Abstract
Dengue-1 viruses responsible for the dengue fever outbreak in Easter Island in 2002 were isolated from acute-phase sera of dengue fever patients. In order to analyze the complete genome sequence, we designed primers to amplify contiguous segments across the entire sequence of the viral genome. RT-PCR products obtained were cloned, and complete nucleotide and deduced amino acid sequences were determined. This report constitutes the first complete genetic characterization of a DENV-1 isolate from Chile. Phylogenetic analysis shows that an Easter Island isolate is most closely related to Pacific DENV-1 genotype IV viruses.
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Acknowledgments
We thank Maria Ibañez Aravena, from the Public Health Institute of Chile for her excellent technical assistance.
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Cáceres, C., Yung, V., Araya, P. et al. Complete nucleotide sequence analysis of a Dengue-1 virus isolated on Easter Island, Chile. Arch Virol 153, 1967–1970 (2008). https://doi.org/10.1007/s00705-008-0200-0
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DOI: https://doi.org/10.1007/s00705-008-0200-0