Abstract
Eight human papillomavirus (HPV) types including four cutaneous HPV types (HPV-5, HPV-8, HPV-20 and HPV-38) and four mucosal HPV types (HPV-6, HPV-11, HPV-16 and HPV-18) were selected for this miRNA study. Pre-miRNAs were predicted using a computer programme, and the conserved mature miRNAs were compared to currently known miRNAs. Predicted HPV miRNAs related to miR-466, -467 and -669 were common and specific to the mucosal HPV types. Northern blot hybridization confirmed a predicted miRNA in HPV-positive cervical cancer cell lines encoded by mucosal HPVs. HPV-38 was predicted to express an miRNA conserved to human let-7a and the expression of let-7a, in HPV-38-positive non-melanoma skin cancer (NMSC) biopsies was 10-fold higher than those with HPV-positive (for other types except HPV-38) and HPV-negative NMSCs, suggesting that let-7a expression might be related to HPV-38 infection. Potential gene targets of the predicted miRNA that may aid HPV in infection and pathogenesis were also analysed.
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Abbreviations
- miRNA:
-
MicroRNA
- HPV:
-
Human papillomavirus
- siRNA:
-
Short interfering RNA
- NMSC:
-
Non-melanoma skin cancer
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Acknowledgments
The authors have no conflicting financial interests. The authors would like to express their thanks to Dr. Ping Zhang (Diamantina Institute, the University of Queensland) for her assistant in gene target prediction. This study was funded by the NHMRC with a Peter Doherty Fellowship (WG), Early Career Researcher Grant (WG) from the University of Queensland, Brisbane, the Prostate Cancer Foundation in Australia (AA) and the Mazda foundation (AA), Australia.
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Gu, W., An, J., Ye, P. et al. Prediction of conserved microRNAs from skin and mucosal human papillomaviruses. Arch Virol 156, 1161–1171 (2011). https://doi.org/10.1007/s00705-011-0974-3
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DOI: https://doi.org/10.1007/s00705-011-0974-3