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The genomic sequence and biological properties of Pennisetum mosaic virus, a novel monocot-infecting potyvirus

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Abstract

The complete nucleotide sequences of two isolates of Pennisetum mosaic virus (PenMV) were determined. The viral genome comprised 9,611 nucleotides (nt) excluding the 3′-terminal poly(A) sequence, with the capacity of encoding a single polyprotein of 3,065 amino acids. The large open reading frame is flanked by a 172-nt 5′-untranslated region (UTR) and a 244-nt 3′-UTR. Sequence comparisons and phylogenetic analyses of the complete genome and polyproteins suggest that PenMV is closely related to other monocot potyviruses such as Maize dwarf mosaic virus, Sorghum mosaic virus and Sugarcane mosaic virus (SCMV), and thus represents a distinct potyvirus within the SCMV subgroup. The host range of PenMV is limited to Gramineae, and the virus naturally infects maize, sorghum and some wild grasses, causing mosaic symptoms on the leaves. This virus could be transmitted by both mechanical inoculation and by at least four species of aphids.

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References

  1. Achon MA, Serrano L, Alonso-Duenas N, Porta C (2007) Complete genome sequences of Maize dwarf mosaic and Sugarcane mosaic virus isolates coinfecting maize in Spain. Arch Virol 152:2073–2078

    Article  PubMed  CAS  Google Scholar 

  2. Adams MJ, Antoniw JF, Beaudoin F (2005) Overview and analysis of the polyprotein cleavage sites in the family Potyviridae. Mol Plant Pathol 6:471–487

    Article  CAS  Google Scholar 

  3. Adams MJ, Antoniw JF, Fauquet CM (2005) Molecular criteria for genus and species discrimination within the family Potyviridae. Arch Virol 150:459–479

    Article  PubMed  CAS  Google Scholar 

  4. Berger PH, Adams MJ, Barnett OW, Brunt AA, Hammond J, Hill JH, Jordan RL, Kashiwazaki S, Rybicki E, Spence N, Stenger DC, Ohki ST, Uyeda I, van Zaayen A, Valkonen J, Vetten HJ (2005) Potyviridae. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy, 8th report of the ICTV. Elsevier Academic Press, San Diego, pp 819–841

    Google Scholar 

  5. Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–83

    Article  PubMed  CAS  Google Scholar 

  6. Desbiez C, Lecoq H (2004) The nucleotide sequence of Watermelon mosaic virus (WMV, Potyvirus) reveals interspecific recombination between two related potyviruses in the 5′ part of the genome. Arch Virol 149:1619–1632

    Article  PubMed  CAS  Google Scholar 

  7. Fan ZF, Chen HY, Cai SS, Deng CL, Wang WJ, Liang XM, Li HF (2003) Molecular characterization of a distinct potyvirus from whitegrass in China. Arch Virol 148:1219–1224

    Article  PubMed  CAS  Google Scholar 

  8. Fan ZF, Chen HY, Liang XM, Li HF (2003) Complete sequence of the genomic RNA of the prevalent strain of a potyvirus infecting maize in China. Arch Virol 148:773–782

    Article  PubMed  CAS  Google Scholar 

  9. Fan ZF, Deng CL, Chen HY, Cai SS, Wang WJ, Liang XM, Jiang X, Li HF (2004) Molecular variation in Pennisetum mosaic virus. Phytopathology 94:S29

    Google Scholar 

  10. Fan ZF, Wang WJ, Jiang X, Liang XM, Wang FR, Li HF (2004). Natural infection of maize by Pennisetum mosaic virus in China. Plant Pathol 53:796

    Article  Google Scholar 

  11. Ivanov KI, Puustinen P, Gabrenaite R, Vihinen H, Ronnstrand L, Valmu L, Kalkkinen N, Makinen K (2003) Phosphorylation of the potyvirus capsid protein by protein kinase CK2 and its relevance for virus infection. Plant Cell 15:2124–2139

    Article  PubMed  CAS  Google Scholar 

  12. Liang WX, Song LM, Tian GZ, Li HF, Fan ZF (2006) The genomic sequence of Wisteria vein mosaic virus and its relations to other potyviruses. Arch Virol 151:2311–2319

    Article  PubMed  CAS  Google Scholar 

  13. Lütcke HA, Chow KC, Mickel FS, Moss KA, Kern HF, Scheele GA (1987) Selection of AUG initiation codons differs in plants and animals. EMBO J 6:43–48

    PubMed  Google Scholar 

  14. Meggio F, Pinna LA (2003) One-thousand-and one substrates of protein kinase CK2? FASEB J 17:349–368

    Article  PubMed  CAS  Google Scholar 

  15. Revers F, Le Gall O, Candresse T, Maule AJ (1999) New advances in understanding the molecular biology of plant/potyvirus interactions. Mol Plant Microbe Interact 12:367–376

    Article  CAS  Google Scholar 

  16. Shukla DD, Ward CW, Brunt AA (1994) The potyviridae. CAB International, Wallingford

    Google Scholar 

  17. Urcuqui-Inchima S, Haenni AL, Bernadi F (2001) Potyvirus proteins: a wealth of functions. Virus Res 74:157–175

    Article  PubMed  CAS  Google Scholar 

  18. Zhong Y, Guo A, Li CH, Zhuang B, Lai M, Wei CH, Luo J, Li Y (2005) Identification of a naturally occurring recombination isolate of sugarcane mosaic virus causing maize dwarf mosaic disease. Virus Genes 30:75–83

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by the National Key Basic Research Program of China (2006CB101903) and the National Natural Science Foundation of China (#30571213 and #30170608). We thank Prof. Yinlu Shi from Shanxi Academy of Agricultural Sciences for providing various plant materials used in the present research and the two anonymous reviewers for their objective comments and helpful suggestions for the modification of our manuscript.

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  1. C. L. Deng

    Present address: Plant Quarantine Laboratory, Beijing Exit–Entry Inspection and Quarantine Bureau, 100026, Beijing, China

Authors and Affiliations

  1. Department of Plant Pathology and State Key Laboratory of Agro-biotechnology, China Agricultural University, 100094, Beijing, China

    C. L. Deng, W. J. Wang, Z. Y. Wang, X. Jiang, Y. Y. Cao, T. Zhou, H. F. Li & Z. F. Fan

  2. Maize Research Institute, Shanxi Academy of Agricultural Sciences, Xinzhou, 034000, Shanxi, China

    W. J. Wang & F. R. Wang

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  1. C. L. Deng
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  2. W. J. Wang
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  4. X. Jiang
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  5. Y. Y. Cao
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Correspondence to Z. F. Fan.

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Deng, C.L., Wang, W.J., Wang, Z.Y. et al. The genomic sequence and biological properties of Pennisetum mosaic virus, a novel monocot-infecting potyvirus. Arch Virol 153, 921–927 (2008). https://doi.org/10.1007/s00705-008-0068-z

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  • DOI: https://doi.org/10.1007/s00705-008-0068-z

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