CH, Shin SP, Han JE, Jun JW, Han SY, Park SC (2011) Detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in Litopenaeus vannamei shrimp cultured in South Korea. Aquaculture 313:161–164
Lightner DV, Redman RM, Bell TA (1983) Infectious hypodermal and hematopoietic necrosis, a newly recognized virus disease of penaeid shrimp. J Invertebr Pathol 42:62–70
Lightner DV, Redman RM (1998) Shrimp diseases and current diagnostic methods. Aquaculture 164:201–220
Mari J, Bonami JR, Lightner D (1993) Partial cloning of the genome of infectious hypodermal and haematopoietic necrosis virus, an unusual parvovirus pathogenic for penaeid shrimps; diagnosis of the disease using a specific probe. J Gen Virol 74:2637–2643
OIE (2010) Aquatic animal health code, 11th edn. World Organization for Animal Health, Paris
Pérez-Losada M, Porter ML, Tazi L, Crandall KA (2007) New methods for inferring population dynamics from microbial sequences. Infect Genet Evol 7:24–43
Posada D (2008) jModelTest: phylogenetic model averaging. Mol Biol Evol 25:1253–1256
Rai P, Safeena MP, Karunasagar I, Karunasagar I (2011) Complete nucleic acid sequence of Penaeus stylirostris densovirus (PstDNV) from India. Virus Res 158:37–45
Robles-Sikisaka R, Bohonak AJ, McClenaghan LR Jr, Dhar AK (2010) Genetic signature of rapid IHHNV (Infectious hypodermal and hematopoietic necrosis virus) expansion in wild Penaeus shrimp populations. PLoS One 5:e11799
Saksmerprome V, Puiprom O, Noonin C, Flegel TW (2010) Detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) in farmed Australian Penaeus monodon by PCR analysis and DNA sequencing. Aquaculture 298:190–193
Acknowledgments
This study was financially supported by the Ministry of Education, Science and Technology and by a Korea Research Foundation grant (KRF-2008-331-E00385).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, J.H., Kim, H.K., Nguyen, V.G. et al. Genomic sequence of infectious hypodermal and hematopoietic necrosis virus (IHHNV) KLV-2010-01 originating from the first Korean outbreak in cultured Litopenaeus vannamei . Arch Virol 157, 369–373 (2012). https://doi.org/10.1007/s00705-011-1155-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00705-011-1155-0