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Vimentin is required for dengue virus serotype 2 infection but microtubules are not necessary for this process

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Abstract

The present study investigated the effect of microtubules (MTs) and vimentin during dengue virus serotype 2 (DV2) infection. Immunostaining showed that DV2 infection induced MT and vimentin reorganization. Colocalization of DV2 antigens with MTs or vimentin were often observed in ECV304 cells. MT-disrupting agents could enhance DV2 release but did not affect other steps of virus replication. In contrast, disruption of vimentin inhibited DV2 infection. Our results suggest that an MT-dependent mechanism may not be necessary for DV2 infection, and MT disruption may promote DV2 release. However, vimentin is required for DV2 infection.

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Acknowledgments

This work was supported in parts by grants 30471552, 30640065 and 30671853 from the National Natural Science Foundation of China, and Beijing Natural Science Foundation (5082004).

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Authors and Affiliations

  1. Department of Microbiology, Third Military Medical University, 400038, Chongqing, People’s Republic of China

    Wei Chen, Jia-li Wang & Zong-tao Chen

  2. Department of Histology and Embryology, Third Military Medical University, 400038, Chongqing, People’s Republic of China

    Na Gao & Yan-ping Tian

  3. Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, 100069, Beijing, People’s Republic of China

    Jing An

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  1. Wei Chen
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  2. Na Gao
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  3. Jia-li Wang
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  4. Yan-ping Tian
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  5. Zong-tao Chen
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Correspondence to Jing An.

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Chen, W., Gao, N., Wang, Jl. et al. Vimentin is required for dengue virus serotype 2 infection but microtubules are not necessary for this process. Arch Virol 153, 1777–1781 (2008). https://doi.org/10.1007/s00705-008-0183-x

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  • DOI: https://doi.org/10.1007/s00705-008-0183-x

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