Abstract
The beet necrotic yellow vein virus (BNYVV) RNA-5-encoded p26 protein is involved in the accentuation of symptoms expression of infected Chenopodium quinoa plants and is capable of transcription activation (TA) in yeast. TA was previously localized within the first 55 residues of the p26 protein. Interestingly, TA did not occur when C-terminally deleted forms of p26 were used. We used a genetic screen in the yeast one-hybrid system to select restored TA from randomly generated mutants. The TA domain was found to be located within the first 17 residues. Alanine replacement of aspartic acids 11, 16, and 17 within the full-length p26 prevented TA but did not impair subcellular localization and the symptom expression.
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Acknowledgments
We thank Danièle Scheidecker for all technical assistance, Malek Alioua for sequencing and Kamal Hleibieh for BY-2 cell procedures. L.C. was supported by the Université Louis Pasteur (ULP) under a postdoctoral fellowship. The Inter-Institute Confocal Microscopy Platform used in this study was co-financed by the Région Alsace, the CNRS, the ULP and the Association pour la Recherche sur le Cancer (ARC).
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Covelli, L., Klein, E. & Gilmer, D. The first 17 amino acids of the beet necrotic yellow vein virus RNA-5-encoded p26 protein are sufficient to activate transcription in a yeast one-hybrid system. Arch Virol 154, 347–351 (2009). https://doi.org/10.1007/s00705-008-0306-4
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DOI: https://doi.org/10.1007/s00705-008-0306-4