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Coat protein gene diversity among Chrysanthemum virus B isolates from India

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Summary.

The complete coat protein (CP) sequences from 29 Indian isolates of Chrysanthemum virus B (CVB) were determined and analysed in relation to other previously characterized carlaviruses. The CP genes of the Indian CVB isolates were highly heterogeneous, sharing nucleotide sequence identities of 74–98%. Based on phylogenetic analyses, the isolates formed three groups potentially representing either two or three major CVB strain groupings. Recombination analysis revealed at least one definite recombination event involving the exchange of sequences between members of different groups. To our knowledge this is the first reported evidence of homologous recombination in carlaviruses.

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References

  • OM Alegria M Royer M Bousalem M Chatenet M Peterschmitt JC Girard P Rott (2003) ArticleTitleGenetic diversity in the coat protein region of sugarcane mosaic virus isolates from eight countries, particularly from Cameroon and Congo Arch Virol 148 357–372 Occurrence Handle12556998 Occurrence Handle10.1007/s00705-002-0916-1 Occurrence Handle1:CAS:528:DC%2BD3sXntV2gtA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • SF Altschul W Gish W Miller EW Myers DJ Lipman (1990) ArticleTitleBasic local alignment search tool J Mol Biol 215 403–410 Occurrence Handle2231712 Occurrence Handle10.1006/jmbi.1990.9999 Occurrence Handle1:CAS:528:DyaK3MXitVGmsA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • K Bananej A Kheyr-Pour G Hosseini-Salekdeh G Ahoonmanesh (2004) ArticleTitleComplete nucleotide sequence of Iranian isolate of Tomato yellow leaf curl virus: further evidence for natural recombination among geminiviruses Arch Virol 149 1435–1443 Occurrence Handle15221543 Occurrence Handle10.1007/s00705-004-0308-9 Occurrence Handle1:CAS:528:DC%2BD2cXltVKms7Y%3D

    Article  PubMed  CAS  Google Scholar 

  • ER Chare EC Holmes (2006) ArticleTitleA phylogenetic survey of recombination frequency in plant RNA viruses Arch Virol 151 933–946 Occurrence Handle16292597 Occurrence Handle10.1007/s00705-005-0675-x Occurrence Handle1:CAS:528:DC%2BD28Xjs1Klurw%3D

    Article  PubMed  CAS  Google Scholar 

  • R Chenna H Sugawara T Koike R Lopez TJ Gibson DG Higgins JD Thompson (2003) ArticleTitleMultiple sequence alignment with the Clustal series of programs Nucleic Acids Res 31 3497–3500 Occurrence Handle12824352 Occurrence Handle10.1093/nar/gkg500 Occurrence Handle1:CAS:528:DC%2BD3sXltVWjsrg%3D

    Article  PubMed  CAS  Google Scholar 

  • SA Choi KH Ryu (2003) ArticleTitleThe complete nucleotide sequence of the genome RNA of Lily symptomless virus and its comparison with that of other carlaviruses Arch Virol 148 1943–1955 Occurrence Handle14551817 Occurrence Handle10.1007/s00705-003-0155-0 Occurrence Handle1:CAS:528:DC%2BD3sXnvFalt7k%3D

    Article  PubMed  CAS  Google Scholar 

  • J Felsenstein (1989) ArticleTitlePHYLIP – Phylogeny inference package (version 3.2) Cladistics 5 164–166

    Google Scholar 

  • R Froissart D Roze M Uzest L Galibert S Blanc Y Michalakis (2005) ArticleTitleRecombination every day: abundant recombination in a virus during a single multi-cellular host infection PloS Biol 3 e89 Occurrence Handle15737066 Occurrence Handle10.1371/journal.pbio.0030089 Occurrence Handle1:CAS:528:DC%2BD2MXisVyls7g%3D

    Article  PubMed  CAS  Google Scholar 

  • MJ Gibbs JS Armstrong AJ Gibbs (2000) ArticleTitleSister-scanning: a Monte Carlo procedure for assessing signals in recombinant sequences Bioinformatics 16 573–582 Occurrence Handle11038328 Occurrence Handle10.1093/bioinformatics/16.7.573 Occurrence Handle1:CAS:528:DC%2BD3cXosFSqs7g%3D

