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Complete genomic characterization of a European type 1 porcine reproductive and respiratory syndrome virus isolate in Korea

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) isolates belonging to the European genotype 1 have recently emerged in South Korea, suggesting potential problems for disease control. In the present study, we attempted to determine the complete nucleotide sequence of the first Korean type 1 PRRSV isolate, designated KNU-07. The full-length genome of KNU-07 was found to be 15,038 nucleotides in length, which was 60 nucleotides shorter than the type 1 prototype strain Lelystad due to a notable 60-bp deletion within the nonstructural protein 2 (NSP2). The KNU-07 genome was shown to consist of a 221-nucleotide (nt) 5′ untranslated region (UTR), a 14,703-nt protein-coding region, and a 114-nt 3′ UTR, followed by a 42-73-bp poly(A) tail. A nucleotide sequence comparison of the KNU-07 genome with 20 complete PRRSV genomes revealed a 10.5–13.3% and 39.5–40.3% divergence from type 1 and type 2 strains, respectively, at the genome level, indicating a high similarity to the virus strains commonly identified as the European genotype. In order to investigate genetic variation and to understand the molecular evolution of the type 1 isolate in Korea, extensive phylogenetic analyses were performed using the ORF5 and ORF7 nucleotide sequences of published type 1 PRRSV isolates. The data further indicated that the newly emerging type 1 isolate KNU-07 belongs to the recently proposed pan-European subtype 1. Taken together, the results of this study describe the genomic characterization of the type 1 PRRSV isolated in South Korea, suggesting a recent introduction of the virus typical for this genotype that has commonly appeared worldwide.

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References

  1. Albina E (1997) Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview. Vet Microbiol 55:309–316

    Article  PubMed  CAS  Google Scholar 

  2. Bautista EM, Faaberg KS, Mickelson D, McGruder ED (2002) Functional properties of the predicted helicase of porcine reproductive and respiratory syndrome virus. Virology 298:258–270

    Article  PubMed  CAS  Google Scholar 

  3. Benfield DA, Nelson E, Collins JE, Harris L, Goyal SM, Robison D, Christianson WT, Morrison RB, Gorcyca D, Chladek D (1992) Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J Vet Diagn Invest 4:127–133

    PubMed  CAS  Google Scholar 

  4. Cavanagh D (1997) Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch Virol 142:629–633

    PubMed  CAS  Google Scholar 

  5. Dewey C, Charbonneau G, Carman S, Hamel A, Nayar G, Friendship R, Eernisse K, Swenson S (2000) Lelystad-like strain of porcine reproductive and respiratory syndrome virus (PRRSV) identified in Canadian swine. Can Vet J 41:493–494

    PubMed  CAS  Google Scholar 

  6. Fang Y, Rowland RRR, Roof M, Lunney JK, Christopher-Hennings J, Nelson EA (2006) A full-length cDNA infectious clone of North American type 1 porcine reproductive and respiratory syndrome virus: expression of green fluorescent protein in the Nsp2 region. J Virol 80:11447–11455

    Article  PubMed  CAS  Google Scholar 

  7. Keffaber KK (1989) Reproductive failure of unknown etiology. Am Assoc Swine Pract Newslett 1:1–9

    Google Scholar 

  8. Key KF, Haqshenas G, Guenette DK, Swenson SL, Toth TE, Meng XJ (2001) Genetic variation and phylogenetic analyses of the ORF5 gene of acute porcine reproductive and respiratory syndrome virus isolates. Vet Microbiol 83:249–263

    Article  PubMed  CAS  Google Scholar 

  9. Kim JY, Lee SY, Sur JH, Lyoo YS (2006) Serological and genetic characterization of the European strain of the PRRSV isolated in Korea. Korean J Vet Res 46:363–370

    Google Scholar 

  10. Lee C, Calvert JG, Welch SW, Yoo D (2005) A DNA-launched reverse genetics system for porcine reproductive and respiratory syndrome virus reveals that homodimerization of the nucleocapsid protein is essential for virus infectivity. Virology 331:47–62

