Skip to main content

Advertisement

SpringerLink
Log in

Cytomegalovirus antibodies in tear fluid of patients with retinitis

  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary.

The diagnosis of cytomegalovirus retinitis (CMV-R) is difficult and usually based on clinical criteria or invasive diagnostic procedures. The purpose of this study was to investigate a possible association between CMV-R and specific anti-CMV antibodies in tears. Paired tear and serum samples were obtained from 96 individuals, which included 20 children with congenital CMV infection and chorioretinitis, 56 adults with retinitis with clinical signs suggestive of viral infection, and 20 healthy control subjects, and were tested for CMV antibodies using ELISA. The prevalence of anti-CMV antibodies in tears was found to be 80% (16/20) in children, 35% (20/56) in adults, and 5% (1/20) in control subjects. Furthermore, high antibody levels were detected in 35% (7/20) of children and 10.7% (6/56) of adults with retinitis, and were not found in control subjects. There was a strong association between high tear levels of anti-CMV antibodies and active ocular infection. No correlations were found between tear and serum antibodies. ELISA sensitivity was 80% and specificity 95%. Further studies are needed to compare the tear and intraocular levels of CMV-specific antibodies in patients with retinitis to find out if CMV antibody testing in tear fluid could substitute for more invasive diagnostic procedures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • T Abe K Tsuchida M Tamai (1996) ArticleTitleA comparative study of the polymerase chain reaction and local antibody production in acute retinal necrosis syndrome and Cytomegalovirus retinitis Graefes Arch. Clin Exp Ophthalmol 234 419–424 Occurrence Handle8817284 Occurrence Handle1:STN:280:DyaK28vhsVamtA%3D%3D

    PubMed  CAS  Google Scholar 

  • S Boppana C Amos W Britt S Stango C Alford R Pass (1994) ArticleTitleLate onset and reactivation of chorioretinitis in children with congenital Cytomegalovirus infection Pediatr Infect J 13 1139–1142 Occurrence Handle1:STN:280:DyaK2M7pvFWhtQ%3D%3D Occurrence Handle10.1097/00006454-199412000-00012

    Article  CAS  Google Scholar 

  • HB Chawla MJ Ford JF Munro RE Scorgie AR Watson (1976) ArticleTitleOcular involvement in Cytomegalovirus infection in a previously healthy adult Br Med J 31 281–282 Occurrence Handle10.1136/bmj.2.6030.281

    Article  Google Scholar 

  • DK Coats GJ Demler EA Paysee LT Du C Libby (2000) ArticleTitleOphthalmologic findings in children with congenital Cytomegalovirus infection JAAPOS 4 110–116 Occurrence Handle1:STN:280:DC%2BD3c3jvVWmtw%3D%3D

    CAS  Google Scholar 

  • F Cox D Meyer WT Hughes (1975) ArticleTitleCytomegalovirus in tears from patients with normal eyes and with acute Cytomegalovirus chorioretinitis Am J Ophthalmol 80 817–824 Occurrence Handle171959 Occurrence Handle1:STN:280:DyaE28%2FlvFertQ%3D%3D

    PubMed  CAS  Google Scholar 

  • PK Coyle PA Sibony (1986) ArticleTitleTear immunoglobulins measured by ELISA Invest Ophthalmol Vis Sci 27 622–625 Occurrence Handle3957581 Occurrence Handle1:CAS:528:DyaL28Xit1ejsrY%3D

    PubMed  CAS  Google Scholar 

  • PK Coyle PA Sibony (1988) ArticleTitleViral antibodies in normal tears Invest Ophthalmol Vis Sci 29 1552–1558 Occurrence Handle3170127 Occurrence Handle1:STN:280:DyaL1M%2FgslCjtA%3D%3D

    PubMed  CAS  Google Scholar 

  • ET Cunningham JS Pepose GN Holland (2001) Cytomegalovirus infection of the retina SJ Ryan (Eds) Retina EditionNumber3 Mosby London 1558–1575

    Google Scholar 

  • J Denis (1976) ArticleTitleImmunological study of the aqueous humor in ocular herpes simplex Med Probl Ophthalmol 16 220–224 Occurrence Handle1:STN:280:DyaE287isFOqsQ%3D%3D

