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Detection and identification of avian, duck, and goose reoviruses by RT-PCR: goose and duck reoviruses are part of the same genogroup in the genus Orthoreovirus

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Summary.

A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck, and goose reovirus (ARV, DRV, and GRV) RNA from cell culture supernatant and clinical samples was established. Based on multiple sequence alignment, a pair of degenerate primers was selected and synthesized. The amplified, cloned, and sequenced 598-base-pair products from the σA-encoding gene fragment from 16 isolates (ranging over 30 years) indicated that the primer regions were well conserved. The sensitivity of this method was determined to be 10−2 PFU. The specificity of the RT-PCR method was determined by testing specimens containing avian influenza A viruses, Newcastle disease virus, and infectious bronchitis virus, all of which yielded negative results with no discernible background. The efficiency of the system for detection of ARV, DRV, and GRV directly in 71/83 clinical samples was confirmed. The nucleotide sequence analysis indicated that DRV and GRV isolated from China in different locales and years were closely related, showing 97.4–100% homology to each other, but with only 86.7–88.5% identity to DRV 89026. The nucleotide and amino acid sequence identities in the amplified σA-encoding gene were 74.2–78.4% and 86.9–92.0%, respectively, between duck/goose and chicken species. Phylogenetic analysis indicated that GRV and DRV aggregated into the same specified genogroup within subgroup II of the genus Orthoreovirus and are more closely related to ARV than to Nelson Bay virus. Overall, this study developed a sensitive and specific technique for the identification ARV, DRV, and GRV, and sequencing analysis has enhanced our understanding of the evolutionary relationship between ARV, DRV, and GRV.

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References

  • BM Adair K Burns ER Mckillop (1987) ArticleTitleSerological studies with reoviruses in chickens, turkeys and ducks J Comp Pathol 97 495–501 Occurrence Handle3119679 Occurrence Handle1:STN:280:BieD2MbosFI%3D Occurrence Handle10.1016/0021-9975(87)90001-6

    Article  PubMed  CAS  Google Scholar 

  • S Bruhn L Bruckner HP Ottiger (2005) ArticleTitleApplication of RT-PCR for the detection of avian reovirus contamination in avian viral vaccines J Virol Methods 123 179–186 Occurrence Handle15620400 Occurrence Handle1:CAS:528:DC%2BD2cXhtFGht73N Occurrence Handle10.1016/j.jviromet.2004.09.019

    Article  PubMed  CAS  Google Scholar 

  • TS Dermody ML Nibert R Bassel-Duby BN Fields (1990) ArticleTitleSequence diversity in S1 genes and S1 translation products of eleven type 3 reovirus J Virol 64 4842–4850 Occurrence Handle2398530 Occurrence Handle1:CAS:528:DyaK3MXhvFKgsg%3D%3D

    PubMed  CAS  Google Scholar 

  • DE Docherty PG Slota (1988) ArticleTitleUse of muscovy duck embryo fibroblasts for the isolation of viruses from wild birds J Tissue Cult Methods 11 165–170 Occurrence Handle10.1007/BF01404270

    Article  Google Scholar 

  • R Duncan (1999) ArticleTitleExtensive sequence divergence and phylogenetic relationships between the fusogenic and nofusogenic orthoreoviruses: a species proposal Virology 260 316–328 Occurrence Handle10417266 Occurrence Handle1:CAS:528:DyaK1MXksFChsbg%3D Occurrence Handle10.1006/viro.1999.9832

    Article  PubMed  CAS  Google Scholar 

  • DD Erdman GA Weinberg EKM Weinberg FJ Walker BC Anderson J Winter M González LJ Anderson (2003) ArticleTitleGenescan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness J Clin Microbiol 41 4298–4303 Occurrence Handle12958260 Occurrence Handle1:CAS:528:DC%2BD3sXnvFaqtbo%3D Occurrence Handle10.1128/JCM.41.9.4298-4303.2003

    Article  PubMed  CAS  Google Scholar 

  • G Eugene-Ruellan F Freymuth C Bahloul H Badrane A Vabret N Tordo (1998) ArticleTitleDetection of respiratory syncytial virus A and B and parainfluenza virus 3 sequences in respiratory tracts of infants by single PCR with primers targeted to the L-polymerase gene and differential hybridization J Clin Microbiol 36 796–801 Occurrence Handle9508315 Occurrence Handle1:CAS:528:DyaK1cXhsVGiuro%3D

