Skip to main content

Advertisement

SpringerLink
Log in

Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors

  • Original Article
  • Published:
Archives of Virology Aims and scope Submit manuscript

Abstract

Recombinant baculoviruses (recBV) were constructed with dual cassettes for constitutive expression of human IgG Fc following infection of insect cells and the structural proteins of hepatitis C virus (core, E1 and E2) following transduction of mammalian cells. The IgG Fc was expressed in insect cells as a fusion protein with the signal sequence and transmembrane region of either the native baculovirus envelope protein gp64 or the human transferrin receptor as a type I or type II integral membrane protein, respectively. The IgG Fc fusion proteins formed functional homodimers on the surface of recBV-infected insect cells and were incorporated into the envelope of recBV particles during egress from the infected cell. Both pseudotyped recBV bound specifically to recombinant soluble FcγRIIα receptor and to cell lines and antigen-presenting cells expressing Fc receptors (FcRs). These novel baculoviral vectors, which target cells of the immune system that express FcRs, have potential applications for vaccination or gene therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Abe T, Hemmi H, Miyamoto H, Moriishi K, Tamura S, Takaku H, Akira S, Matsuura Y (2005) Involvement of the toll-like receptor 9 signaling pathway in the induction of innate immunity by baculovirus. J Virol 79:2847–2858

    Article  PubMed  CAS  Google Scholar 

  2. Beard MR, Abell G, Honda M, Carroll A, Gartland M, Clarke B, Suzuki K, Lanford R, Sangar DV, Lemon SM (1999) An infectious molecular clone of a Japanese genotype 1b hepatitis C virus. Hepatology 30:316–324

    Article  PubMed  CAS  Google Scholar 

  3. Belousova N, Korokhov N, Krendelshchikova V, Simonenko V, Mikheeva G, Triozzi PL, Aldrich WA, Banerjee PT, Gillies SD, Curiel DT, Krasnykh V (2003) Genetically targeted adenovirus vector directed to CD40-expressing cells. J Virol 77:11367–11377

    Article  PubMed  CAS  Google Scholar 

  4. Condreay JP, Witherspoon SM, Clay WC, Kost TA (1999) Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector. Proc Natl Acad Sci USA 96:127–132

    Article  PubMed  CAS  Google Scholar 

  5. Facciabene A, Aurisicchio L, La Monica N (2004) Baculovirus vectors elicit antigen-specific immune responses in mice. J Virol 78:8663–8672

    Article  PubMed  CAS  Google Scholar 

  6. Grabherr R, Ernst W, Doblhoff-Dier O, Sara M, Katinger H (1997) Expression of foreign proteins on the surface of Autographa californica nuclear polyhedrosis virus. BioTechniques 22:730–735

    PubMed  CAS  Google Scholar 

  7. Grabherr R, Ernst W, Oker-Blom C, Jones I (2001) Developments in the use of baculoviruses for the surface display of complex eukaryotic proteins. Trends Biotechnol 19:231–236

    Article  PubMed  CAS  Google Scholar 

  8. Hofman C, Sandig V, Jennings G, Rudolph M, Schlag P, Strauss M (1995) Efficient gene transfer into human hepatocytes by baculovirus vectors. Proc Natl Acad Sci USA 92:10099–10103

    Article  Google Scholar 

  9. Kalergis AM, Ravetch JV (2002) Inducing tumor immunity through the selective engagement of activating Fcγ receptors on dendritic cells. J Exp Med 195:1653–1659

    Article  PubMed  CAS  Google Scholar 

  10. Kitagawa Y, Tani H, Limn CK, Matsunaga TM, Moriishi K, Matsuura Y (2005) Ligand-directed gene targeting to mammalian cells by pseudotype baculoviruses. J Virol 79:3639–3652

    Article  PubMed  CAS  Google Scholar 

  11. Korokhov N, de Gruijl TD, Aldrich WA, Triozzi PL, Banerjee PT, Gillies SD, Curiel TJ, Douglas JT, Scheper JR, Curiel DT (2005) High efficiency transduction of dendritic cells by adenoviral vectors targeted to DC-SIGN. Cancer Biol and Ther 4:289–294

    Article  CAS  Google Scholar 

  12. Leopold PL, Wendland RL, Vincent T, Crystal RG (2006) Neutralized adenovirus-immune complexes can mediate effective gene transfer via an Fc receptor-dependent infection pathway. J Virol 80:10237–10247

    Article  PubMed  CAS  Google Scholar 

  13. Maguire CA, Sapinoro R, Girgis N, Rodriguez-Colon SM, Ramirez SH, Williams J, Dewhurst S (2006) Recombinant adenovirus type 5 vectors that target DC-SIGN, ChemR23 and αvβ3 integrin efficiently transduce human dendritic cells and enhance presentation of vectored antigens. Vaccine 24:671–682

