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New isolates of carnation Italian ringspot virus differ from the original one by having replication-associated proteins with a typical tombusvirus-like N-terminus and by inducing peroxisome- rather than mitochondrion-derived multivesicular bodies

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Abstract

Five new isolates of carnation Italian ringspot virus (CIRV) from cherry trees, Gypsophila and surface water differ from the original carnation isolate (CIRV-car) and also from Pelargonium necrotic spot virus (PelNSV) by having an ORF 1/ORF1-RT with a typical tombusvirus-like 5′end and by inducing the formation of peroxisome- rather than mitochondrion-derived multivesicular bodies (MVBs). This supports with natural isolates earlier conclusions reached by others with artificially produced hybrid viruses that the 5′end of ORF 1 determines from which organelle the MBVs will be derived. CIRV-car might have resulted from a natural recombination event with genome elements of a PelNSV-like virus.

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Acknowledgments

We are greatly indebted to Petra Lüddecke, Christina Maß and Sabine Schuhmann for expert technical assistance and to Dr. G.P Martelli for the original CIRV isolate from Dr. M. Hollings.

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Authors and Affiliations

  1. c/o Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Messeweg 11, 38104, Braunschweig, Germany

    Renate Koenig, Renate Koenig & Dietrich-Eckhardt Lesemann

  2. Julius Kühn Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Nationale und Internationale Angelegenheiten der Pflanzengesundheit, Messeweg 11, 38104, Braunschweig, Germany

    Ernst Pfeilstetter

Authors
  1. Renate Koenig
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  2. Dietrich-Eckhardt Lesemann
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  3. Ernst Pfeilstetter
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Corresponding author

Correspondence to Renate Koenig.

Additional information

The GenBank accession numbers for the Gyp1, K483, OE11, OE14 and RSG isolates of CIRV are GQ259476, GQ259477, GQ259478, GQ259479 and GQ259480, respectively.

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Koenig, R., Lesemann, DE. & Pfeilstetter, E. New isolates of carnation Italian ringspot virus differ from the original one by having replication-associated proteins with a typical tombusvirus-like N-terminus and by inducing peroxisome- rather than mitochondrion-derived multivesicular bodies. Arch Virol 154, 1695–1698 (2009). https://doi.org/10.1007/s00705-009-0491-9

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  • DOI: https://doi.org/10.1007/s00705-009-0491-9

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