    Article  PubMed  CAS  Google Scholar 

  11. Madsen KG, Hansen CM, Madsen ES, Strandbygaard B, Botner A, Sorensen KJ (1998) Sequence analysis of porcine reproductive and respiratory syndrome virus of the American type collected from Danish swine herds. Arch Virol 143:1683–1700

    Article  PubMed  CAS  Google Scholar 

  12. Meng XJ, Paul PS, Halbur PG, Lum MA (1995) Phylogenetic analysis of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): implication for the existence of two genotypes of PRRSV in the U.S.A. and Europe. Arch Virol 140:745–755

    Article  PubMed  CAS  Google Scholar 

  13. Meulenberg JJ, Hulst MM, de Meijer EJ, Moonen PJM, den Besten A, De Kluyver EP, Wensvoort G, Moormann RJM (1993) Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192:62–72

    Article  PubMed  CAS  Google Scholar 

  14. Meulenberg JJ, Petersen-den Besten A, De Kluyver EP, Moormann RJ, Schaaper WM, Wensvoort G (1995) Characterization of proteins encoded by ORFs 2 to 7 of Lelystad virus. Virology 206:155–163

    Article  PubMed  CAS  Google Scholar 

  15. Nelsen CJ, Murtaugh MP, Faaberg KS (1999) Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. J Virol 73:270–280

    PubMed  CAS  Google Scholar 

  16. Nelson EA, Christopher-Hennings J, Drew T, Wensvoort G, Collins JE, Benfield DA (1993) Differentiation of US and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies. J Clin Microbiol 31:3184–3189

    PubMed  CAS  Google Scholar 

  17. Nuemann EJ, Kliebenstein JB, Johnson CD, Mabry JW, Bush EJ, Seitzinger AH, Green AL, Zimmerman JJ (2005) Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J Am Vet Med Assoc 227:385–392

    Article  Google Scholar 

  18. Oleksiewicz MB, Botner A, Nielson J, Storgaard T (1999) Determination of the 5′-leader sequences from radically disparate strains of porcine reproductive and respiratory syndrome virus reveals the presence of highly conserved sequence motifs. Arch Virol 144:981–987

    Article  PubMed  CAS  Google Scholar 

  19. Oleksiewicz MB, Botner A, Toft P, Grubbe T, Nielson J, Kamstrup S, Storgaard T (2000) Emergence of porcine reproductive and respiratory syndrome virus deletion mutants. Correlation with the porcine antibody response to a hypervariable site in the ORF3 structural glycoprotein. Virology 267:135–140

    Article  PubMed  CAS  Google Scholar 

  20. Page RDM (1996) Treeview: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  21. Pirzadeh B, Gagnon CA, Dea S (1998) Genetic and antigenic variation of porcine reproductive and respiratory syndrome virus major envelope GP5 glycoprotein. Can J Vet Res 62:170–177

    PubMed  CAS  Google Scholar 

  22. Ropp SL, Mahlum Wees CE, Fang Y, Nelson EA, Rossow KD, Bien M, Arndt B, Preszler S, Steen P, Christopher-Hennings J, Collins JE, Benfield DA, Faaberg KS (2004) Characterization of emerging European-like porcine reproductive and respiratory syndrome virus isolates in the United States. J Virol 78:3684–3703

    Article  PubMed  CAS  Google Scholar 

  23. Sagripanti JL, Zandomeni RO, Weinmann R (1986) The cap structure of simian hemorrhagic fever virion RNA. Virology 151:146–150

    Article  PubMed  CAS  Google Scholar 

  24. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    PubMed  CAS  Google Scholar 

