Abstract
Taura syndrome virus (TSV) is a major cause of high mortality in Pacific white shrimp (Litopenaeus vannamei, Lv). Previously, silencing of Penaeus monodon Rab7 (PmRab7) by injecting double-stranded RNA corresponding to PmRab7 (dsRNA-PmRab7) prevented white spot syndrome virus or yellow head virus infection. Rab7 is proposed to be involved in intracellular trafficking of the viruses. This study aimed to investigate whether knockdown of Rab7 in L. vannamei by dsRNA-PmRab7 could inhibit replication of TSV. RNA interference (RNAi) technology using dsRNA targeting the LvRab7 gene was used to silence the mRNA expression of LvRab7. The silencing of the LvRab7 gene inhibited TSV replication dramatically when compared to groups receiving dsRNA-GFP or NaCl. This is the first demonstration that dsRNA targeting the endogenous shrimp gene LvRab7 strongly reduces TSV replication. It provides further evidence that LvRab7 is involved in the endosomal trafficking pathway of viruses infecting penaeid shrimp.
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Acknowledgments
The authors thank Asst. Prof. Dr. Witoon Tirasophon for the viral stock, Assoc. Prof. Dr. Albert Ketterman for critical reading of the manuscript, and Ms. Chawewan Chimawai and Ms. Pensri Hongthong for technical assistance. This work is supported by a Thailand Research Fund (TRF), Commission on Higher Education (CHE) and Mahidol University research grant. C.O. is the recipient of a TRF-CHE research career development grant (RMU4980004).
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Ongvarrasopone, C., Saejia, P., Chanasakulniyom, M. et al. Inhibition of Taura syndrome virus replication in Litopenaeus vannamei through silencing the LvRab7 gene using double-stranded RNA. Arch Virol 156, 1117–1123 (2011). https://doi.org/10.1007/s00705-011-0952-9
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DOI: https://doi.org/10.1007/s00705-011-0952-9