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Rescue of Newcastle disease virus from cloned cDNA using an RNA polymerase II promoter

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Abstract

A new system was developed to improve the efficiency and simplify the procedure of recovery of Newcastle disease virus (NDV) from cloned cDNA. A full-length cDNA clone of mesogenic NDV vaccine strain Mukteswar was assembled from five subgenomic cDNA fragments and cloned into a plasmid allowing transcription driven by cellular RNA polymerase II. The full-length viral cDNA was flanked by hammerhead ribozyme (HamRz) and hepatitis delta virus ribozyme (HdvRz) sequences, resulted in the synthesis of antigenomic RNA with exact termini. Without supplying T7 RNA polymerase, infectious NDV could be generated efficiently in some eukaryotic cell lines by simultaneous transcription of antigenomic RNA from the full-length plasmid and expression of NP, P and L proteins from helper plasmids introduced by cotransfection. The efficiency of recovery with the conventional T7 promoter system based on BRS-T7 cells and the cytomegalovirus (CMV) promoter system was compared, and the results demonstrate that the new system facilitates the generation of recombinant NDV and more efficient than the T7 rescue system using BRS-T7.

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References

  1. Alexander DJ (1988) Newcastle disease and other avian paramyxoviruses. In: A laboratory manual for the isolation and identification of avian pathogens, pp 156–163

  2. Seal BS, King DJ, Sellers HS (2000) The avian response to Newcastle disease virus. Dev. Comp. Immunol. 24:257–268

    Article  PubMed  CAS  Google Scholar 

  3. Lamb RA, Kolakofsky D (2001) Paramyxoviridae: the viruses and their replication. In: Fields virology, pp 1305–1340

  4. Steward M, Vipond IB, Millar NS, Emmerson PT (1993) RNA editing in Newcastle disease virus. J Gen Virol 74:2539–2547

    Article  PubMed  CAS  Google Scholar 

  5. Peeters BPH, de Leeuw OS, Koch G, Gielkens ALJ (1999) Rescue of Newcastle disease virus from cloned cDNA: evidence that cleavability of the fusion protein is a major determinant for virulence. J Virol 73:5001–5009

    PubMed  CAS  Google Scholar 

  6. Romer-Oberdorfer A, Mundt E, Mebatsion T, Buchholz UC, Mettenleiter T (1999) Generation of recombinant lentogenic Newcastle disease virus from cDNA. J Gen Virol 80:2987–2995

    PubMed  CAS  Google Scholar 

  7. Mebatsion T, Verstegen S, de Vaan LT, Romer-Oberdorfer A, Schrier CC (2001) A recombinant Newcastle disease virus with low-level V protein expression is immunogenic and lacks pathogenicity for chicken embryos. J Virol 75:420–428

    Article  PubMed  CAS  Google Scholar 

  8. Peeters BPH, de Leeuw OS, Verstegan I, Koch G, Gielkens ALJ (2001) Generation of a recombinant chimeric Newcastle disease virus vaccine that allows serological differentiation between vaccinated and infected animals. Vaccine 19:1616–1627

    Article  PubMed  CAS  Google Scholar 

  9. Krishnamurthy S, Huang Z, Samal SK (2000) Recovery of a virulent strain of Newcastle disease virus from cloned cDNA: expression of a foreign gene results in growth retardation and attenuation. Virology 278:168–182

    Article  PubMed  CAS  Google Scholar 

  10. Huang Z, Krishnamurthy S, Panda A, Samal SK (2001) High-level expression of a foreign gene from the most 3′ proximal locus of a recombinant Newcastle disease virus. J Gen Virol 82:1729–1736

    PubMed  CAS  Google Scholar 

  11. Liu YL, Hu SL, Zhang YM (2007) Generation of a velogenic Newcastle disease virus from cDNA and expression of the green fluorescent protein. Arch Virol 152:1241–1249

