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The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection

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Abstract

The porcine reproductive and respiratory syndrome virus (PRRSV) enters its target cell via clathrin-mediated endocytosis. Using dominant-negative Rab5 and Rab7 mutants, we show that upon internalization, PRRSV enters early endosomes but does not continue through the endocytic pathway to late endosomes. This was confirmed via colocalization experiments visualizing PRRSV and markers for different compartments of the endocytic pathway. Furthermore, it was shown that PRRSV colocalizes with its internalization receptor, sialoadhesin, on the cell surface and beneath the plasma membrane, while CD163 and PRRSV only meet in early endosomes.

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Acknowledgments

We gratefully thank G.R. Whittaker (Cornell University, USA) for providing the Rab constructs, and C. Vanmaercke and C. Boone for excellent technical assistance. This work was supported by the Ghent University Industrial Research Fund (IOF).

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820, Merelbeke, Belgium

    Hanne Van Gorp, Wander Van Breedam, Peter L. Delputte & Hans J. Nauwynck

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  1. Hanne Van Gorp
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  2. Wander Van Breedam
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  3. Peter L. Delputte
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  4. Hans J. Nauwynck
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Corresponding author

Correspondence to Peter L. Delputte.

Additional information

P.L. Delputte and H.J. Nauwynck share the senior authorship.

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Van Gorp, H., Van Breedam, W., Delputte, P.L. et al. The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection. Arch Virol 154, 1939–1943 (2009). https://doi.org/10.1007/s00705-009-0527-1

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  • DOI: https://doi.org/10.1007/s00705-009-0527-1

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