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Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa

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Abstract

Six isolates of Cassava brown streak virus (CBSV, genus Ipomovirus; Potyviridae) from the Lake Victoria basin in Uganda and Tanzania were characterized. Virus particles were 650 nm long. The complete coat protein (CP)-encoding sequences (1,101 nucleotides, nt) were 90.7–99.5 and 93.7–99.5% identical at the nt and amino acid (aa) levels, respectively. The 3′ untranslated region was 225, 226 or 227 nt long. These eight isolates were only 75.8–77.5% (nt) and 87.0–89.9% (aa) identical when compared to the partial CP sequences (714 nt) of six CBSV isolates characterized previously from the costal lowlands of Tanzania and Mozambique. Hence, two genetically different and geographically separated populations of CSBV exist in East Africa.

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Acknowledgments

We thank Anu Kallinen, Johanna Aura, Marja Calonius, Sascha Kirchner and Peter Wasswa for technical assistance. This study was part of the project “Towards Sustainable Cassava and Sweet Potato Production in Eastern Africa” under the East African Regional Network for Biotechnology, Biosafety and Biotechnology Policy Development programme (BIO-EARN). Funding from Sida, Sweden, and the assistance of the Stockholm Environment Institute (SEI) are gratefully acknowledged.

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Authors and Affiliations

  1. Department of Applied Biology, University of Helsinki, P.O. Box 27, 00014, Helsinki, Finland

    D. R. Mbanzibwa, Y. P. Tian, A. K. Tugume & Jari P. T. Valkonen

  2. Department of Crop Science, Makerere University, P.O. Box 7062, Kampala, Uganda

    D. R. Mbanzibwa, S. B. Mukasa & S. Kyamanywa

  3. Mikocheni Agricultural Research Institute, P.O. Box 6226, Dar es Salaam, Tanzania

    D. R. Mbanzibwa, F. Tairo & A. Kullaya

  4. Department of Botany, Makerere University, P.O. Box 7062, Kampala, Uganda

    A. K. Tugume

Authors
  1. D. R. Mbanzibwa
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  2. Y. P. Tian
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  3. A. K. Tugume
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  4. S. B. Mukasa
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  8. Jari P. T. Valkonen
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Correspondence to Jari P. T. Valkonen.

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Mbanzibwa, D.R., Tian, Y.P., Tugume, A.K. et al. Genetically distinct strains of Cassava brown streak virus in the Lake Victoria basin and the Indian Ocean coastal area of East Africa. Arch Virol 154, 353–359 (2009). https://doi.org/10.1007/s00705-008-0301-9

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  • DOI: https://doi.org/10.1007/s00705-008-0301-9

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