    Article  PubMed  CAS  Google Scholar 

  12. Huang ZH, Elankumaran S, Abdul S, Siba Yunus, Samal K (2004) A recombinant Newcastle disease virus (NDV) expressing VP2 protein of infectious bursal disease virus (IBDV) protects against NDV and IBDV. J Gen Virol 78:10054–10063

    CAS  Google Scholar 

  13. Fuerst TR, Niles EG, Studier FW, Moss B (1986) Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase. Proc Natl Acad Sci USA 83:8122–8126

    Article  PubMed  CAS  Google Scholar 

  14. Buchholz UJ, Finke S, Conzelmann KK (1999) Generation of bovine respiratory syncytial virus (BRSV) from cDNA: BRSV NS2 is not essential for virus replication in tissue culture, and the human RSV leader region acts as a functional BRSV genome promoter. J Virol 73:251–259

    PubMed  CAS  Google Scholar 

  15. Schnell MJ, Mebatsion T, Conzelmann KK (1994) Infectious rabies viruses from cloned cDNA. EMBO J 13:4195–4203

    PubMed  CAS  Google Scholar 

  16. Garcin D, Pelet T, Calain P, Roux L, Curran J, Kolakofsky D (1995) A highly recombinogenic system for the recovery of infectious Sendai paramyxovirus from cDNA: Generation of a novel copy-back nondefective interfering virus. EMBO J 14:6087–6094

    PubMed  CAS  Google Scholar 

  17. Collins PL, Hill MG, Camargo E, Grosfeld H, Chanock RM, Murphy BR (1995) Production of infectious human respiratory syncytial virus from cloned cDNA confirms an essential role for the transcription elongation factor from the 5′ proximal open reading frame of the M2 mRNA in gene expression and provides a capability for vaccine development. Proc Natl Acad Sci USA 92:11563–11567

    Article  PubMed  CAS  Google Scholar 

  18. Lawson ND, Stillman EA, Whitt MA, Rose JK (1995) Recombinant vesicular stomatitis virus from DNA. Proc Natl Acad Sci USA 92:4477–4481

    Article  PubMed  CAS  Google Scholar 

  19. Radecke F, Spielhofer P, Schneider H, Kaelin K, Huber M, Dotsch C, Christiansen G, Billeter MA (1995) Rescue of measles viruses from cloned DNA. EMBO J 14:5773–5784

    PubMed  CAS  Google Scholar 

  20. He B, Paterson RG, Ward CD, Lamb RA (1997) Recovery of infectious SV5 from cloned DNA and expression of a foreign gene. Virology 237:249–260

    Article  PubMed  CAS  Google Scholar 

  21. Durbin AP, Hall SL, Siew JW, Whitehead SS, Collins PL, Murphy BR (1997) Recovery of infectious human parainfluenza virus type 3 from cDNA. Virology 235:323–332

    Article  PubMed  CAS  Google Scholar 

  22. Baron MD, Barrett T (1997) Rescue of Rinderpest virus from cloned cDNA. J Virol 71:1265–1271

    PubMed  CAS  Google Scholar 

  23. Clarke DK, Sidhu MS, Johnson JE, Udem SA (2000) Rescue of mumps virus from cDNA. J Virol 74:4831–4838

    Article  PubMed  CAS  Google Scholar 

  24. Volchkov VE, Volchkova VA, Muhlberger E, Kolesnikova LV, Weik M, Dolnik O, Klenk HD (2001) Recovery of infectious Ebola virus from complementary DNA: RNA editing of the GP gene and viral cytotoxicity. Science 291:1965–1969

    Article  PubMed  CAS  Google Scholar 

  25. Fodor E, Devenish L, Engelhardt OG, Palese P, Brownlee GG, Garcia-Sastre A (1999) Rescue of influenza A virus from recombinant DNA. J Virol 73:9679–9682

    PubMed  CAS  Google Scholar 

  26. Kenichi I, Youko S, Ichiro K, Toshio I, Takeo S, Kinjiro M (2003) An improved method for recovering rabies virus from cloned cDNA. J Virol Methods 107:229–236