    Article  PubMed  CAS  Google Scholar 

  • S Guindon O Gascuel (2003) ArticleTitleA simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood Syst Biol 52 696–704 Occurrence Handle14530136 Occurrence Handle10.1080/10635150390235520

    Article  PubMed  Google Scholar 

  • I Haselhof RH Symons (1981) ArticleTitleChrysanthemum stunt viroid: primary sequence and secondary structure Nucleic Acids Res 9 2741–2752 Occurrence Handle10.1093/nar/9.12.2741

    Article  Google Scholar 

  • Koenig R (1982) Carlavirus group. CMI/AAB Description of plant viruses no. 259

  • KE Levay SK Zavriev (1991) ArticleTitleNucleotide sequence and gene organisation of the 3′-terminal region of Chrysanthemum virus B genomic RNA J Gen Virol 72 2333–2337 Occurrence Handle1919520 Occurrence Handle10.1099/0022-1317-72-10-2333

    Article  PubMed  Google Scholar 

  • J Martelik V Mokra (1998) ArticleTitleTomato spotted wilt virus in ornamental plants, vegetables and weeds in the Czech Republic Acta Virol 42 347–351

    Google Scholar 

  • DP Martin D Posada KA Crandall C Williamson (2005) ArticleTitleA modified bootscan algorithm for automated identification of recombinant sequences and recombination breakpoints AIDS Res Hum Retrovir 21 98–102 Occurrence Handle15665649 Occurrence Handle10.1089/aid.2005.21.98 Occurrence Handle1:CAS:528:DC%2BD2MXmtlOntA%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • DP Martin E Rybicki (2000) ArticleTitleRDP: detection of recombination amongst aligned sequences Bioinformatics 16 562–563 Occurrence Handle10980155 Occurrence Handle10.1093/bioinformatics/16.6.562 Occurrence Handle1:CAS:528:DC%2BD3cXnsVelsbc%3D

    Article  PubMed  CAS  Google Scholar 

  • DP Martin C Williamson D Posada (2005) ArticleTitleRDP2: Recombination detection and analysis from sequence alignments Bioinformatics 21 260–262 Occurrence Handle15377507 Occurrence Handle10.1093/bioinformatics/bth490 Occurrence Handle1:CAS:528:DC%2BD2MXkt1Whug%3D%3D

    Article  PubMed  CAS  Google Scholar 

  • J Maynard Smith (1992) ArticleTitleAnalysing the mosaic structure of genes J Mol Evol 34 126–129

    Google Scholar 

  • J Navas-Castillo S Sanchez-Camposs E Noris D Louro GP Accotto E Moriones (2000) ArticleTitleNatural recombination between Tomato yellow leaf curl virus-Is and Tomato leaf curl virus J Gen Virol 81 2797–2801 Occurrence Handle11038394 Occurrence Handle1:CAS:528:DC%2BD3cXnvFWnsr4%3D

    PubMed  CAS  Google Scholar 

  • D O’Reilly CJ Thomas RHA Coutts (1991) ArticleTitleTomato aspermy virus has an evolutionary relationship with other tripartite RNA viruses J Gen Virol 72 1–7 Occurrence Handle1990057 Occurrence Handle1:CAS:528:DyaK3MXks1Glsr0%3D

    PubMed  CAS  Google Scholar 

  • K Ohshima Y Yamaguchi R Hirota T Hamamoto K Tomimura Z Tan T Sano F Azuhata JA Walsh J Fletcher J Chen A Gera A Gibbs (2002) ArticleTitleMolecular evolution of Turnip mosaic virus: evidence of host adaptation, genetic recombination and geographical spread J Gen Virol 83 1511–1521 Occurrence Handle12029167 Occurrence Handle1:CAS:528:DC%2BD38XktlGitb0%3D

    PubMed  CAS  Google Scholar 

  • M Padidam S Sawyer C Fauquet (1999) ArticleTitlePossible emergence of new geminiviruses by frequent recombination Virology 265 218–225 Occurrence Handle10600594 Occurrence Handle10.1006/viro.1999.0056 Occurrence Handle1:CAS:528:DyaK1MXnvFertbs%3D

    Article  PubMed  CAS  Google Scholar 

  • RK Raizada KM Srivastava G Chandra BP Singh (1989) ArticleTitleComparative evaluation of sero-diagnostic methods for detection of Chrysanthemum virus B in chrysanthemum Indian J Exp Biol 27 1094–1096

