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Genomic comparison between attenuated Chinese equine infectious anemia virus vaccine strains and their parental virulent strains

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Abstract

A lentiviral vaccine, live attenuated equine infectious anemia virus (EIAV) vaccine, was developed in the 1970s, and this has made tremendous contributions to the control of equine infectious anemia (EIA) in China. Four key virus strains were generated during the attenuation of the EIAV vaccine: the original Liao-Ning strain (EIAVLN40), a donkey-adapted virulent strain (EIAVDV117), a donkey-leukocyte-attenuated vaccine strain (EIAVDLV121), and a fetal donkey dermal cell (FDD)-adapted vaccine strain (EIAVFDDV13). In this study, we analyzed the proviral genomes of these four EIAV strains and found a series of consensus substitutions among these strains. These mutations provide useful information for understanding the genetic basis of EIAV attenuation. Our results suggest that multiple mutations in a variety of genes in our attenuated EIAV vaccines not only provide a basis for virulence attenuation and induction of protective immunity but also greatly reduce the risk of reversion to virulence.

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Acknowledgments

This study was funded by National Special Fund for Control and Treatment of Major Infectious Diseases (Grant 2008ZX1001-1010 to JZ), National Natural Science Foundation of China (30771994 to JZ and 30901349 to YL).

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Authors and Affiliations

  1. Division of Large Animal Infectious Diseases, Stated key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150001, People’s Republic of China

    Xuefeng Wang, Shuai Wang, Yuezhi Lin, Chenggang Jiang, Jian Ma, Liping Zhao, Xiaoling Lv, Rongxian Shen, Xiangang Kong & Jianhua Zhou

  2. Animal Science and Animal Medicine School, Inner Mongolia Agricultural University, Huhhot, 010018, People’s Republic of China

    Xuefeng Wang & Fenglong Wang

Authors
  1. Xuefeng Wang
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  2. Shuai Wang
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  3. Yuezhi Lin
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  4. Chenggang Jiang
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  5. Jian Ma
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  6. Liping Zhao
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  7. Xiaoling Lv
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  8. Fenglong Wang
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  9. Rongxian Shen
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  10. Xiangang Kong
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  11. Jianhua Zhou
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Correspondence to Jianhua Zhou.

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Wang, X., Wang, S., Lin, Y. et al. Genomic comparison between attenuated Chinese equine infectious anemia virus vaccine strains and their parental virulent strains. Arch Virol 156, 353–357 (2011). https://doi.org/10.1007/s00705-010-0877-8

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  • DOI: https://doi.org/10.1007/s00705-010-0877-8

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