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Sequence analysis of RNA 2 and RNA 3 of lilac leaf chlorosis virus: a putative new member of the genus Ilarvirus

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Abstract

RNA 2 and RNA 3 of lilac leaf chlorosis virus (LLCV) were sequenced and shown to be 2,762 nucleotides (nt) and 2,117 nts in length, respectively. RNA 2 encodes a putative 807-amino-acid (aa) RNA-dependent RNA polymerase associated protein with an estimated M r of 92.75 kDa. RNA 3 is bicistronic, with ORF1 encoding a putative movement protein (277 aa, M r 31.45 kDa) and ORF2 encoding the putative coat protein (221 aa, M r 24.37 kDa). The genome organization is similar to that typical for members of the genus Ilarvirus. Phylogenetic analyses indicate a close evolutionary relationship between LLCV, ApMV, and PNRSV.

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Authors and Affiliations

  1. Sidney Laboratory, Centre for Plant Health, Canadian Food Inspection Agency, 8801 East Saanich Road, Sidney, BC, V8L 1H3, Canada

    D. James, A. Varga, L. Leippi, S. Godkin & C. Masters

Authors
  1. D. James
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  2. A. Varga
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  3. L. Leippi
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  4. S. Godkin
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  5. C. Masters
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Corresponding author

Correspondence to D. James.

Additional information

The nucleotide sequences have been deposited in EMBL Nucleotide Sequence Database under accession numbers FN669168 (RNA 2) and FN669169 (RNA 3).

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James, D., Varga, A., Leippi, L. et al. Sequence analysis of RNA 2 and RNA 3 of lilac leaf chlorosis virus: a putative new member of the genus Ilarvirus . Arch Virol 155, 993–998 (2010). https://doi.org/10.1007/s00705-010-0673-5

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  • DOI: https://doi.org/10.1007/s00705-010-0673-5

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