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Genetic diversity and classification of the outer capsid glycoprotein VP7 of porcine group B rotaviruses

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Abstract

We determined the nucleotide sequences of the outer capsid glycoprotein (VP7) genes of 38 porcine group B rotaviruses (GBRs) from feces of pigs at 27 farms in Japan between 2000 and 2007. Substantial diversity among porcine GBR VP7 genes was observed, with up to 42.4% difference in nucleotides and 49.8% in amino acids. On comparison of VP7 genes, porcine GBRs were clearly distinct from the published corresponding genes from human, bovine and murine GBRs (53.7–70.8% identity in nucleotides and 45.8–73.4% identity in amino acids). Phylogenetic analysis showed that the VP7s of GBRs could be divided into five genotypes: the murine strain was genotype 1, human strains were genotype 2, bovine and some porcine strains were genotype 3, and other porcine strains belonged to genotype 4 or 5. In addition, GBR VP7s in genotypes 3 and 5 were further divided into four and five clusters, respectively. No relationship between VP7 genotype and double-stranded RNA migration patterns of porcine GBRs in polyacrylamide gel electrophoresis were observed. However, an antigen enzyme-linked immunosorbent assay using antiserum to recombinant bovine GBR VP6 did not react with fecal samples containing one cluster of genotype 5 of porcine GBRs. The abundant divergence of porcine GBR VP7 genes suggests that porcine species might be an original natural host of GBR infection and that different serotypes might exist among porcine GBRs. To our knowledge, this is the first report to describe the gene sequences and typing of porcine GBR VP7s.

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Acknowledgments

We thank M. Kamiyama, M. Kohmoto, K. Kawashima, S. Nishi, T. Aita, T. Obara, Y. Sakai, T. Miyamoto, and N. Sawada for their technical assistance. This research was supported in part by a grant from the National Institute of Animal Health, Japan.

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Authors and Affiliations

  1. Research Team for Viral Diseases, National Institute of Animal Health, Kannondai 3-1-5, Tsukuba, Ibaraki, 3050856, Japan

    Kazufumi Kuga, Ayako Miyazaki, Takako Suzuki, Michihiro Takagi, Nachiko Hattori, Masaji Mase & Hiroshi Tsunemitsu

  2. Research Team for Environmental/Enzootic Diseases, National Institute of Animal Health, Sichinohe, Aomori, 0392586, Japan

    Ken Katsuda

  3. The United Graduate School of Veterinary Sciences, Gifu University, Gifu, 5011193, Japan

    Kazufumi Kuga, Masaji Mase, Makoto Sugiyama & Hiroshi Tsunemitsu

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  1. Kazufumi Kuga
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Correspondence to Hiroshi Tsunemitsu.

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Kuga, K., Miyazaki, A., Suzuki, T. et al. Genetic diversity and classification of the outer capsid glycoprotein VP7 of porcine group B rotaviruses. Arch Virol 154, 1785–1795 (2009). https://doi.org/10.1007/s00705-009-0517-3

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  • DOI: https://doi.org/10.1007/s00705-009-0517-3

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