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Genetic and biological characterization of avian influenza H5N1 viruses isolated from wild birds and poultry in Western Siberia

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Abstract

Three viruses included in the study were isolated from dead birds (A/duck/Omsk/1822/2006, A/chicken/Reshoty/02/2006, and A/duck/Tuva/01/2006), whereas the virus A/common gull/Chany/P/2006 was isolated from an apparently healthy gull during outbreaks of highly pathogenic avian influenza in Russia in 2006. The intravenous pathogenicity index (IVPI) of viruses A/duck/Omsk/1822/2006, A/chicken/Reshoty/02/2006, and A/duck/Tuva/01/2006 ranged from 2.7 to 3.0, while the virus A/common gull/Chany/P/2006 had a markedly lower IVPI of 1.7. The virus A/common gull/Chany/P/2006 had a unique pattern of six amino acid substitutions in the regions of viral proteins crucial for virulence of H5N1 viruses. We hypothesize that these substitutions may affect the pathogenicity of A/common gull/Chany/P/2006.

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Acknowledgments

The authors would like to thank Dr. Gavin J. Smith, Dr. Yi Guan, Dr. Malik J.S. Peiris, State Key Laboratory of Emerging Infectious Diseases & Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China, and Dr. Erica Spackman, Dr. David E. Swayne, Southeast Poultry Research Laboratory US Department of Agriculture, Athens, Georgia, for valuable support. This study was supported by a grant BII ISTC 3436 and by Russian Government.

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  1. FSRI SRC VB “Vector”, Novosibirsk, Russia

    Kirill Sharshov, Alesia Romanovskaya, Roman Uzhachenko, Alexander Durymanov, Anna Zaykovskaya, Olga Kurskaya, Philipp Ilinykh, Nikita Silko, Mikhail Kulak, Alexander Alekseev, Sergei Zolotykh, Alexander Shestopalov & Iliya Drozdov

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Sharshov, K., Romanovskaya, A., Uzhachenko, R. et al. Genetic and biological characterization of avian influenza H5N1 viruses isolated from wild birds and poultry in Western Siberia. Arch Virol 155, 1145–1150 (2010). https://doi.org/10.1007/s00705-010-0676-2

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  • DOI: https://doi.org/10.1007/s00705-010-0676-2

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