    CAS  Google Scholar 

  • JL Davis W Feuer WW Culbertson SC Pfugfelder (1995) ArticleTitleInterpretation of intraocular and serum antibody levels in necrotizing retinitis Retina 15 233–240 Occurrence Handle7569351 Occurrence Handle1:STN:280:DyaK28%2FjsFWjtQ%3D%3D Occurrence Handle10.1097/00006982-199515030-00008

    Article  PubMed  CAS  Google Scholar 

  • JH De Boer L Luyendijk A Rothova GS Baarsma PTVM de Jong J-G Bollemeijer AJJM Rademakers A Van der Lelij MJW Zaal A Kijlstra (1994) ArticleTitleDetection of intraocular antibody production to Herpesviruses in acute retinal necrosis syndrome Am J Ophthalmol 117 201–210 Occurrence Handle8116748 Occurrence Handle1:STN:280:DyaK2c7mtFWnsg%3D%3D

    PubMed  CAS  Google Scholar 

  • JH De Boer C Verhagen M Bruinenberg A Rothova PTVM de Jong GS Baarsma A Van der Lelij FM Ooyman J-G Bollemeijer PJFM Derhaag A Kijlstra (1996) ArticleTitleSerologic and polymerase chain reaction analysis of intraocular fluids in the diagnosis of infectious uveitis Am J Ophthalmol 121 650–658 Occurrence Handle8644808 Occurrence Handle1:STN:280:DyaK283ktlKmuw%3D%3D

    PubMed  CAS  Google Scholar 

  • P Doornenbal GS Baarsma WG Quint A Kijlstra PH Rothbarth HG Niesters (1996) ArticleTitleDiagnostic assay in Cytomegalovirus retinitis: detection of Herpesvirus by simultaneous application of the polymerase chain reaction and local antibody analysis on ocular fluid Br J Ophthalmol 80 195–196

    Google Scholar 

  • WL Drew (1992) ArticleTitleCytomegalovirus infection in patients with AIDS Clin Infect Dis 14 608–615 Occurrence Handle1313313 Occurrence Handle1:STN:280:DyaK383gvVWnug%3D%3D

    PubMed  CAS  Google Scholar 

  • PD Fox PT Khaw BW McBride JI McGill KA Ward (1986) ArticleTitleTear and serum antibody levels in ocular herpetic infection: diagnostic precision of secretory IgA Br J Ophthalmol 70 584–588 Occurrence Handle3741822 Occurrence Handle1:STN:280:DyaL283ps1GrtQ%3D%3D

    PubMed  CAS  Google Scholar 

  • CK Gupta J Leszczynski RK Gupta GR Siber (1996) ArticleTitleAn enzyme immunoassay based micro-neutralization test for titration of antibodies to human cytomegalovirus (CMV) and its correlation with direct ELISA measuring CMV IgG antibodies Biologicals 24 41–49 Occurrence Handle8733600 Occurrence Handle1:CAS:528:DyaK28XjsFSqsrs%3D Occurrence Handle10.1006/biol.1996.0004

    Article  PubMed  CAS  Google Scholar 

  • DA Jabs ML Van Natta JE Thorne DV Weinberg TA Meredith BD Kuppermann K Sepkowitz HK Li (2004) ArticleTitleCourse of Cytomegalovirus retinitis in the era of highly active antiretroviral therapy. 1. Retinitis progression Ophthalmology 111 2224–2231 Occurrence Handle15582078 Occurrence Handle10.1016/j.ophtha.2004.05.031

    Article  PubMed  Google Scholar 

  • HE Kaufman AM Azcuy ED Varnell GD Sloop HW Thompson JM Hill (2005) ArticleTitleHSV-1 DNA in tears and saliva of normal adults Invest Ophthalmol Vis Sci 46 241–247 Occurrence Handle15623779 Occurrence Handle10.1167/iovs.04-0614

    Article  PubMed  Google Scholar 

  • CM Knox D Chandler GA Short TP Margolis (1997) ArticleTitlePolymerase chain reaction-based assays of vitreous samples for the diagnosis of viral retinitis. Use in diagnostic dilemmas Ophthalmology 105 37–44 Occurrence Handle10.1016/S0161-6420(98)71127-2

    Article  Google Scholar 

  • IC Kuo JH Kempen JP Dunn G Vogelsang DA Jabs (2004) ArticleTitleClinical characteristics and outcomes of Cytomegalovirus retinitis in persons without human immunodeficiency virus infection Am J Ophthalmol 138 338–346 Occurrence Handle15364214 Occurrence Handle10.1016/j.ajo.2004.04.015

