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Characterisation of lettuce virus X, a new potexvirus infecting lettuce in Iran

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Abstract

A virus with flexuous rod-shaped particle morphology was found in samples from lettuce during a survey of viruses infecting lettuce in Tehran province in Iran. This virus was subjected to a complete analysis of its biological and molecular features. The entire nucleotide sequence of the virus was determined, revealing a polyadenylated ssRNA genome consisting of 7,212 nucleotides [without poly (A) tail] and possessing an organization typical for potexviruses. Comparative genome analysis showed that the lettuce virus is closely related to Alstroemeria virus X, narcissus mosaic virus and asparagus virus 3. Based on particle morphology, physico-chemical properties and the complete genome sequence, this virus is a member of a new species in the genus Potexvirus, for which the name lettuce virus X (LeVX) is proposed. Biological assays using an infectious cDNA clone and a wild-type isolate of LeVX revealed that the virus, despite reaching high concentrations in all lettuce cultivars tested, does not cause symptoms in lettuce.

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Acknowledgments

This work was supported in part by a scholarship to A. Dizadji from the Ministry of Science, Research and Technology, Iran. We thank Marianne Koerbler and Agnes Pietruszka for excellent technical assistance.

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Authors and Affiliations

  1. Plant Protection Department, Faculty of Horticultural Science and Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    A. Dizadji, M. Koohi-Habibi & G. H. Mossahebi

  2. Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran

    K. Izadpanah

  3. Plant Virus Department, DSMZ, c/o JKI, Braunschweig, Germany

    C. Dietrich & S. Winter

Authors
  1. A. Dizadji
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  2. M. Koohi-Habibi
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  3. K. Izadpanah
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  4. C. Dietrich
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  5. G. H. Mossahebi
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  6. S. Winter
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Correspondence to S. Winter.

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Dizadji, A., Koohi-Habibi, M., Izadpanah, K. et al. Characterisation of lettuce virus X, a new potexvirus infecting lettuce in Iran. Arch Virol 153, 1867–1875 (2008). https://doi.org/10.1007/s00705-008-0191-x

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  • DOI: https://doi.org/10.1007/s00705-008-0191-x

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