    PubMed  CAS  Google Scholar 

  14. Makela AR, Matilainen H, White DJ, Ruoslahti E, Oker-Blom C (2006) Enhanced baculovirus-mediated transduction of human cancer cells by tumor-homing peptides. J Virol 80:6603–6611

    Article  PubMed  CAS  Google Scholar 

  15. Martyn JC, Dong X, Holmes-Brown S, Pribul P, Li S, Drummer HE, Gowans EJ (2007) Transient and stable expression of the HCV envelope glycoproteins in cell lines and primary hepatocytes transduced with a recombinant baculovirus. Arch Virol 152:329–343

    Article  PubMed  CAS  Google Scholar 

  16. Monsma SA, Blissard GW (1995) Identification of a membrane fusion domain and an oligomerization domain in the baculovirus gp64 envelope fusion protein. J Virol 69:2583–2595

    PubMed  CAS  Google Scholar 

  17. Ojala K, Mottershead SuokkoA, Oker-Blom C (2001) Specific binding of baculoviruses displaying gp64 fusion proteins to mammalian cells. Biochem Biophys Res Commun 284:777–784

    Article  PubMed  CAS  Google Scholar 

  18. Ortiz-Stern A, Rosales C (2003) Cross-talk between Fc receptors and integrins. Immunol Lett 90:137–143

    Article  PubMed  CAS  Google Scholar 

  19. Powell MS, Barnes NC, Bradford TM, Musgrave IF, Wines BD, Cambier JC, Hogarth PM (2006) Alteration of the Fc gamma RIIa dimer interface affects receptor signalling but not ligand binding. J Immunol 176:7489–7494

    PubMed  CAS  Google Scholar 

  20. Schutz A, Scheller N, Breinig T, Meyerhans A (2006) The Autographa californica nuclear polyhedrosis virus AcNPV induces functional maturation of human monocyte-derived dendritic cells. Vaccine 24:7190–7196

    Article  PubMed  CAS  Google Scholar 

  21. Stabila PF, Wong SC, Kaplan FA, Tao W (1998) Cell surface expression of a human IgG Fc chimera activates macrophages through Fc receptors. Nat Biotechnol 16:1357–1360

    Article  PubMed  CAS  Google Scholar 

  22. Strauss R, Huser A, Ni S, Tuve S, Kiviat N, Sow PS, Hofmann C, Lieber A (2007) Baculovirus-based vaccination vectors allow for efficient induction of immune responses against Plasmodium falciparum circumsporozoite protein. Mol Ther 15:193–202

    Article  PubMed  CAS  Google Scholar 

  23. Tacken PJ, Torensma R, Figdor CG (2006) Targeting antigens to dendritic cells in vivo. Immunobiology 211:599–608

    Article  PubMed  CAS  Google Scholar 

  24. Tani H, Limn CK, Yap CC, Onishi M, Nozaki M, Nishimune Y, Okahashi N, Kitagawa Y, Watanabe R, Mochizuki R, Moriishi K, Matsuura Y (2003) In vitro and in vivo gene delivery by recombinant baculoviruses. J Virol 77:9799–9808

    Article  PubMed  CAS  Google Scholar 

  25. Zerial M, Melancon P, Schneider C, Garoff H (1986) The transmembrane segment of the human transferrin receptor functions as a signal peptide. EMBO J 5:1543–1550

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. Patrick Condreay for the kind gift of plasmid pFastBacMam (pFBM) and Professor Stan Lemon for plasmid pHCV-N. This study was supported by a grant from the National Health and Medical Research Council of Australia. The HCV Laboratory at the Burnet Institute is a member of the Australian Centre for Hepatitis Virology and the Australian Centre for HIV and Hepatitis Virology.

Author information

Authors and Affiliations

  1. The Macfarlane Burnet Institute for Medical Research and Public Health, GPO Box 2284, Melbourne, VIC, 3001, Australia

    John C. Martyn, Anthony J. Cardin, Bruce D. Wines, Angela Cendron, Shuo Li, Maree Powell & Eric J. Gowans

  2. School of Molecular and Microbial Sciences, University of Queensland, St. Lucia, Brisbane, QLD, 4072, Australia

    Jason Mackenzie

  3. Department of Microbiology and Immunology, Monash University, Clayton, VIC, 3800, Australia

    Eric J. Gowans

Authors
  1. John C. Martyn
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anthony J. Cardin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Bruce D. Wines
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Angela Cendron
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Shuo Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jason Mackenzie
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Maree Powell
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Eric J. Gowans
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eric J. Gowans.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Martyn, J.C., Cardin, A.J., Wines, B.D. et al. Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors. Arch Virol 154, 1129–1138 (2009). https://doi.org/10.1007/s00705-009-0423-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-009-0423-8

Keywords

Search

Navigation