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Molecular characterization of a new begomovirus and divergent alphasatellite from tomato in Cameroon

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References

  1. Briddon RW, Bull SE, Amin I, Mansoor S, Bedford ID, Rishi N, Siwatch SS, Zafar MY, Abdel-Salam AM, Markham PG (2004) Diversity of DNA 1: a satellite-like molecule associated with monopartite begomovirus-DNA β complexes. Virology 324:462–474

    Article  PubMed  CAS  Google Scholar 

  2. Bull SE, Briddon RW, Markham PG (2003) Universal primers for the PCR-mediated amplification of DNA 1: a satellite-like molecule associated with begomovirus-DNA β complexes. Mol Biotechnol 23:83–86

    Article  PubMed  CAS  Google Scholar 

  3. Chen LF, Rojas MR, Kon T, Gamby K, Xoconostle-Cazares B, Gilbertson RL (2009) A severe symptom phenotype in tomato in Mali is caused by a reassortant between a novel recombinant begomovirus (Tomato yellow leaf curl Mali virus) and a betasatellite. Mol Plant Pathol 10:415–430

    Article  PubMed  CAS  Google Scholar 

  4. Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Arch Virol 153:783–821

    Article  PubMed  CAS  Google Scholar 

  5. Kon T, Rojas MR, Abdourhamane IK, Gilbertson RL (2009) Roles and interactions of begomoviruses and satellite DNAs associated with okra leaf curl disease in Mali, West Africa. J Gen Virol 90:1001–1013

    Article  PubMed  CAS  Google Scholar 

  6. Leke WN, Ramsell JNE, Njualem DK, Titanji VPK, Legg JP, Fondong VN, Brown JK, Kvarnheden A (2007) FTA technology facilitates detection and identification of begomoviruses from okra plants in Cameroon. Afr Crop Sci Soc Conf Proc 8:655–660

    Google Scholar 

  7. Lett JM, Lefeuvre P, Couston L, Hoareau M, Thierry M, Reynaud B, Martin DP, Varsani A (2009) Complete genomic sequences of Tomato yellow leaf curl Mali virus isolates infecting tomato and pepper from the North Province of Cameroon. Arch Virol 154:535–540

    Article  PubMed  CAS  Google Scholar 

  8. Martin DP, Lemey P, Lott M, Moulton V, Posada D, Lefeuvre P (2010) RDP3: a flexible and fast computer program for analyzing recombination. Bioinformatics 26:2462–2463

    Article  PubMed  CAS  Google Scholar 

  9. Mubin M, Briddon RW, Mansoor S (2009) Complete nucleotide sequence of chilli leaf curl virus and its associated satellites naturally infecting potato in Pakistan. Arch Virol 154:365–368

    Article  PubMed  CAS  Google Scholar 

  10. Osei MK, Akromah R, Shih SL, Lee LM, Green SK (2008) First report and molecular characterization of three distinct begomoviruses associated with tomato leaf curl disease in Ghana. Plant Dis 92:1585–1585

    Google Scholar 

  11. Rojas MR, Hagen C, Lucas WJ, Gilbertson RL (2005) Exploiting chinks in the plant’s armor: evolution and emergence of geminiviruses. Ann Rev Phytopathol 43:361–394

    Article  CAS  Google Scholar 

  12. Shepherd DN, Martin DP, Lefeuvre P, Monjane AL, Owor BE, Rybicki EP, Varsani A (2008) A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue. J Virol Methods 149:97–102

    Article  PubMed  CAS  Google Scholar 

  13. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

    Article  PubMed  CAS  Google Scholar 

  14. Wyatt SD, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86:1288–1293

    Article  Google Scholar 

  15. Zhou YC, Noussourou M, Kon T, Rojas MR, Jiang H, Chen L-F, Gamby K, Foster R, Gilbertson RL (2008) Evidence of local evolution of tomato-infecting begomovirus species in West Africa: characterization of tomato leaf curl Mali virus and tomato leaf crumple virus from Mali. Arch Virol 153:693–706

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This research was jointly supported by several foundations and organizations to which we express our sincere gratitude: International Foundation of Science, Stockholm, Sweden, through a grant to W.N. Leke; Rothamsted International, through the Rothamsted International African Fellows programme; The Swedish Institute; USAID, through the IPM-CRSP Insect-Transmitted Virus Global Themes project; The Royal Physiographic Society in Lund, Sweden. W.N. Leke completed this study as part of his Ph.D. studies. We sincerely thank the anonymous referees for their useful comments and suggestions.

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Leke, W.N., Kvarnheden, A., Ngane, E.B. et al. Molecular characterization of a new begomovirus and divergent alphasatellite from tomato in Cameroon. Arch Virol 156, 925–928 (2011). https://doi.org/10.1007/s00705-011-0957-4

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  • DOI: https://doi.org/10.1007/s00705-011-0957-4

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