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Satellite DNA β overrides the pathogenicity phenotype of the C4 gene of tomato leaf curl virus but does not compensate for loss of function of the coat protein and V2 genes

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Abstract

We have investigated the ability of satellite DNA β to complement mutations in the CP, V2 and C4 genes of the monopartite begomovirus, tomato leaf curl virus, which are potentially involved in movement. A mutation in the coat protein was not complemented by DNA β. Mutations of the C4 and V2 genes attenuated and abolished symptoms, respectively. In the presence of the C4 mutant, but not the V2 mutant, DNA β induced typical symptoms, confirming that the satellite encodes a dominant symptom determinant. In contrast to the C4 mutant, DNA β did not enhance the viral DNA levels of the V2 mutant, suggesting that V2 is required for this phenomenon. The significance of these findings is discussed based on our present understanding of the functions of the viral genes and DNA β.

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Acknowledgments

We thank Kausar Malik and Yusuf Zafar for their support throughout the project. This project was funded by the Australian Centre for International Agricultural Research (ACIAR). MS thanks ACIAR and the Higher Education Commission HEC, Pakistan for financial support. RWB is employed at NIBGE under the HEC “Foreign Faculty Hiring Scheme.”

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Authors and Affiliations

  1. National Institute for Biotechnology and Genetic Engineering, PO Box 577, Jhang Road, Faisalabad, Pakistan

    M. Saeed, S. Mansoor & R. W. Briddon

  2. School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA, 5064, Australia

    M. Saeed & J. W. Randles

  3. CSIRO Plant Industry, PO Box 350, Glen Osmond, SA, 5064, Australia

    M. Saeed & M. A. Rezaian

Authors
  1. M. Saeed
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  2. S. Mansoor
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  3. M. A. Rezaian
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  4. R. W. Briddon
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  5. J. W. Randles
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Correspondence to M. Saeed.

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Saeed, M., Mansoor, S., Rezaian, M.A. et al. Satellite DNA β overrides the pathogenicity phenotype of the C4 gene of tomato leaf curl virus but does not compensate for loss of function of the coat protein and V2 genes. Arch Virol 153, 1367–1372 (2008). https://doi.org/10.1007/s00705-008-0124-8

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  • DOI: https://doi.org/10.1007/s00705-008-0124-8

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