Skip to main content
SpringerLink
Log in

Isolation and sequence analysis of Culex flavivirus from Culex interrogator and Culex quinquefasciatus in the Yucatan Peninsula of Mexico

  • Brief Report
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Previously, we reported a high prevalence of Culex flavivirus (CxFV) in Culex quinquefasciatus (Say) in the Yucatan Peninsula of Mexico. To determine whether other Culex spp. mosquitoes in this region are susceptible to natural CxFV infection, Cx. bahamensis (Dyar and Knab), Cx. coronator (Dyar and Knab), Cx. interrogator (Dyar and Knab), Cx. nigripalpus (Theobald) and Cx. opisthopus (Komp) in the Yucatan Peninsula of Mexico were tested for CxFV. Two pools of Cx. interrogator were positive. The envelope protein genes of these isolates and 16 isolates from Cx. quinquefasciatus were sequenced and shown to have ≥99.2% nucleotide identity. These data suggest that there is limited genetic diversity among CxFV isolates in the Yucatan Peninsula of Mexico.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

References

  1. Biggerstaff B (2006) Software for mosquito surveillance (cited February 15, 2010). Centers for Disease Control and Prevention, Fort Collins, Colorado. Available from http://www.cdc.gov/ncidod/dvbid/westnile/software.htm

  2. Blitvich BJ, Lin M, Dorman KS, Soto V, Hovav E, Tucker BJ, Staley M, Platt KB, Bartholomay LC (2009) Genomic sequence and phylogenetic analysis of Culex flavivirus, an insect-specific flavivirus, isolated from Culex pipiens (Diptera: Culicidae) in Iowa. J Med Entomol 46:934–941

    Article  CAS  PubMed  Google Scholar 

  3. Cook S, Bennett SN, Holmes EC, De Chesse R, Moureau G, de Lamballerie X (2006) Isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from Puerto Rico. J Gen Virol 87:735–748

    Article  CAS  PubMed  Google Scholar 

  4. Cook S, Moureau G, Harbach R, Mukwaya L, Goodger K, Ssenfuka F, Gould E, Holmes EC, de Lamballerie X (2009) Isolation of a new species of flavivirus and a novel strain of Culex flavivirus (Flaviviridae), from a natural mosquito population in Uganda. J Gen Virol 90:2669–2678

    Article  CAS  PubMed  Google Scholar 

  5. Crabtree MB, Nga PT, Miller BR (2009) Isolation and characterization of a new mosquito flavivirus, Quang Binh virus, from Vietnam. Arch Virol 154:857–860

    Article  CAS  PubMed  Google Scholar 

  6. Crabtree MB, Sang RC, Stollar V, Dunster LM, Miller BR (2003) Genetic and phenotypic characterization of the newly described insect flavivirus, Kamiti River virus. Arch Virol 148:1095–1118

    Article  CAS  PubMed  Google Scholar 

  7. Crochu S, Cook S, Attoui H, Charrel RN, De Chesse R, Belhouchet M, Lemasson JJ, de Micco P, de Lamballerie X (2004) Sequences of flavivirus-related RNA viruses persist in DNA form integrated in the genome of Aedes spp. mosquitoes. J Gen Virol 85:1971–1980

    Article  CAS  PubMed  Google Scholar 

  8. Darsie RF Jr (1996) A survey and bibliography of the mosquito fauna of Mexico (Diptera: Culicidae). J Am Mosq Control Assoc 12:298–306

    PubMed  Google Scholar 

  9. Farfan-Ale JA, Lorono-Pino MA, Garcia-Rejon JE, Soto V, Lin M, Staley M, Dorman KS, Bartholomay LC, Hovav E, Blitvich BJ (2010) Detection of flaviviruses and orthobunyaviruses in mosquitoes in the Yucatan Peninsula of Mexico in 2008. Vector Borne Zoonotic Dis, (in press)

  10. Farfan-Ale JA, Lorono-Pino MA, Garcia-Rejon JE, Hovav E, Powers AM, Lin M, Dorman KS, Platt KB, Bartholomay LC, Soto V, Beaty BJ, Lanciotti RS, Blitvich BJ (2009) Detection of RNA from a Novel West Nile-like virus and high prevalence of an insect-specific flavivirus in mosquitoes in the Yucatan Peninsula of Mexico. Am J Trop Med Hyg 80:85–95

    CAS  PubMed  Google Scholar 

  11. Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244

    Article  CAS  PubMed  Google Scholar 

  12. Hoshino K, Isawa H, Tsuda Y, Sawabe K, Kobayashi M (2009) Isolation and characterization of a new insect flavivirus from Aedes albopictus and Aedes flavopictus mosquitoes in Japan. Virology 391:119–129

    Article  CAS  PubMed  Google Scholar 

  13. Hoshino K, Isawa H, Tsuda Y, Yano K, Sasaki T, Yuda M, Takasaki T, Kobayashi M, Sawabe K (2007) Genetic characterization of a new insect flavivirus isolated from Culex pipiens mosquito in Japan. Virology 359:405–414

