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Infectious RNA transcripts derived from cloned cDNA of a pepino mosaic virus isolate

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Abstract

For the first time, a full-length cDNA clone of the RNA genome of pepino mosaic virus (PepMV) was constructed. RNA was extracted from purified virions of isolate PepMV-Pa and used for cDNA synthesis. The full-length cDNA was produced as one 6.4-kb fragment representing the entire PepMV genome. This fragment was ligated into the pCR® -XL-TOPO® vector downstream of T7 RNA polymerase promoter, which was included in the 5′ primer sequence used for RT-PCR. The PepMV-Pa RNA transcripts obtained were infectious in different host plants, causing symptoms indistinguishable from those of the wild-type isolate. The presence and authenticity of the progeny virus were verified by ELISA, RT-PCR and nucleotide sequencing.

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References

  1. Aguilar JM, Hernandez-Gallardo MD, Cenis JL, Lacasa A, Aranda MA (2002) Complete sequence of the pepino mosaic virus RNA genome. Arch Virol 147:2009–2015

    Article  PubMed  CAS  Google Scholar 

  2. Cotillon AC, Girard M, Ducouret S (2002) Complete nucleotide sequence of the genomic RNA of a French isolate of (pepino mosaic virus). Arch Virol 147(11):2231–2238

    Article  PubMed  CAS  Google Scholar 

  3. Hanssen IM, Paeleman A, Wittemans L, Goen K, Lievens B, Bragard C, Vanachter ACRC, Thomma BPHJ (2008) Genetic characterization of pepino mosaic virus isolates from Belgian greenhouse tomatoes reveals genetic recombination. Eur J Plant Pathol 121:131–146

    Article  Google Scholar 

  4. Hasiów B, Borodynko N, Pospieszny H (2008) Complete genomic RNA sequence of the Polish pepino mosaic virus isolate belonging to the US2 strain. Virus Genes 36:1–8

    Article  Google Scholar 

  5. Hasiów-Jaroszewska B, Pospieszny H, Borodynko N (2009) New necrotic isolates of pepino mosaic virus representing the Ch2 genotype. J Phytopath (in press), doi:10.1111/j.1439-0434.2008.01496.x

  6. Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brie Bioinform 5:150–163

    Article  CAS  Google Scholar 

  7. Ling KS (2007) Molecular characterization of two pepino mosaic virus variants from imported tomato seed reveals high levels of sequence identity between Chilean and US isolates. Virus Genes 34:1–8

    Article  PubMed  CAS  Google Scholar 

  8. Mumford RA, Metcalfe EJ (2001) The partial sequencing of genomic RNA of a UK isolate of pepino mosaic virus and the comparison of the coat protein sequence with other isolates from Europe and Peru. Arch Virol 146:2455–2460

    Article  PubMed  CAS  Google Scholar 

  9. Maroon-Lango CJ, Guaragna MA, Jordan RL, Hammond J, Bandla M, Marquardt SK (2005) Two unique US isolates of pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (European–like) US isolate. Arch Virol 150:1187–1201

    Article  PubMed  CAS  Google Scholar 

  10. Pagan I, Cordoba-Selles MC, Martinez-Priego L, Fraile A, Malpica JM, Jorda C, Garcia-Arenal F (2006) Genetic structure of the population of pepino mosaic virus infecting tomato crops in Spain. Phytopathology 96:274–279

    Article  PubMed  CAS  Google Scholar 

  11. Pospieszny H, Borodynko N (2006) New Polish isolate of pepino mosaic virus highly distinct from European tomato, Peruvian, and US2 strains. Plant Dis 90:1106

    Article  Google Scholar 

  12. Pospieszny H, Borodynko N, Palczewska M (2003) First record of pepino mosaic virus in Poland. J Plant Dis Protect 100:97

    Google Scholar 

  13. Pospieszny H, Hasiów B, Borodynko N (2008) Characterization of two distinct Polish isolates of pepino mosaic virus. Eur J Plant Pathol 36:443–445

    Article  Google Scholar 

  14. Pospieszny H, Hasiów B, Borodynko N (2008) Variability of the Polish pepino mosaic virus isolates. The 3rd conference of the international working group on legume and vegetable viruses (IWGLVV). Ljubljana, Slovenia. Book of Abstract, p 65

  15. Verhoeven JThJ, van der Vlugt RAA, Roenhorst JW (2003) High similarity between tomato isolates of pepino mosaic virus suggests a common origin. Eur J Plant Pathol 109:419–425

    Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by Polish Ministry of Science within the project N N 302 312 934.

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Authors and Affiliations

  1. Department of Virology and Bacteriology, Institute of Plant Protection, National Research Institute, ul. Węgorka 20, 60-318, Poznan, Poland

    Beata Hasiów-Jaroszewska, Natasza Borodynko & Henryk Pospieszny

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  1. Beata Hasiów-Jaroszewska
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  2. Natasza Borodynko
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  3. Henryk Pospieszny
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Correspondence to Beata Hasiów-Jaroszewska.

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Hasiów-Jaroszewska, B., Borodynko, N. & Pospieszny, H. Infectious RNA transcripts derived from cloned cDNA of a pepino mosaic virus isolate. Arch Virol 154, 853–856 (2009). https://doi.org/10.1007/s00705-009-0368-y

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  • DOI: https://doi.org/10.1007/s00705-009-0368-y

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