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Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis

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Abstract

A novel cypovirus, assigned CoCPV, was isolated from natural populations of the western spruce budworm, Choristoneura occidentalis. The complete nucleotide sequences of genomic segments S2–S5 and S7–S10 were determined. Each segment contained a single open reading frame. Conserved motifs 5′ (AGUUU……UUUGUGC) 3′ were found at the ends of each segment. Analysis of S2, which encoded a putative RNA-dependent RNA polymerase protein, confirmed CoCPV belonged to the genus Cypovirus within the family Reoviridae. Further phylogenetic analysis using S10 (the polyhedrin gene) aligned this virus with species type-16, closely related to a cypovirus isolated from C. fumiferana.

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Acknowledgments

This study was supported by a grant awarded to CJL from the Canadian Forest Service Canadian Biotechnology Strategic Fund.

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Author notes

  1. Robert I. Graham

    Present address: Department of Biological Sciences, Lancaster University, Lancaster, LA1 4YQ, United Kingdom

Authors and Affiliations

  1. Population Ecology Group, Faculty of Forestry and Environmental Management, University of New Brunswick, Fredericton, NB, Canada, E3B 6C2

    Robert I. Graham & Christopher J. Lucarotti

  2. Natural Resources Canada, Canadian Forest Service-Atlantic Forestry Centre, P.O. Box 4000, Fredericton, NB, Canada, E3B 5P7

    Benoit Morin & Christopher J. Lucarotti

  3. Sylvar Technologies Inc, P.O. Box 636, Stn. “A”, Fredericton, NB, Canada, E3B 5A6

    Renée Lapointe

  4. Natural Resources Canada, Canadian Forest Service-Pacific Forestry Centre, 506 West Burnside Road, Victoria, BC, Canada, V8Z 1M5

    Vincent G. Nealis

Authors
  1. Robert I. Graham
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  2. Benoit Morin
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  3. Renée Lapointe
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  4. Vincent G. Nealis
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  5. Christopher J. Lucarotti
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Corresponding author

Correspondence to Christopher J. Lucarotti.

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Accession numbers

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are EU486988-EU486990 and EU201036-EU201043.

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Graham, R.I., Morin, B., Lapointe, R. et al. Molecular characterisation of a cypovirus isolated from the western spruce budworm Choristoneura occidentalis . Arch Virol 153, 1759–1763 (2008). https://doi.org/10.1007/s00705-008-0175-x

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  • DOI: https://doi.org/10.1007/s00705-008-0175-x

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