    Google Scholar 

  • CP Romaine RK Horst (1975) ArticleTitleSuggested viroid etiology of Chrysanthemum chlorotic mottle disease Virology 64 86–95 Occurrence Handle234655 Occurrence Handle10.1016/0042-6822(75)90081-1 Occurrence Handle1:STN:280:CSqC3cvjtVQ%3D

    Article  PubMed  CAS  Google Scholar 

  • MJ Roossinck (1997) ArticleTitleMechanisms of plant virus evolution Annu Rev Phytopathol 35 191–209 Occurrence Handle15012521 Occurrence Handle10.1146/annurev.phyto.35.1.191 Occurrence Handle1:CAS:528:DyaK2sXmtVGhsro%3D

    Article  PubMed  CAS  Google Scholar 

  • L Rubio MA Ayllon P Kong A Fernandez M Polek J Guerri P Moreno BW Falk (2001) ArticleTitleGenetic variation of Citrus tristeza virus isolates from California and Spain: evidence for mixed infections and recombination J Virol 75 8054–8062 Occurrence Handle11483750 Occurrence Handle10.1128/JVI.75.17.8054-8062.2001 Occurrence Handle1:CAS:528:DC%2BD3MXmtFejtb4%3D

    Article  PubMed  CAS  Google Scholar 

  • AE Simon JJ Bujarski (1994) ArticleTitleRNA-RNA recombination and evolution in virus-infected plants Annu Rev Phytopathol 29 337–361 Occurrence Handle10.1146/annurev.py.32.090194.002005

    Article  Google Scholar 

  • Singh HP (2000) Current status of floriculture – national and international scenario. In: Singh HP, Dadlani NK (eds) Commercial floriculture. FAO and DAC, Ministry of Agriculture, Govt. of India, New Delhi, pp 1–26

  • GR Smith Z Borg BEL Lockhart KS Braithwaite MJ Gibbs (2000) ArticleTitleSugarcane yellow leaf virus: a novel member of the Luteoviridae that probably arose by inter-species recombination J Gen Virol 81 1865–1869 Occurrence Handle10859394 Occurrence Handle1:CAS:528:DC%2BD3cXks12hs7w%3D

    PubMed  CAS  Google Scholar 

  • JH Strauss EG Strauss (1988) ArticleTitleEvolution of RNA viruses Annu Rev Microbiol 42 657–683 Occurrence Handle3060004 Occurrence Handle10.1146/annurev.mi.42.100188.003301 Occurrence Handle1:CAS:528:DyaL1cXmt1ent7w%3D

    Article  PubMed  CAS  Google Scholar 

  • Z Tan Y Wada J Chen K Ohshima (2004) ArticleTitleInter- and intralineage recombinants are common in natural populations of Turnip mosaic virus J Gen Virol 85 2683–2696 Occurrence Handle15302962 Occurrence Handle10.1099/vir.0.80124-0 Occurrence Handle1:CAS:528:DC%2BD2cXnsVegs7c%3D

    Article  PubMed  CAS  Google Scholar 

  • JTJ Verhoeven JW Roenhorst I Cortes D Peters (1996) ArticleTitleDetection of a novel tospovirus in chrysanthemum Acta Hort 432 44–51

    Google Scholar 

  • N Verma A Sharma R Ram V Hallan AA Zaidi ID Garg (2003) ArticleTitleDetection, identification and incidence of Chrysanthemum B carlavirus in chrysanthemum in India Crop Prot 22 425–429 Occurrence Handle10.1016/S0261-2194(02)00184-9

    Article  Google Scholar 

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Authors and Affiliations

  1. Plant Virus Laboratory, Institute of Himalayan Bioresource Technology, Palampur, India

    L. Singh, V. Hallan, N. Jabeen, A. K. Singh, R. Ram & A. A. Zaidi

  2. Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Cape Town, South Africa

    D. P. Martin

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  1. L. Singh
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  2. V. Hallan
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  3. N. Jabeen
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  4. A. K. Singh
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  5. R. Ram
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  6. D. P. Martin
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  7. A. A. Zaidi
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Singh, L., Hallan, V., Jabeen, N. et al. Coat protein gene diversity among Chrysanthemum virus B isolates from India. Arch Virol 152, 405–413 (2007). https://doi.org/10.1007/s00705-006-0854-4

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