Abstract
We have recently shown that porcine reproductive and respiratory syndrome virus (PRRSV) can undergo a productive replication in porcine monocyte-derived dendritic cells (Mo-DCs). Here, we further demonstrate that PRRSV activates the host’s phosphatidylinositol-3-kinase (PI3K)-dependent Akt pathway (PI3K/Akt) to facilitate its replication in Mo-DCs at 90 min and 4 h after infection. Inhibition of PI3K/Akt by treatment with a PI3K-specific inhibitor (LY294002) prior to PRRSV infection reduced virus replication. Furthermore, inhibition of PI3K/Akt by LY294002 at 90 min and 8 h after virus infection still significantly reduced virus production, suggesting that virus replication may be dependent on the activation of PI3K/Akt. Interestingly, PRRSV inhibited PI3K/Akt at 12 h after infection. Heat-inactivated virus failed to inhibit PI3K/Akt, indicating that virus replication is essential for this inhibition. Overall, PRRSV replication exhibits a dual effect on the PI3K/Akt pathway in which both time-dependent activation and inhibition of PI3K/Akt are observed.
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Acknowledgments
We thank Dr. Eric Nelson for providing FITC-SDOW17 antibody. This study was supported by grants from USDA NRI (2009-35204-05079) and by the South Dakota Agricultural Experiment Station.
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Zhang, H., Wang, X. A dual effect of porcine reproductive and respiratory syndrome virus replication on the phosphatidylinositol-3-kinase-dependent Akt pathway. Arch Virol 155, 571–575 (2010). https://doi.org/10.1007/s00705-010-0611-6
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DOI: https://doi.org/10.1007/s00705-010-0611-6