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Nucleotide sequences of four RNA segments of a reovirus isolated from the mud crab Scylla serrata provide evidence that this virus belongs to a new genus in the family Reoviridae

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Abstract

This is the first sequence-based characterization of mud crab (Scylla serrata) reovirus (SsRV), which causes severe disease of cultured mud crabs in southern China. We sequenced and analyzed genome segments S1, S2, S3, and S7, which were 4,327, 2,721, 2,715, and 1,517 nucleotides long, respectively. Conserved motifs were found at the 5’ (AUAAAU) and 3’ (AACGAU) ends of each segment. RNA segments S1, S2, S3, and S7 each contained a single open reading frame (ORF) that encoded predicted proteins of 160, 100, 96, and 46 kDa, respectively. The ORFs of segments S1 and S2 showed distant homologies (< 25%) with cognate genes of other reoviruses, whereas the ORFs of segments S3 and S7 had no homologies with any other viral genes. Based on these observations, we propose that SsRV should be considered a member of a new genus in the family Reoviridae.

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Fig. 1
Fig. 2

Abbreviations

RdRp:

RNA-dependent RNA polymerase

S.serrata :

Scylla serrata

EsRV:

Eriocheir sinensis reovirus

SsRV:

Scylla serrata reovirus

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (no. 30800856) and the Major Scientific and Technical Project of Ningbo Science and Technology Bureau in Zhejiang Province (no. 2007C11002).

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Authors and Affiliations

  1. College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, 315100, Zhejiang Province, China

    Jigang Chen, Juan Xiong, Jifang Yang & Zhijuan Mao

  2. Municipal Key Laboratory of Microorganism and Environmental Engineering, Ningbo, 315100, China

    Jigang Chen, Juan Xiong, Jifang Yang & Zhijuan Mao

  3. College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China

    Juan Xiong & Xiaoxuan Chen

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  1. Jigang Chen
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  2. Juan Xiong
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  5. Xiaoxuan Chen
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Corresponding author

Correspondence to Jigang Chen.

Additional information

The nucleotide sequence data reported in this paper have been submitted to GenBank and have been assigned the accession numbers HM807276-HM807279 for segments S1, S2, S3 and S7, respectively.

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Chen, J., Xiong, J., Yang, J. et al. Nucleotide sequences of four RNA segments of a reovirus isolated from the mud crab Scylla serrata provide evidence that this virus belongs to a new genus in the family Reoviridae . Arch Virol 156, 523–528 (2011). https://doi.org/10.1007/s00705-010-0852-4

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