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Dual infection by cassava begomoviruses in two leguminous species (Fabaceae) in Yangambi, Northeastern Democratic Republic of Congo

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Abstract

A study on cassava mosaic begomoviruses was conducted around Yangambi (DR Congo) by sampling 10 different leguminous species with or without symptoms similar to cassava mosaic disease. DNA was isolated to amplify CMBs using primers targeting AC2 and AC4 genes for virus detection by PCR. The results showed a dual infection by ACMV and EACMV in two weed species, Centrosema pubescens and Pueraria javanica, associated with mosaic symptoms. The DNA-A genome component of ACMV and EACMV from the infested weeds was sequenced. Seven ACMV and four EACMV isolates are reported. The major ACMV strains were closely related to ACMV-NGogo, ACMV-IC and ACMV-UGMld, whereas all EACMV strains were closely related to a Uganda variant, the most prevalent virus. This study shows that whiteflies may transmit CMBs to non-cassava plants under high epidemic pressure.

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Acknowledgments

This study was supported by a Belgian Technical Cooperation (BTC) scholarship, the Applied Microbiology, Phytopathology unit of UCL in Belgium and the Agricultural Institute Faculty of Yangambi (IFA-Yangambi) in the DRC.

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Authors and Affiliations

  1. Earth and Life Institute, Applied Microbiology, Phytopathology, Université catholique de Louvain, Croix du sud 2bte3, 1348, Louvain-la-Neuve, Belgium

    G. Monde & C. Bragard

  2. Faculté des sciences agronomiques, Université catholique de Bukavu, B-285, Bukavu, Democratic Republic of Congo

    J. Walangululu

  3. Plant Virus Collection, DSMZ Deutsche Sammlung von Mikroorganismen und Zellkuturen GmbH, 38104, Braunschweig, Germany

    S. Winter

  4. Institut Facultaire des Sciences Agronomiques de Yangambi, Unité de production et défense des végétaux, B-1232, Kisangani, Democratic Republic of Congo

    G. Monde

Authors
  1. G. Monde
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  2. J. Walangululu
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  3. S. Winter
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  4. C. Bragard
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Correspondence to C. Bragard.

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Monde, G., Walangululu, J., Winter, S. et al. Dual infection by cassava begomoviruses in two leguminous species (Fabaceae) in Yangambi, Northeastern Democratic Republic of Congo. Arch Virol 155, 1865–1869 (2010). https://doi.org/10.1007/s00705-010-0772-3

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