    Article  CAS  PubMed  Google Scholar 

  14. Huhtamo E, Putkuri N, Kurkela S, Manni T, Vaheri A, Vapalahti O, Uzcategui NY (2009) Characterization of a novel flavivirus from mosquitoes in northern europe that is related to mosquito-borne flaviviruses of the tropics. J Virol 83:9532–9540

    Article  CAS  PubMed  Google Scholar 

  15. Junglen S, Kopp A, Kurth A, Pauli G, Ellerbrok H, Leendertz FH (2009) A new flavivirus and a new vector: characterization of a novel flavivirus isolated from uranotaenia mosquitoes from a tropical rain forest. J Virol 83:4462–4468

    Article  CAS  PubMed  Google Scholar 

  16. Kihara Y, Satho T, Eshita Y, Sakai K, Kotaki A, Takasaki T, Rongsriyam Y, Komalamisra N, Srisawat R, Lapcharoen P, Sumroiphon S, Iwanaga S, Ushijima H, Endoh D, Miyata T, Sakata A, Kashige N, Miake F, Fukushi S, Saijo M, Kurane I, Morikawa S, Mizutani T (2007) Rapid determination of viral RNA sequences in mosquitoes collected in the field. J Virol Methods 146:372–374

    Article  CAS  PubMed  Google Scholar 

  17. Kim DY, Guzman H, Bueno R Jr, Dennett JA, Auguste AJ, Carrington CV, Popov VL, Weaver SC, Beasley DW, Tesh RB (2009) Characterization of Culex Flavivirus (Flaviviridae) strains isolated from mosquitoes in the United States and Trinidad. Virology 386:154–159

    Article  CAS  PubMed  Google Scholar 

  18. Kuno G, Chang GJ, Tsuchiya KR, Karabatsos N, Cropp CB (1998) Phylogeny of the genus Flavivirus. J Virol 72:73–83

    CAS  PubMed  Google Scholar 

  19. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23:2947–2948

    Article  CAS  PubMed  Google Scholar 

  20. Morales-Betoulle ME, Monzon Pineda ML, Sosa SM, Panella N, Lopez MR, Cordon-Rosales C, Komar N, Powers A, Johnson BW (2008) Culex flavivirus isolates from mosquitoes in Guatemala. J Med Entomol 45:1187–1190

    Article  CAS  PubMed  Google Scholar 

  21. Moureau GL, Ninove A, Izri S, Cook X, De Lamballerie, Charrel RN (2010) Flavivirus RNA in Phlebotomine Sandflies. Vector Borne Zoonotic Dis 10:195–197

  22. Roiz D, Vazquez A, Seco MP, Tenorio A, Rizzoli A (2009) Detection of novel insect flavivirus sequences integrated in Aedes albopictus (Diptera: Culicidae) in Northern Italy. Virol J 6:93

    Article  PubMed  Google Scholar 

  23. Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  CAS  PubMed  Google Scholar 

  24. Sanchez-Seco MP, Vazquez A, Collao X, Hernandez L, Aranda C, Ruiz S, Escosa R, Marques E, Bustillo MA, Molero F, Tenorio A (2009) Surveillance of Arboviruses in Spanish Wetlands: detection of new Flavi- and Phleboviruses. Vector Borne Zoonotic Dis

  25. Sang RC, Gichogo A, Gachoya J, Dunster MD, Ofula V, Hunt AR, Crabtree MB, Miller BR, Dunster LM (2003) Isolation of a new flavivirus related to cell fusing agent virus (CFAV) from field-collected flood-water Aedes mosquitoes sampled from a dambo in central Kenya. Arch Virol 148:1085–1093

    Article  CAS  PubMed  Google Scholar 

  26. Stollar V, Thomas VL (1975) An agent in the Aedes aegypti cell line (Peleg) which causes fusion of Aedes albopictus cells. Virology 64:367–377

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This study was supported by grant 5R21AI067281-02 from the National Institutes of Health.

Author information

Authors and Affiliations

  1. Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, 2116 Veterinary Medicine, Ames, IA, 50011, USA

    Rungrat Saiyasombat & Bradley J. Blitvich

  2. Department of Statistics and Genetics, Development and Cell Biology, College of Agriculture and Life Sciences, Iowa State University, Ames, IA, USA

    Karin S. Dorman

  3. Laboratorio de Arbovirologia, The Universidad Autonoma de Yucatan, Merida, Yucatan, Mexico

    Julian E. Garcia-Rejon, Maria A. Loroño-Pino & Jose A. Farfan-Ale

Authors
  1. Rungrat Saiyasombat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karin S. Dorman
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Julian E. Garcia-Rejon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Maria A. Loroño-Pino
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jose A. Farfan-Ale
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Bradley J. Blitvich
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Bradley J. Blitvich.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Saiyasombat, R., Dorman, K.S., Garcia-Rejon, J.E. et al. Isolation and sequence analysis of Culex flavivirus from Culex interrogator and Culex quinquefasciatus in the Yucatan Peninsula of Mexico. Arch Virol 155, 983–986 (2010). https://doi.org/10.1007/s00705-010-0665-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-010-0665-5

Keywords

Search

Navigation