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Genetic variability in the coat protein gene of Potato virus X and the current relationship between phylogenetic placement and resistance groupings

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Abstract

The complete coat protein nucleotide sequences of 11 Potato virus X isolates from Australia and two from Britain were compared to those of 72 others. On phylogenetic analysis, clade I contained all 11 Australian sequences, and sub-clade II-1 contained the two new British sequences. Clade I isolates were from six different continents, but those in sub-clades II-1 and II-2 were only from Europe and the Americas, respectively. Clade I contained isolates in strain groups 1, 3 and 4, and sub-clades II-1 and II-2, isolates in strain groups 2 and 4. Thus, strain group 4 now occurs within both clades.

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References

  1. Jones RAC (1981) The ecology of viruses infecting wild and cultivated potatoes in the Andean region of South America. In: Thresh JM (ed) Pests, pathogens and vegetation. Pitman, London, UK, pp 89–107

    Google Scholar 

  2. Stevenson WR, Loria R, Franc GD, Weingartner DP (eds) (2001) Compendium of potato diseases, 2nd edn. APS Press, St. Paul, Minnesota

    Google Scholar 

  3. Wilson CR, Jones RAC (1995) Occurrence of PVX strain group 1 in seed stocks of potato cultivars lacking resistance genes. Ann Appl Biol 127:479–487

    Article  Google Scholar 

  4. Cockerham G (1955) Strains of potato virus X. In: Proceeding of 2nd conference on potato virus diseases, Lisse-Wageningen, 1954. pp 89–92

  5. Moriera A, Jones RAC, Fribourg CE (1980) Properties of a resistance-breaking strain of potato virus X. Ann Appl Biol 95:93–103

    Article  Google Scholar 

  6. Goulden MG, Köhm BA, Santa Cruz S, Kavanagh TA, Baulcombe DC (1993) A feature of the coat protein of potato virus X affects both induced virus resistance in potato and viral fitness. Virology 197:293–302

    Article  CAS  PubMed  Google Scholar 

  7. Kavanagh T, Goulden M, Santa Cruz S, Chapman S, Barker I, Baulcombe D (1992) Molecular analysis of a resistance-breaking strain of potato virus X. Virol 189:609–617

    Article  CAS  Google Scholar 

  8. Malcuit I, Marano MR, Kavanagh TA, De Jong W, Forsyth A et al (1999) The 25 kDa movement protein of PVX elicits Nb-mediated hypersensitive cell death in potato. Mol Plant Microbe Interact 12:536–543

    Article  CAS  Google Scholar 

  9. Santa Cruz S, Baulcombe DC (1995) Analysis of potato virus X coat protein genes in relation to resistance conferred by the genes Nx, Nb and Rx1 of potato. J Gen Virol 76:2057–2061

    Article  CAS  PubMed  Google Scholar 

  10. Malcuit I, de Jong W, Baulcombe DC, Shields DC, Kavanagh TA (2000) Acquisition of multiple virulence/avirulence determinants by potato virus X (PVX) has occurred through convergent evolution rather than through recombination. Virus Genes 20:165–172

    Article  CAS  PubMed  Google Scholar 

  11. Choi SH, Ryu KH (2008) Determination of complete genome sequence of Korean isolate of Potato virus X. Plant Pathol J 24:361–364

    CAS  Google Scholar 

  12. Esfandiari N, Kohi-Habibi M, Hohn T, Pooggin MM (2009) Complete genome sequence of an Iranian isolate of Potato virus X from the legume plant Pisum sativum. Virus Genes 39:141–145

    Article  CAS  PubMed  Google Scholar 

  13. Komatsu K, Kagiwada S, Takahashi S, Mori T, Yamaji Y et al (2005) Phylogenetic characteristics, genomic heterogeneity and symptomatic variation of five closely related Japanese strains of Potato virus X. Virus Genes 31:99–105

    Article  CAS  PubMed  Google Scholar 

  14. Ravin NV, Mardanova ES, Kotlyarov RYu, Novikov VK, Atabekov JG et al (2008) Complete sequencing of Potato virus X new strain genome and construction of viral vector for production of target proteins in plants. Biochem (Moscow) 73:44–49

    Article  CAS  Google Scholar 

  15. Yu X-Q, Wang H-Y, Lan Y-F, Zhu X-P, Li X-D et al (2008) Complete genome sequence of a Chinese isolate of Potato virus X and analysis of genetic diversity. J Phytopathol 156:346–351

    Article  CAS  Google Scholar 

  16. Jones RAC (1985) Further studies on resistance breaking strains of potato virus X. Plant Pathol 34:182–189

    Article  Google Scholar 

  17. Bald JG, Pugsley AT (1941) The main virus diseases of the potato in Victoria. Counc Sci Ind Res (Aust) Pamphlet 110:1–40

    Google Scholar 

  18. Santa Cruz S, Baulcombe DC (1993) Molecular analysis of potato virus X isolates in relation to the potato hypersensitivity gene Nx. Mol Plant Microbe Interact 6:707–714

    Google Scholar 

  19. Jones RAC (1982) Breakdown of potato virus X resistance gene Nx: selection of a group four strain from strain group three. Plant Pathol 31:325–331

    Article  Google Scholar 

  20. Tozzini AC, Ceriani MF, Cramer P, Palva ET, Hopp HE (1994) PVX MS, a new strain of potato virus that overcomes the extreme resistance gene Rx. J Phytopathol 141:241–248

    Article  Google Scholar 

  21. Boonham N, Walsh K, Smith P, Madagan K, Graham I et al (2003) Detection of potato viruses using microarray technology: towards a generic method for plant viral disease diagnosis. J Virol Methods 108:181–187

