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Influence of HBV gene heterogeneity on the failure of immunization with HBV vaccines in eastern China

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Abstract

The influence of hepatitis B virus (HBV) gene heterogeneity on the failure of HBV vaccination in eastern China remains unknown. Here, we assigned 78 hepatitis B surface antigen (HBsAg)-carrier mothers to two groups: 41 mothers from whom transmission of HBV to their children was successfully prevented and 37 mothers whose children were HBsAg positive 1 year after HBV vaccination. The DNA loads in mothers of the failure group (4.17E + 07 copies/ml) were significantly higher than those in the success group (8.40E + 06 copies/ml). However, no difference was found in the S gene mutation rate and genotypes between the groups. Interestingly, Thr123Ala and Gly145Arg were observed only in failure-group mothers, whereas Thr126Asn, Thr126Ser, Thr143Asn, Asp144Gly, and Asp144Ala were seen in the success group. Thus, high viral load is an important risk factor for HBV vaccination failure, which is correlated with the positions of mutations in the S gene, but not with mutant frequencies or genotypes.

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Abbreviations

HBV:

Hepatitis B virus

T123A:

Thr123Ala

T126N:

Thr126Asn

T126S:

Thr126Ser

T143N:

Thr143Asn

D144G:

Asp144Gly

D144A:

Asp144Ala

G145R:

Gly145Arg

Nested-PCR:

Nested-polymerase chain reaction

Aa:

Amino acids

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Acknowledgments

This study was supported by the National Keystone Basic Research Program (973 Program) in China (2007CB513005) and the Science Research Foundation of Medicine in Zhejiang Province, China (2007A064).

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Authors and Affiliations

  1. Department of Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, 310003, Hangzhou, People’s Republic of China

    She-Lan Liu, Min-Wei Li, Jian-er Wo, Li-Wei Lu, Zhen-Juan Chen & Bing Ruan

  2. National Key Laboratory for Diagnosis and Treatment of Infectious Diseases in China, The First Affiliated Hospital, College of Medicine, Zhejiang University, 310003, Hangzhou, People’s Republic of China

    She-Lan Liu, Min-Wei Li, Jian-er Wo, Li-Wei Lu, Zhen-Juan Chen & Bing Ruan

  3. Department of Oncology, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People’s Republic of China

    Ying Dong

  4. Eye Center, The Second Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People’s Republic of China

    Li Zhang

  5. Department of Hepatitis, The Third People’s Hospital, Changzhou, Jiangsu Province, People’s Republic of China

    Yong-Zhong Wang

Authors
  1. She-Lan Liu
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  2. Ying Dong
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  3. Li Zhang
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  8. Yong-Zhong Wang
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  9. Bing Ruan
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Corresponding author

Correspondence to Bing Ruan.

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She-Lan Liu and Ying Dong have contributed equally to this work.

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Liu, SL., Dong, Y., Zhang, L. et al. Influence of HBV gene heterogeneity on the failure of immunization with HBV vaccines in eastern China. Arch Virol 154, 437–443 (2009). https://doi.org/10.1007/s00705-009-0315-y

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  • DOI: https://doi.org/10.1007/s00705-009-0315-y

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