Skip to main content
SpringerLink
Log in

Detection and molecular characterisation of rotavirus and norovirus infections in Jordanian children with acute gastroenteritis

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Rotavirus and norovirus are globally important causes of paediatric gastroenteritis, but no studies of viral genotypes have been reported from Jordan. We undertook a molecular epidemiological study in children hospitalised with acute gastroenteritis in Jordan between January 2006 and December 2007. Among 368 children, rotavirus and norovirus infections were detected in 49.5% and 11.4% of children, respectively. Rotavirus genotypes P[8],G1 (56%), P[4],G2 (14%) and P[8],G9 (13%) were most commonly identified, consistent with results of global rotavirus surveillance studies. Norovirus GII.3 was the most commonly detected genotype, followed by GII.4, contrasting with most studies in which GII.4 has predominated.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  1. Amar CF, East CL, Gray J, Iturriza-Gomara M, Maclure EA, McLauchlin J (2007) Detection by PCR of eight groups of enteric pathogens in 4, 627 faecal samples: re-examination of the English case-control Infectious Intestinal Disease Study (1993–1996). Eur J Clin Microbiol Infect Dis 26:311–323

    Article  PubMed  CAS  Google Scholar 

  2. Atmar RL, Opekun AR, Gilger MA, Estes MK, Crawford SE, Neill FH, Graham DY (2008) Norwalk virus shedding after experimental human infection. Emerg Infect Dis 14:1553–1557

    Article  PubMed  Google Scholar 

  3. Chhabra P, Dhongade RK, Kalrao VR, Bavdekar AR, Chitambar SD (2009) Epidemiological, clinical, and molecular features of norovirus infections in western India. J Med Virol 81:922–932

    Article  PubMed  CAS  Google Scholar 

  4. Cunliffe NA, Booth JA, Elliot C, Lowe SJ, Sopwith W, Kitchin N, Nakagomi O, Nakagomi T, Hart CA, Regan M (2010) Healthcare-associated viral gastroenteritis among children in a large pediatric hospital, United Kingdom. Emerg Infect Dis 16:55–62

    Article  PubMed  Google Scholar 

  5. Das BK, Gentsch JR, Cicirello HG, Woods PA, Gupta A, Ramachandran M, Kumar R, Bhan MK, Glass RI (1994) Characterization of rotavirus strains from newborns in New Delhi, India. J Clin Microbiol 32:1820–1822

    PubMed  CAS  Google Scholar 

  6. Gentsch JR, Glass RI, Woods P, Gouvea V, Gorziglia M, Flores J, Das BK, Bhan MK (1992) Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30:1365–1373

    PubMed  CAS  Google Scholar 

  7. King CK, Glass R, Bresee JS, Duggan C (2003) Managing acute gastroenteritis among children: oral rehydration, maintenance, and nutritional therapy. MMWR Recomm Rep 52:1–16

    PubMed  Google Scholar 

  8. Kirby A, Dove W, Ashton L, Hopkins M, Cunliffe NA (2010) Detection of norovirus in mouthwash samples from patients with acute gastroenteritis. J Clin Virol 48:285–287

    Article  PubMed  Google Scholar 

  9. Liu J, Kibiki G, Maro V, Maro A, Kumburu H, Swai N, Taniuchi M, Gratz J, Toney D, Kang G, Houpt E (2011) Multiplex reverse transcription PCR Luminex assay for detection and quantitation of viral agents of gastroenteritis. J Clin Virol 50:308–313

    Article  PubMed  CAS  Google Scholar 

  10. Matthijnssens J, Bilcke J, Ciarlet M, Martella V, Banyai K, Rahman M, Zeller M, Beutels P, Van Damme P, Van Ranst M (2009) Rotavirus disease and vaccination: impact on genotype diversity. Futur Microbiol 4:1303–1316

    Article  CAS  Google Scholar 

  11. Meqdam MM, Youssef MT, Nimri LF, Shurman AA, Rawashdeh MO, al-Khdour MS (1997) Viral gastroenteritis among young children in northern Jordan. J Trop Pediatr 43:349–352

    Article  PubMed  CAS  Google Scholar 

  12. Parashar UD, Burton A, Lanata C, Boschi-Pinto C, Shibuya K, Steele D, Birmingham M, Glass RI (2009) Global mortality associated with rotavirus disease among children in 2004. J Infect Dis 200(Suppl 1):S9–S15

    Article  PubMed  Google Scholar 

  13. Patel MM, Widdowson MA, Glass RI, Akazawa K, Vinje J, Parashar UD (2008) Systematic literature review of role of noroviruses in sporadic gastroenteritis. Emerg Infect Dis 14:1224–1231

    Article  PubMed  Google Scholar 

  14. Patel MM, Hall AJ, Vinje J, Parashar UD (2009) Noroviruses: a comprehensive review. J Clin Virol 44:1–8

    Article  PubMed  CAS  Google Scholar 

  15. Tate JE, Patel MM, Steele AD, Gentsch JR, Payne DC, Cortese MM, Nakagomi O, Cunliffe NA, Jiang B, Neuzil KM, de Oliveira LH, Glass RI, Parashar UD (2010) Global impact of rotavirus vaccines. Expert Rev Vaccines 9:395–407

    Article  PubMed  Google Scholar 

  16. WHO (2009) Meeting of the immunization strategic advisory group of experts, April 2009—conclusions and recommendations. Wkly Epidemiol Rec 84(23):220–236

    Google Scholar 

  17. Youssef M, Shurman A, Bougnoux M, Rawashdeh M, Bretagne S, Strockbine N (2000) Bacterial, viral and parasitic enteric pathogens associated with acute diarrhea in hospitalized children from northern Jordan. FEMS Immunol Med Microbiol 28:257–263

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

ON and TN are honorary members of The University of Liverpool and participated in this study according to the Agreement on Academic Partnership between The University of Liverpool and Nagasaki University.

Conflicts of interest

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Microbiology Department, King Hussein Medical Centre, PO Box 140746, Amman, 11814, Jordan

    Nasser M. Kaplan & Sawsan A. Abd-Eldayem

  2. Department of Clinical Infection, Microbiology & Immunology, Institute of Infection and Global Health, University of Liverpool, 8th Floor, Duncan Building, Daulby Street, Liverpool, L69 3GA, UK

    Andrew Kirby, Winifred Dove & Nigel A. Cunliffe

  3. Division of Molecular Epidemiology, Graduate School of Biomedical Sciences, Global Center of Excellence, Nagasaki University, Nagasaki, Japan

    Toyoko Nakagomi & Osamu Nakagomi

Authors
  1. Nasser M. Kaplan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrew Kirby
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sawsan A. Abd-Eldayem
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Winifred Dove
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Toyoko Nakagomi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Osamu Nakagomi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Nigel A. Cunliffe
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nasser M. Kaplan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kaplan, N.M., Kirby, A., Abd-Eldayem, S.A. et al. Detection and molecular characterisation of rotavirus and norovirus infections in Jordanian children with acute gastroenteritis. Arch Virol 156, 1477–1480 (2011). https://doi.org/10.1007/s00705-011-0996-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-011-0996-x

Keywords

Search

Navigation