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Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air

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Abstract

Avian influenza virus (AIV) has caused serious epidemics all over the world. Notably, the low-pathogenic AIV H9N2 has been spreading widely, leading to enormous economic losses to the poultry industry. To rapidly monitor airborne H9 AIVs in chicken houses, a real-time RT-PCR method was established and used to detect virus in air samples, and it was also compared with the traditional RT-PCR. The results showed that the real-time RT-PCR possessed high specificity and sensitivity for H9 AIVs, and the sensitivity reached 100 copies/reaction, much higher than the traditional RT-PCR; airborne H9 AIVs were found in the six chicken houses by real-time RT-PCR, and their mean concentrations ranged from 1.25×104 to 6.92×104 copies/m3 air. Overall, the real-time PCR is a valuable tool for detecting airborne H9 AIVs.

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Acknowledgments

This study was sponsored by the following foundation programs: National Natural Science Foundation of China (30871865); Chinese International Cooperation Program (2009DFA32890).

Conflict of interest

No conflict of interest exits in the submission of this manuscript, and the manuscript was approved by all authors for publication.

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Street 61, Tai’an, 271018, Shandong, China

    Jing Lv, Baozhi Wei, Tongjie Chai, Zengmin Miao, Meiling Yao & Rong Huang

  2. Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province, Tai’an, Shandong, China

    Jing Lv, Baozhi Wei, Tongjie Chai, Zengmin Miao, Meiling Yao & Rong Huang

  3. Key Laboratory of Animal Biotechnology and Disease Control and Prevention of Shandong Province, Tai’an, Shandong, China

    Jing Lv, Baozhi Wei, Tongjie Chai, Zengmin Miao, Meiling Yao & Rong Huang

  4. Institute of Military Veterinary PLA, Changchun, China

    Xianzhu Xia & Yuwei Gao

  5. Center for Disease Control and Prevention of Tai’an City, Tai’an, China

    Huili Yang

  6. Institute of Animal Hygiene and Environment Health, Free University Berlin, Berlin, Germany

    Uwe Roesler

Authors
  1. Jing Lv
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  2. Baozhi Wei
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  3. Tongjie Chai
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  4. Xianzhu Xia
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  5. Zengmin Miao
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  7. Yuwei Gao
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  8. Rong Huang
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  9. Huili Yang
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  10. Uwe Roesler
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Correspondence to Tongjie Chai.

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Lv, J., Wei, B., Chai, T. et al. Development of a real-time RT-PCR method for rapid detection of H9 avian influenza virus in the air. Arch Virol 156, 1795–1801 (2011). https://doi.org/10.1007/s00705-011-1054-4

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  • DOI: https://doi.org/10.1007/s00705-011-1054-4

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