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Characterization of a pathogenic H9N2 influenza A virus isolated from central China in 2007

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Abstract

The entire genome of the A/Chicken/Hubei/C1/2007 (H9N2) virus, isolated from central China in 2007, was completely sequenced and phylogenetically analyzed. Phylogenetic analysis demonstrated that A/Chicken/Hubei/C1/2007 (H9N2) virus represents multiple reassortant lineages, with genes coming from the early mainland China strain (Ck/Bejing/1/94), an H9N2 virus with special genotype (Ck/shanghai/F/98) and other lineages from poultry in Asia. Infection studies indicated that A/Chicken/Hubei/C1/2007 (H9N2) virus replicated efficiently in MDCK cells and in BALB/c mice. The H9N2 virus also replicated to high titers in chicken respiratory tracts and caused overt clinical signs in chickens. Our results suggest that attention should be paid to the natural evolution of H9N2 influenza viruses and to the control of H9N2 influenza viruses in animals.

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References

  1. Aamir UB, Wernery U, Ilyushina N, Webster RG (2007) Characterization of avian H9N2 influenza viruses from United Arab Emirates 2000 to 2003. Virology 361:45–55

    Article  PubMed  CAS  Google Scholar 

  2. Butt KM, Smith GJ, Chen H, Zhang LJ, Leung YH, Xu KM, Lim W, Webster RG, Yuen KY, Peiris JS, Guan Y (2005) Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003. J Clin Microbiol 43:5760–5767

    Article  PubMed  CAS  Google Scholar 

  3. Capua I, Alexander DJ (2004) Avian influenza: recent developments. Avian Pathol 33:393–404

    Article  PubMed  Google Scholar 

  4. Chen BL, Zhang ZJ, Chen WB (1994) Isolation and preliminary serological characterization of type A influenza viruses from chickens. Chin J Vet Med 22:3–5 (in Chinese)

    Google Scholar 

  5. Choi YK, Ozaki H, Webby RJ, Webster RG, Peiris JS, Poon L, Butt C, Leung YH, Guan Y (2004) Continuing evolution of H9N2 influenza viruses in Southeastern China. J Virol 78:8609–8614

    Article  PubMed  CAS  Google Scholar 

  6. Guan Y, Shortridge KF, Krauss S, Webster RG (1999) Molecular characterization of H9N2 influenza viruses: were they the donors of the “internal” genes of H5N1 viruses in Hong Kong? Proc Natl Acad Sci USA 96:9363–9367

    Article  PubMed  CAS  Google Scholar 

  7. Guan Y, Shortridge KF, Krauss S, Chin PS, Dyrting KC, Ellis TM, Webster RG, Peiris M (2000) H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China. J Virol 74:9372–9380

    Article  PubMed  CAS  Google Scholar 

  8. Guo YJ, Li J, Cheng X, Wang M, Zhou Y, Li C, Cai F, Liao H, Zhang Y, Guo J, Huang R, Bei D (1999) Discovery of men infected by avian influenza A(H9N2) virus. Chin J Exp Clin Virol 13:105–108

    CAS  Google Scholar 

  9. Guo YJ, Xie J, Wang M, Dong J, Guo J, Zhang Y, Wu K (2000) A strain of influenza A H9N2 virus repeatedly isolated from human population in China. Chin J Exp Clin Virol 14:209–212

    CAS  Google Scholar 

  10. Guo YJ, Krauss S, Senne DA, Mo IP, Lo KS, Xiong XP, Norwood M, Shortridge KF, Webster RG, Guan Y (2000) Characterization of the pathogenicity of members of the newly established H9N2 influenza virus lineages in Asia. Virology 267:279–288

    Article  PubMed  CAS  Google Scholar 

  11. Hoffmann E, Stech J, Guan Y, Webster RG, Perez DR (2001) Universal primer set for the full-length amplification of all influenza A viruses. Arch Virol 146:2275–2289