    Article  PubMed  Google Scholar 

  • A Liekfeld F Schweig C Jaeckel K-D Wernecke C Hartmann U Pleyer (2000) ArticleTitleIntraocular antibody production in intraocular inflammation Graefe’s Arch Clin Exp Ophthalmol 238 222–227 Occurrence Handle1:STN:280:DC%2BD3c3lsFSmsQ%3D%3D Occurrence Handle10.1007/s004170050347

    Article  CAS  Google Scholar 

  • MI Lynch F Cordeiro S Ferreira R Ximenes F Oréfice E Malagueño (2004) ArticleTitleLacrimal secretory JgA in active posterior uveitis induced by Toxoplasma gondii Mem Inst Oswaldo Cruz, Rio de Janeiro 99 861–864 Occurrence Handle1:CAS:528:DC%2BD2MXhsFCksbY%3D

    CAS  Google Scholar 

  • HN Madhavan J Biswan L Gopal T Sharma (1995) ArticleTitleInvestigations in the virological etiology in acute retinal inflammation Indian J Med Res 101 233–237 Occurrence Handle7672832 Occurrence Handle1:STN:280:DyaK2Mvgt12qsA%3D%3D

    PubMed  CAS  Google Scholar 

  • HN Madhavan K Priya (2003) ArticleTitleThe diagnostic significance of enzyme linked immuno-sorbent assay for Herpes simplex, Varicella zoster and Cytomegalovirus retinitis Indian J Ophthalmol 51 71–75 Occurrence Handle12701866

    PubMed  Google Scholar 

  • NN Markomichelakis C Canakis P Zafirakis T Marakis I Millias G Theodossiadis (2002) ArticleTitleCytomegalovirus as a cause of anterior uveitis with sectoral iris atrophy Ophthalmology 109 879–882 Occurrence Handle11986091 Occurrence Handle10.1016/S0161-6420(02)00961-2

    Article  PubMed  Google Scholar 

  • BW McBride KA Ward (1987) ArticleTitleHerpes simplex-specific IgG subclass response in herpetic keratitis J Med Virol 21 179–189 Occurrence Handle3029321 Occurrence Handle1:STN:280:DyaL2s7ktVSjtA%3D%3D

    PubMed  CAS  Google Scholar 

  • JD McCann TP Margolis MG Wong BD Kuppermann AP Luckie DM Schwartz AR Irvine E Al (1995) ArticleTitleA sensitive and specific polymerase chain reaction-based assay for the diagnosis of Cytomegalovirus retinitis Am J Ophthalmol 120 219–226 Occurrence Handle7639306 Occurrence Handle1:CAS:528:DyaK2MXnsFejsrs%3D

    PubMed  CAS  Google Scholar 

  • SM Mitchell JD Fox (1995) ArticleTitleAqueous and vitreous humor samples for the diagnosis of Cytomegalovirus retinitis Am J Ophthalmol 120 252–256 Occurrence Handle7639312 Occurrence Handle1:STN:280:DyaK2Mzms1Gnsw%3D%3D

    PubMed  CAS  Google Scholar 

  • RB Nussenblatt (1996) Acquired immunodeficiency syndrome RB Nussenblatt SM Whitcup AG Palestine (Eds) Uveitis EditionNumber2 SeriesTitleFundamentals and clinical practice Mosby London 186–197

    Google Scholar 

  • RB Nussenblatt (1996) Examination of the Patient with uveitis RB Nussenblatt SM Whitcup AG Palestine (Eds) Uveitis EditionNumber2 SeriesTitleFundamentals and clinical practice Mosby London 58–68

    Google Scholar 

  • K Pathanapitoon S Ausayakhum P Kunavisarut A Pungrasame W Sirirungsi (2005) ArticleTitleDetection of cytomegalovirus in vitreous, aqueous and conjunctiva by polymerase chain reaction (PCR) J Med Assoc Thai 88 228–232 Occurrence Handle15962675

    PubMed  Google Scholar 

  • B Pedersen S Moller Andersen A Klauber E Ottovay JU Prause CU Zhong B Norrild (1982) ArticleTitleSecretory IgA specific for Herpes simplex virus in lacrimal fluid from patients with herpes keratitis – a possible diagnostic parameter Br J Ophthalmol 66 648–653 Occurrence Handle6288066 Occurrence Handle1:STN:280:DyaL3s%2FgtVaisw%3D%3D