    Article  CAS  PubMed  Google Scholar 

  22. Huisman MJ, Linthorst HJM, Bol JF, Cornelissen BJC (1988) The complete nucleotide sequence of potato virus X and its homologies at the amino acid level with various plus-stranded RNA viruses. J Gen Virol 69:1789–1798

    Article  CAS  PubMed  Google Scholar 

  23. Truve E, Järvekülg L, Bouscaren M-L, Aaspöllu A, Priimagi A et al (1995) Different propagation levels of potato virus X (PVX) isolates in PVX coat protein expressing tobacco plants and protoplasts do not correlate with coat protein sequence similarities. Arch. Phytopathol. Plant Prot. 30:15–30

    Article  CAS  Google Scholar 

  24. Skryabin KG, Kraev AS, SYu Morozov, Rozanov MN, Chernov BK et al (1988) The nucleotide sequence of potato virus X RNA. Nucleic Acids Res 16:10929–10930

    Article  CAS  PubMed  Google Scholar 

  25. Kraev AS, Morozov SY, Lukasheva LI, Rozanov MN, Chernov BK et al (1988) Primary structure and organization of the genome of potato X virus. Dokl Akad Nauk SSSR 300:711–716

    CAS  PubMed  Google Scholar 

  26. Kagiwada S, Yamaji Y, Nakabayashi H, Ugaki M, Namba S (2002) The complete nucleotide sequence of Potato virus X strain OS: the first complete sequence of a Japanese isolate. J Gen Plant Pathol 68:94–98

    Article  CAS  Google Scholar 

  27. Jung HW, Yun WS, Seo HW, Hahm YI (2000) Characterization and partial nucleotide sequence of potato virus X isolated from potato in Korea. Plant Pathol J 16:110–117

    Google Scholar 

  28. Wang C-x, Gao Q, Pan N-s, Chen Z-l (1991) The cDNA cloning and nucleotide sequence of potato virus X coat protein gene. Acta Bot Sin 33:363–369

    CAS  Google Scholar 

  29. Du Z-Y, Chen J-S (2003) Identification and grouping study of Potato virus X Hunan isolate. Vir Sin 18:119–123

    CAS  Google Scholar 

  30. Li XS, Cao YY, Zhou T, Cheng YQ, Li HF et al (2008) Orychophragmus violaceus, a new host of Potato virus X, reported from China. Plant Pathol 57:395

    Article  Google Scholar 

  31. Soliman AM, Barsoum BN, Mohamed GG, El-Attar AK, Mazyad HM (2006) Expression of the coat protein gene of the Egyptian isolate of Potato virus X in Escherichia coli and production of polyclonal antibodies against it. Arab J Biotech 9:115–128

    Google Scholar 

  32. Morozov SYu, Zakhar’ev VM, Chernov BK, Prasolov VS, Kozlov YV et al (1983) Analysis of primary structure and localization of the coat protein gene in genomic RNA of potato X virus. Dokl Akad Nauk SSSR 271:211–215

    CAS  Google Scholar 

  33. Mandel AM, Orman BE, Celnik R, Torres HN, Mentaberry AN (1989) Nucleotide cDNA sequence coding for the PVXc coat protein. Nucleic Acids Res 17:1265

    Article  CAS  PubMed  Google Scholar 

  34. Orman BE, Celnik RM, Mandel AM, Torres HN, Mentaberry AN (1990) Complete cDNA sequence of a South American isolate of potato virus X. Virus Res 16:293–305

    Article  CAS  PubMed  Google Scholar 

  35. Querci M, van der Vlugt R, Goldbach R, Salazar LF (1993) RNA sequence of potato virus X strain HB. J Gen Virol 74:2251–2255

    Article  CAS  PubMed  Google Scholar 

  36. Feigelstock DA, Tozzini AC, Hopp HE (1995) Coat protein sequence of a resistance-breaking strain of potato virus X isolated in Argentina. Virus Genes 10:289–292

    Article  CAS  PubMed  Google Scholar 

  37. Torrance L, Larkins AP, Butcher GW (1986) Characterisation of monoclonal antibodies against potato virus X and comparison of serotypes within resistance groups. J Gen Virol 67:67

    Google Scholar 

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Acknowledgments

In addition to those mentioned in the text, we thank Mark Holland, Geoffrey Dwyer and Michael Jones for help in obtaining research funding, and Stuart Vincent for technical support. This research was funded by Australian Research Council Linkage Grant LP0668429 between Murdoch University, the Department of Agriculture and Food Western Australia, Saturn biotech Ltd., and the Western Australian Potato Producers Committee of the Australian Agricultural Produce Commission.

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Authors and Affiliations

  1. Western Australian State Agricultural Biotechnology Centre, Murdoch University, Perth, WA, 6150, Australia

    Belinda A. Cox & Roger A. C. Jones

  2. Department of Agriculture and Food, Bentley Delivery Centre, Locked Bag No. 4, Perth, WA, 6983, Australia

    Roger A. C. Jones

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  1. Belinda A. Cox
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  2. Roger A. C. Jones
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Correspondence to Roger A. C. Jones.

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Cox, B.A., Jones, R.A.C. Genetic variability in the coat protein gene of Potato virus X and the current relationship between phylogenetic placement and resistance groupings. Arch Virol 155, 1349–1356 (2010). https://doi.org/10.1007/s00705-010-0711-3

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  • DOI: https://doi.org/10.1007/s00705-010-0711-3

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