  25. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor

    Google Scholar 

  26. Snijder EJ, Meulenberg JJ (1998) The molecular biology of arteriviruses. J Gen Virol 79:961–979

    PubMed  CAS  Google Scholar 

  27. Stadejek T, Oleksiewicz MB, Scherbakov AV, Timina AM, Krabbe JS, Chabros K, Potapchuk D (2008) Definition of subtypes in the European genotype of porcine reproductive and respiratory syndrome virus: nucleocapsid characteristics and geographical distribution in Europe. Arch Virol 153:1479–1488

    Article  PubMed  CAS  Google Scholar 

  28. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  Google Scholar 

  29. van Dinten LC, Rensen S, Gorbalenya AE, Snijder EJ (1999) Proteolytic processing of the open reading frame 1b-encoded part of arterivirus replicase is mediated by nsp4 serine protease and is essential for virus replication. J Virol 73:2027–2037

    PubMed  Google Scholar 

  30. van Marle G, Dobbe JC, Gultyaev AP, Luytjes W, Spaan WJ, Snijder EJ (1999) Arterivirus discontinuous mRNA transcription is guided by base pairing between sense and antisense transcription-regulating sequences. Proc Natl Acad Sci USA 96:12056–12061

    Article  PubMed  Google Scholar 

  31. Wensvoort G, Tepstra C, Pol JMA, ter Laak EA, Bloemraad M, de Kluyver EP, Kragten C, van Buiten L, den Besten A, Wagenaar F, Broekhuijsen JM, Moonen PLJM, Zestra T, de Boer EA, Tibben HJ, de Jong MF, van Veld P, Groenland GJR, van Gennep JA, Voets MT, Verheijden JHM, Braamskamp J (1991) Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Vet Q 13:121–130

    PubMed  CAS  Google Scholar 

  32. Wootton SK, Yoo D, Rogan D (2000) Full-length sequence of a Canadian porcine reproductive and respiratory syndrome virus (PRRSV) isolate. Arch Virol 145:2297–2323

    Article  PubMed  CAS  Google Scholar 

  33. Wu WH, Fang Y, Farwell R, Steffen-Bien M, Rowland RR, Christopher-Hennings J, Nelson EA (2001) A 10-kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Virology 287:183–191

    Article  PubMed  CAS  Google Scholar 

  34. Yoo D, Welch SK, Lee C, Calvert J (2004) Infectious cDNA clones of porcine reproductive and respiratory syndrome virus and their potential as vaccine vectors. Vet Immunol Immunopathol 102:143–154

    Article  PubMed  CAS  Google Scholar 

  35. Yoon SH, Song JY, Lee CH, Choi EJ, Cho IS, Kim B (2008) Genetic characterization of the Korean porcine reproductive and respiratory syndrome viruses based on the nucleocapsid protein (ORF7) sequences. Arch Virol 153:627–635

    Article  PubMed  CAS  Google Scholar 

  36. Zeman D, Neiger R, Yaeger M, Nelson E, Benfield D, Leslie-steen P, Thomson J, Miskmins D, Daly R, Minehart M (1993) Laboratory investigation of PRRS virus infection in three swine herds. J Vet Diagn Investig 5:522–528

    CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grant no. Z-AD21-2008-08-02 from the National Veterinary Research and Quarantine Service (NVRQS), Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Department of Microbiology, College of Natural Sciences, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Eeuri Nam & Changhee Lee

  2. College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea

    Sang-Geon Yeo

  3. Animal Disease Diagnostic Center, National Veterinary Research and Quarantine Services, Anyang, 430-824, Republic of Korea

    Choi-Kyu Park, Seong-Hee Kim & Yi-Seok Joo

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  1. Eeuri Nam
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  2. Choi-Kyu Park
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  6. Changhee Lee
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Correspondence to Changhee Lee.

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Nam, E., Park, CK., Kim, SH. et al. Complete genomic characterization of a European type 1 porcine reproductive and respiratory syndrome virus isolate in Korea. Arch Virol 154, 629–638 (2009). https://doi.org/10.1007/s00705-009-0347-3

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