    PubMed  CAS  Google Scholar 

  • D Gaudry J Charles J Tektoff (1972) ArticleTitleA new disease expressing itself by a viral pericarditis in Barbary ducks C R Acad Sci 274 2916–2929 Occurrence Handle1:STN:280:CS2B38fgsV0%3D

    CAS  Google Scholar 

  • SE Glass AS Naqi CF Hall KM Kerr (1973) ArticleTitleIsolation and characterization of a virus associated with arthritis of chickens Avian Dis 17 415–424 Occurrence Handle4351420 Occurrence Handle1:STN:280:CSyB3c3ovVU%3D Occurrence Handle10.2307/1589226

    Article  PubMed  CAS  Google Scholar 

  • U Heffels-Redmann H Müller EF Kaleta (1992) ArticleTitleStructural and biological characteristics of reoviruses isolated from muscovy ducks (Cairina moschata) Avian Pathol 21 481–491

    Google Scholar 

  • T Hollmém JC Franson M Kilpi DE Docherty WR Hansen M Hario (2002) ArticleTitleIsolation and characterization of a reovirus from common eiders (Somateria mollissima) from Finland Avian Dis 46 478–484 Occurrence Handle10.1637/0005-2086(2002)046[0478:IACOAR]2.0.CO;2

    Article  Google Scholar 

  • RC Jones JRM Guneratne (1984) ArticleTitleThe pathogenicity of some reoviruses with particular reference to tenosynovitis Avian Pathol 13 173–189

    Google Scholar 

  • VR Kaschula (1950) ArticleTitleA new virus disease of the muscovy duck (Cairina moschata Linn.) present in Natal J S Afr Vet Med Assoc 21 18–26

    Google Scholar 

  • G Kuntz-Simon G Le-Reculé C De Boisséson V Jestin (2002) ArticleTitleMuscovy duck reovirus σC protein is atypically encoded by the smallest genome segment J Gen Virol 83 1189–1200 Occurrence Handle11961275 Occurrence Handle1:CAS:528:DC%2BD38XjsFWmsbs%3D

    PubMed  CAS  Google Scholar 

  • LH Lee JH Shien HK Shieh (1998) ArticleTitleDetection of avian reovirus RNA and comparison of a portion of genome segment S3 by polymerase chain reaction and restriction enzyme fragment length polymorphism Res Vet Sci 65 11–15 Occurrence Handle9769066 Occurrence Handle1:STN:280:DyaK1cvktVSjsA%3D%3D Occurrence Handle10.1016/S0034-5288(98)90020-0

    Article  PubMed  CAS  Google Scholar 

  • G Le Gall-Reculé M Cherbonnel C Arnauld P Blanchard A Jestin V Jestin (1999) ArticleTitleMolecular characterization and expression of the S3 gene of muscovy duck reovirus strain 89026 J Gen Virol 80 195–203 Occurrence Handle9934702

    PubMed  Google Scholar 

  • O Legeay Y Caudrelier C Cordevant L Rigottier-Gois M Lange (2000) ArticleTitleSimplified procedure for detection of enteric pathogenic viruses in shellfish by RT-PCR J Virol Methods 90 IssueID1 1–14 Occurrence Handle11011076 Occurrence Handle1:CAS:528:DC%2BD3cXmslyjtbk%3D Occurrence Handle10.1016/S0166-0934(00)00174-9

    Article  PubMed  CAS  Google Scholar 

  • HJ Liu MH Liao CD Chang JH Chen MY Lin MC Tung (1999) ArticleTitleComparison of two molecular techniques for the detection of avian reoviruses in formalin-fixed, paraffin-embedded chicken tissues J Virol Methods 80 197–201 Occurrence Handle10471029 Occurrence Handle1:CAS:528:DyaK1MXmtVSrsbY%3D Occurrence Handle10.1016/S0166-0934(99)00047-6

    Article  PubMed  CAS  Google Scholar 

  • HJ Liu PH Huang (2001) ArticleTitleSequence and phylogenetic analysis of the sigmaA-encoding gene of avian reovirus J Virol Methods 98 99–107 Occurrence Handle11576636 Occurrence Handle1:CAS:528:DC%2BD3MXntV2ju74%3D Occurrence Handle10.1016/S0166-0934(01)00328-7

    Article  PubMed  CAS  Google Scholar 

  • HJ Liu HH Chen MH Liao MY Lin GN Chang (1999) ArticleTitleIdentification of the sigma C-encoded gene of avian reovirus by nested PCR and restriction endonuclease analysis J Virol Methods 81 83–90 Occurrence Handle10488765 Occurrence Handle1:CAS:528:DyaK1MXksFajurk%3D Occurrence Handle10.1016/S0166-0934(99)00063-4

