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Complete genome analysis of a highly pathogenic H5N1 influenza A virus isolated from a tiger in China

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Abstract

An influenza A virus (A/Tig/SH/01/2005 (H5N1) was isolated from lung tissue samples of a dead zoo tiger with respiratory disease in China in July 2005. Complete genome analysis indicated that the isolate was highly identical to an H5N1 virus isolated from a migratory duck at Poyang lake in China in that year. The genotype of the isolate was K,G,D,5J,F,1J,F,1E, and phylogenetically it was a clade 2.2 virus. Molecular characterization of all of the gene segments revealed characteristics of highly pathogenic influenza A viruses. These results may help to identify molecular determinants of virulence and highlight the necessity for continuous surveillance.

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Acknowledgments

This work was supported by the High-Tech Research and Development Program of China (2006AA10A203), the program for New Century Excellent Talents in University (NCET-04-0502), and partly by grants from the foundation for PhD students training program (20060307007). Muhammad Hassan Mushtaq is supported by HEC Pakistan.

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Authors and Affiliations

  1. Key Laboratory of Animal Diseases Diagnostic and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Ministry of Agriculture, 210095, Nanjing, China

    Muhammad Hassan Mushtaq, Huang Juan, Ping Jiang, Yufeng Li & Yijun Du

  2. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, 430071, Wuhan, China

    TianXian Li

  3. College of Life Sciences, Wuhan University, 430072, Wuhan, People’s Republic of China

    Muhammad Mahmood Mukhtar

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  1. Muhammad Hassan Mushtaq
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  2. Huang Juan
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  3. Ping Jiang
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  4. Yufeng Li
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  5. TianXian Li
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  7. Muhammad Mahmood Mukhtar
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Correspondence to Ping Jiang.

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Mushtaq, M.H., Juan, H., Jiang, P. et al. Complete genome analysis of a highly pathogenic H5N1 influenza A virus isolated from a tiger in China. Arch Virol 153, 1569–1574 (2008). https://doi.org/10.1007/s00705-008-0145-3

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  • DOI: https://doi.org/10.1007/s00705-008-0145-3

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