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Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface

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Abstract

Both fibronectin and the asialoglycoprotein receptor (ASGPR) have been identified by some investigators as partners for hepatitis B virus (HBV) envelope proteins. Because fibronectin is a natural ligand for ASGPR, we speculated that HBV might attach to ASGPR expressed on the hepatocyte surface via fibronectin. To test this hypothesis, we first confirmed by co-immunoprecipitation that ASGPR, fibronectin and HBsAg bind to each other in HepG2.2.15 cells, and possible binding domains were identified by GST pull-down. In addition, by measuring binding of HBsAg to cells, we found that ASGPR and fibronectin enhanced the binding capability of HBsAg to HepG2 cells, and even to 293T and CHO cells, which normally do not bind HBV. In conclusion, our findings suggest that both fibronectin and ASGPR mediate HBsAg binding to the cell surface, which provides further evidence for the potential roles of these two proteins in mediating HBV binding to liver cells.

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Abbreviations

ASGPR:

Asialoglycoprotein receptor

HBsAg:

HBV surface antigen

HBV:

Hepatitis B virus

MAb:

Monoclonal antibody

DMEM:

Dulbecco’s modified Eagle’s medium

CRD:

Carbohydrate recognition domain

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Acknowledgments

This work was supported by three grants from the National Natural Science Foundation of China (nos. 30530650, 30600531, and 30625041) and special funding from the Major State Basic Research Program of China (973 program) (no. 2005CB522902). We are grateful to Caibing Cui for providing plasmids expressing FN fragments and to many colleagues for helpful discussion during the course of this work.

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Authors and Affiliations

  1. Beijing Institute of Radiation Medicine, 27 Taiping Road, Haidian District, Beijing, 100850, People’s Republic of China

    Jing Yang, Feng Wang, Linlin Tian, Jing Su, Xiangqian Zhu, Li Lin, Xiaoran Ding, Xuejun Wang & Shengqi Wang

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  1. Jing Yang
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  2. Feng Wang
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  3. Linlin Tian
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  4. Jing Su
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  6. Li Lin
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  7. Xiaoran Ding
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  8. Xuejun Wang
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  9. Shengqi Wang
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Correspondence to Shengqi Wang.

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J. Yang and F. Wang contributed equally to this work.

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Yang, J., Wang, F., Tian, L. et al. Fibronectin and asialoglyprotein receptor mediate hepatitis B surface antigen binding to the cell surface. Arch Virol 155, 881–888 (2010). https://doi.org/10.1007/s00705-010-0657-5

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  • DOI: https://doi.org/10.1007/s00705-010-0657-5

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