Abstract
Yellow vein disease (YVD) is a major problem in pepper in West Africa. Despite the recent implication of a begomovirus in YVD in Mali and in Burkina Faso, the aetiology of the disease remains unclear. Using symptomatic samples from the main vegetable cultivation regions in Burkina Faso, 10 full-length DNA-A-like begomovirus sequences were obtained, each showing 98% nucleotide identity to pepper yellow vein Mali virus (PepYVMV). The host range was determined after construction of a viral clone for agroinfection. Severe symptoms developed in tomato and Nicotiana benthamiana. By contrast, no symptoms developed in either commercial or local pepper cultivars, demonstrating that the aetiology of YVD is not only associated with the presence of PepYVMV.
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Acknowledgments
This study was supported by the following institutions: International Foundation for Science (IFS) fellowship no. C/4472-1 to F. Tiendrébéogo, AIRES-Sud: a programme from the French Ministry of Foreign and European Affairs implemented by the Institut de Recherche pour le Développement (IRD-DSF), CRSBAN/UFR-SVT (University of Ouagadougou), CIRAD, Conseil Régional de La Réunion, European Union (FEDER) and GIS Centre de recherche et de veille sanitaire sur les maladies émergentes dans l’Océan Indien (N°PRAO/AIRD/CRVOI/08/03). The technical assistance of Mr. Martial Grondin is gratefully appreciated. FT completed this research as part of his PhD Degree.
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Supplementary table:
Matrix of pairwise identity percentages between the complete DNA-A nucleotide sequences of the six isolates of Pepper yellow vein Mali virus from Burkina Faso (in bold) and some closely related begomoviruses. Percentages of identity over the 89% taxonomic threshold for begomovirus are presented in bold (DOC 65 kb)
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Tiendrébéogo, F., Lefeuvre, P., Hoareau, M. et al. Molecular and biological characterization of Pepper yellow vein Mali virus (PepYVMV) isolates associated with pepper yellow vein disease in Burkina Faso. Arch Virol 156, 483–487 (2011). https://doi.org/10.1007/s00705-010-0854-2
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DOI: https://doi.org/10.1007/s00705-010-0854-2