    Article  PubMed  CAS  Google Scholar 

  12. Kim JA, Cho SH, Kim HS, Seo SH (2006) H9N2 influenza viruses isolated from poultry in Korean live bird markets continuously evolve and cause the severe clinical signs in layers. Vet Microbiol 118:169–176

    Article  PubMed  CAS  Google Scholar 

  13. Lee YJ, Shin JY, Song MS, Lee YM, Choi JG, Lee EK, Jeong OM, Sung HW, Kim JH, Kwon YK, Kwon JH, Kim CJ, Webby RJ, Webster RG, Choi YK (2007) Continuing evolution of H9 influenza viruses in Korean poultry. Virology 359:313–323

    Article  PubMed  CAS  Google Scholar 

  14. Lu JH, Liu XF, Shao WX, Liu YL, Wei DP, Liu HQ (2005) Phylogenetic analysis of eight genes of H9N2 subtype influenza virus: a mainland China strain possessing early isolates’ genes that have been circulating. Virus Genes 31:163–169

    Article  PubMed  CAS  Google Scholar 

  15. Matrosovich MN, Krauss S, Webster RG (2001) H9N2 influenza A viruses from poultry in Asia have human virus-like receptor specificity. Virology 281:156–162

    Article  PubMed  CAS  Google Scholar 

  16. Nili H, Asasi K (2002) Natural cases and an experimental study of H9N2 avian influenza in commercial broiler chickens of Iran. Avian Pathol 31:247–252

    Article  PubMed  Google Scholar 

  17. Peiris M, Yuen KY, Leung CW, Chan KH, Ip PL, Lai RW, Orr WK, Shortridge KF (1999) Human infection with influenza H9N2. Lancet 354:916–917

    Article  PubMed  CAS  Google Scholar 

  18. Subbarao K, Katz J (2000) Avian influenza viruses infecting humans. Cell Mol Life Sci 57:1770–1784

    Article  PubMed  CAS  Google Scholar 

  19. Xu C, Fan W, Wei R, Zhao H (2004) Isolation and identification of swine influenza recombinant A/Swine/Shandong/1/2003(H9N2) virus. Microbes Infect 6:919–925

    Article  PubMed  CAS  Google Scholar 

  20. Xu KM, Li KS, Smith GJ, Li JW, Tai H, Zhang JX, Webster RG, Peiris JS, Chen H, Guan Y (2007) Evolution and molecular epidemiology of H9N2 influenza A viruses from quail in southern China, 2000 to 2005. J Virol 81:2635–2645

    Article  PubMed  CAS  Google Scholar 

  21. Xu KM, Smith GJ, Bahl J, Duan L, Tai H, Vijaykrishna D, Wang J, Zhang JX, Li KS, Fan XH, Webster RG, Chen H, Peiris JS, Guan Y (2007) The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005. J Virol 81:10389–10401

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This study was supported by grant NO. I-2005011 from The Basic Research Program of the State Key Laboratory of Virology, China.

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Authors and Affiliations

  1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Hubei, Wuhan, 430071, China

    R. Wu, Z. W. Sui, H. B. Zhang, Q. J. Chen & Z. Chen

  2. Graduate University of Chinese Academy of Sciences, Beijing, China

    R. Wu

  3. Institute of Animal Husbandry and Veterinary Science, Hubei Academy of Agricultural Sciences, Hubei, Wuhan, 430064, China

    R. Wu, W. W. Liang, K. L. Yang, Z. L. Xiong, Z. W. Liu & D. P. Xu

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  1. R. Wu
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  2. Z. W. Sui
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  3. H. B. Zhang
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  4. Q. J. Chen
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  5. W. W. Liang
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  7. Z. L. Xiong
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  8. Z. W. Liu
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  9. Z. Chen
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  10. D. P. Xu
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Correspondence to Z. Chen or D. P. Xu.

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Wu, R., Sui, Z.W., Zhang, H.B. et al. Characterization of a pathogenic H9N2 influenza A virus isolated from central China in 2007. Arch Virol 153, 1549–1555 (2008). https://doi.org/10.1007/s00705-008-0139-1

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  • DOI: https://doi.org/10.1007/s00705-008-0139-1

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