Abstract
Eleven pospiviroid isolates from ornamental plants and one from pepper were successfully transmitted to potato and tomato by mechanical inoculation. All isolates with characteristic predominant genotypes were inoculated to a series of potato and tomato plants and propagated for up to four passages. In total, 385 nucleotide sequences were determined, in which 17 new predominant genotypes were identified with minimal differences to the original predominant genotype. In addition, in the original ornamental hosts, only one of eleven predominant pospiviroid genotypes had changed during the experiments over a period of 2 years. These results confirm the high stability of predominant pospiviroid genotypes.
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Acknowledgments
We thank our colleagues of the department National Reference Laboratory of the Netherlands Plant Protection Service for performing the tests and Ricardo Flores for critically reading of the manuscript.
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Verhoeven, J.T.J., Roenhorst, J.W. High stability of original predominant pospiviroid genotypes upon mechanical inoculation from ornamentals to potato and tomato. Arch Virol 155, 269–274 (2010). https://doi.org/10.1007/s00705-009-0572-9
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DOI: https://doi.org/10.1007/s00705-009-0572-9