    PubMed  CAS  Google Scholar 

  • NP Pramond E Dhevahi K Sudhamathi K Kannan SP Thyagarajan (1999) ArticleTitleTear secretory IgA: evaluation of usefulness as a diagnostic marker in herpetic keratitis Ocul Immunol Inflamm 7 61–67 Occurrence Handle10.1076/ocii.7.2.61.4023

    Article  Google Scholar 

  • P-Y Robert I Traccard J-P Adenis F Denis S Ranger-Rogez (2002) ArticleTitleMultiplex detection of Herpesviruses in tear fluid using the “Stair Primers” PCR method: prospective study of 93 patients J Med Virol 66 506–511 Occurrence Handle11857529 Occurrence Handle1:CAS:528:DC%2BD38Xit1aqsLk%3D Occurrence Handle10.1002/jmv.2173

    Article  PubMed  CAS  Google Scholar 

  • EB Rozanova (1982) ArticleTitleCytomegalovirus: their role in human pathology Review Vestn Oftalmol 1 68–71

    Google Scholar 

  • EB Rozanova (1984) Immunological diagnosis of Cytomegalovirus- and herpetic infections in patients with pathology of retina and uveal tract Medicina Russia

    Google Scholar 

  • RC Siqueira A Cunha F Oréfice WR Campos LTM Figueiredo (2004) ArticleTitlePCR with aqueous humor, blood leukocytes and vitreous of patients affected by cytomegalovirus retinitis and immune recovery uveitis Ophthalmologica 218 43–48 Occurrence Handle14688435 Occurrence Handle1:CAS:528:DC%2BD3sXhtVSjt7nP Occurrence Handle10.1159/000074566

    Article  PubMed  CAS  Google Scholar 

  • RN Van Gelder JL Willig GN Holland HJ Kaplan (2001) ArticleTitleHerpes simplex virus type 2 as a cause of acute retinal necrosis syndrome in young patients Ophthalmology 1008 869–876 Occurrence Handle10.1016/S0161-6420(01)00556-5

    Article  Google Scholar 

  • A Voller DE Bidwell A Bartlett (1976) ArticleTitleEnzyme immunoassays in diagnostic medicine. Theory and practice Bull World Health Organ 53 55–65 Occurrence Handle1085667 Occurrence Handle1:CAS:528:DyaE28XltFyqtrY%3D

    PubMed  CAS  Google Scholar 

  • A Voller DE Bidwell (1976) ArticleTitleEnzyme immunoassay for antibodies in measles, cytomegalovirus infection and after rubella vaccination Br J Exp Pathol 57 243–247 Occurrence Handle178338 Occurrence Handle1:STN:280:DyaE287osVClsg%3D%3D

    PubMed  CAS  Google Scholar 

  • JB Wand SP Adler (1996) ArticleTitleSalivary antibodies to cytomegalovirus (CMV) glycoprotein B accurately predict CMV infections among preschool children J Clin Microbiol 34 2632–2634

    Google Scholar 

  • SME Wren AR Fielder D Bethell EGH Lyall G Tudor-Williams KD Cocker SM Mitchell (2004) ArticleTitleCytomegalovirus retinitis in infancy Eye 18 389–392 Occurrence Handle15069436 Occurrence Handle1:STN:280:DC%2BD2c7nsVOhuw%3D%3D Occurrence Handle10.1038/sj.eye.6700696

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Immunology and Virology, Helmholtz Research Institute of Eye Diseases, Sadovaya-Chernogriazskaya, Moscow, Russia

    E. B. Rozanova & L. E. Teplinskaia

  2. Department of Retina and Optic Nerve, Helmholtz Research Institute of Eye Diseases, Sadovaya-Chernogriazskaya, Moscow, Russia

    A. F. Kaliberdina

  3. Department of Ophthalmology, Medical University of Vienna, Vienna, Austria

    E. B. Rozanova & T. Barisani-Asenbauer

Authors
  1. E. B. Rozanova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. E. Teplinskaia
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. A. F. Kaliberdina
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Barisani-Asenbauer
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rozanova, E., Teplinskaia, L., Kaliberdina, A. et al. Cytomegalovirus antibodies in tear fluid of patients with retinitis. Arch Virol 151, 2407–2417 (2006). https://doi.org/10.1007/s00705-006-0813-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00705-006-0813-0

Keywords

Search

Navigation