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Identification of three immediate-early genes of white spot syndrome virus

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Abstract

Viral immediate-early (IE) genes generally encode regulatory proteins that are critical for viral replication. Their transcription, which is independent of de novo viral protein synthesis, is driven directly by host transcription factors. In this study, we examined promoter activities of 12 predicted regulatory genes of white spot syndrome virus (WSSV) belonging to the zinc finger protein family by EGFP-reporter assays in High Five cells. The results showed that the promoters of three genes (wsv056, wsv403 and wsv465) could drive reporter gene expression, and RT-PCR analysis revealed that their expression in WSSV-infected primary crayfish hemocytes was insensitive to the protein synthesis inhibitor cycloheximide (CHX). Therefore, they are IE genes of WSSV.

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Fig. 1
Fig. 2

Abbreviations

WSSV:

White spot syndrome virus

IE:

Immediate-early

CHX:

Cycloheximide

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Acknowledgments

This work was supported by Natural Science Foundation of China (40776096 and 40976100), National Department Public Benefit Research Foundation (200803012 and 201103034), Fujian Science and Technology Project (2009J01176) and the Earmarked Fund for Modern Agro-industry Technology Research System (No. CARS-47).

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Authors and Affiliations

  1. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration (SOA), Xiamen, 361005, People’s Republic of China

    Fanyu Lin, He Huang, Limei Xu, Fang Li & Feng Yang

  2. School of Life Science, Xiamen University, Xiamen, 361005, People’s Republic of China

    He Huang

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  1. Fanyu Lin
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  2. He Huang
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  3. Limei Xu
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  4. Fang Li
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  5. Feng Yang
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Correspondence to Fang Li or Feng Yang.

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Lin, F., Huang, H., Xu, L. et al. Identification of three immediate-early genes of white spot syndrome virus. Arch Virol 156, 1611–1614 (2011). https://doi.org/10.1007/s00705-011-1004-1

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  • DOI: https://doi.org/10.1007/s00705-011-1004-1

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