    Article  Google Scholar 

  27. Martin A, Staeheli P, Schneider U (2006) RNA polymerase II-controlled expression of antigenomic RNA enhances the rescue efficacies of two different members of the Mononegavirales independently of the site of viral genome replication. J Virol 80:5708–5715

    Article  PubMed  CAS  Google Scholar 

  28. Hideyuki Y, Yohei H, Yohei HW, Naohiro O, Takeshi K, Yasutoshi N, Kazuyoshi I, Keizo T (2006) Development of a novel Borna disease virus reverse genetics system using RNA polymerase II promoter and SV40 nuclear import signal. Microbes Infect 8:1522–1529

    Article  Google Scholar 

  29. Le Mercier P, Jacob Y, Tanner K, Tordo N (2002) A novel expression cassette of lyssavirus shows that the distantly related Mokola virus can rescue a defective rabies virus genome. J Virol 76:2024–2027

    Article  PubMed  CAS  Google Scholar 

  30. Proudfoot NJ (1991) Poly(A) signals. Cell 64:671–674

    Article  PubMed  CAS  Google Scholar 

  31. Kovacs GR, Parks CL, Vasilakis N, Udem SA (2003) Enhanced genetic rescue of negative-strand RNA viruses: use of a MVA-T7 RNA polymerase vector and DNA replication inhibitors. J Virol Methods 111:29–36

    Article  PubMed  CAS  Google Scholar 

  32. Hoffman MA, Banerjee AK (1997) An infectious clone of human parainfluenza virus type 3. J Virol 71:4272–4277

    PubMed  CAS  Google Scholar 

  33. Peeters BP, Gruijthuijsen YK, de Leeuw OS, Gielkens AL (2000) Genome replication of Newcastle disease virus: involvement of the rule-of six. Arch Virol 145:1829–1845

    Article  PubMed  CAS  Google Scholar 

  34. Romer-Oberdorfer A, Werner O, Veits J, Mebatsion T, Mettenleiter T (2003) Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J Gen Virol 84:3121–3129

    Article  PubMed  Google Scholar 

  35. De Leeuw OS, Koch G, Hartog L, Ravenshorst N, Peeters BPH (2005) Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin–neuraminidase protein. J Gen Virol 86:1759–1769

    Article  PubMed  Google Scholar 

  36. Zhao H, Peeters BPH (2003) Recombinant Newcastle disease virus as a viral vector: effect of genomic location of foreign gene on gene expression and virus replication. J Gen Virol 84:781–788

    Article  PubMed  CAS  Google Scholar 

  37. Veits J, Wiesner D, Fuchs W, Hoffmann B, Granzow H, Starick E, Mundt E, Schirrmeier H, Mebatsion T, Mettenleiter TC, Romer-Oberdorfer A (2006) Newcastle disease virus expressing H5 hemagglutinin gene protects chickens against Newcastle disease and avian influenza. Proc Natl Acad Sci USA 103:8197–8202

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported by a grant from the Special Social Commonweal Research Programs of the State (No. 2005DIB4J041).

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Authors and Affiliations

  1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Grazing Animal Diseases of Ministry of Agriculture, Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, People’s Republic of China

    Bao-yu Li, Xue-rui Li, Xi Lan, Xiang-pin Yin, Zhi-yong Li, Bin Yang & Ji-xing Liu

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  1. Bao-yu Li
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  2. Xue-rui Li
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  7. Ji-xing Liu
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Correspondence to Ji-xing Liu.

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Li, By., Li, Xr., Lan, X. et al. Rescue of Newcastle disease virus from cloned cDNA using an RNA polymerase II promoter. Arch Virol 156, 979–986 (2011). https://doi.org/10.1007/s00705-011-0932-0

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  • DOI: https://doi.org/10.1007/s00705-011-0932-0

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