    Article  PubMed  CAS  Google Scholar 

  • M Malkinson K Perk Y Weisman (1981) ArticleTitleReovirus infection of young muscovy ducks (Cairina moschata) Avian Pathol 10 433–440

    Google Scholar 

  • V Marius-Jestin M Lagadic Y Le Menec G Bennejean (1988) ArticleTitleHistological data associated with muscovy duck reovirus infection Vet Rec 123 32–33 Occurrence Handle2845626 Occurrence Handle1:STN:280:BiaD38%2FmsVU%3D

    PubMed  CAS  Google Scholar 

  • J Maritinez-Costas C Gonzalez-Lepez VN Vakharia J Benavente (2000) ArticleTitlePossible involvement of the double-stranded RNA-binding core protein σA in the resistance of avian reovirus to interferon J Virol 74 1124–1131 Occurrence Handle10.1128/JVI.74.3.1124-1131.2000

    Article  Google Scholar 

  • JB McFerran TJ Connor RM McCracken (1976) ArticleTitleIsolation adenoviruses and reoviruses from avian species other than domestic fowl Avian Dis 20 519–524 Occurrence Handle962759 Occurrence Handle1:STN:280:CSmB2sjks1c%3D Occurrence Handle10.2307/1589384

    Article  PubMed  CAS  Google Scholar 

  • NA Menendez BW Calnek BS Cowen (1975) ArticleTitleLocalization of avian reoviruses (FDO isolate) in tissues of mature chickens Avian Dis 19 112–117 Occurrence Handle164175 Occurrence Handle1:STN:280:CSqC38jitFQ%3D Occurrence Handle10.2307/1588961

    Article  PubMed  CAS  Google Scholar 

  • V Palya R Glávits M Dobos-Kovács E Ivanics E Nagy K Bányai G Reuter G Szücs A Dán M Benkö (2003) ArticleTitleReovirus identified as cause of disease in young geese Avian Pathol 32 129–138 Occurrence Handle12745366 Occurrence Handle10.1080/0307945021000071879

    Article  PubMed  Google Scholar 

  • S Pascucci D Gelmetti L Giovanetti (1984) ArticleTitleInfezione da reovirus dell’anitra muschiata (Cairina moschata) Clin Vet 107 148–151

    Google Scholar 

  • JK Rosenberger NO Olson (1997) Viral arthritis BW Calnek (Eds) Diseases of poultry EditionNumber10 Iowa State University Press Ames 711–719

    Google Scholar 

  • TJ Schnitzer T Ramos V Gouvea (1982) ArticleTitleAvian reovirus polypeptides: analysis of intracellular virus-specified products virions, top component, and cores J Virol 43 1006–1014 Occurrence Handle7143561 Occurrence Handle1:CAS:528:DyaL38XlsVWgu7s%3D

    PubMed  CAS  Google Scholar 

  • ML Spinner GD Giovanni (2001) ArticleTitleDetection and identification of mammalian reoviruses in surface water by combined cell culture and reverse transcription-PCR Appl Environ Microbiol 67 3016–3020 Occurrence Handle11425715 Occurrence Handle1:CAS:528:DC%2BD3MXkvFSntb0%3D Occurrence Handle10.1128/AEM.67.7.3016-3020.2001

    Article  PubMed  CAS  Google Scholar 

  • SG Thayer CW Beard (1998) Serologic procedures DE Swayne JR Glisson MW Jackwood JE Pearson WM Reed (Eds) A laboratory manual for the isolation and identification of avian pathogens EditionNumber4 Am Assoc Avian Pathologists Kennett Square, PA 255–266

    Google Scholar 

  • PL Thomas JC Erker ML Chalmers AT Cruz JD Wetzel SM Desai IK Mushahwar TS Dermody (2002) ArticleTitleDetection of mammalian reovirus RNA by using reverse transcription-PCR: sequence diversity within the λ3-encoding l1 gene J Clin Microbiol 40 1368–1375 Occurrence Handle10.1128/JCM.40.4.1368-1375.2002

    Article  Google Scholar 

  • MHV Van Regenmortel CM Fauquet DHL Bishop EB Carstens MK Estes SM Lemon J Maniloff MA Mayo DJ McGeoch CR Pringle RB Wickner (2000) Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses Academic Press San Diego 399–480

    Google Scholar 

  • AP Wyn-Jones R Pallin C Dedoussis J Shore J Sellwood (2000) ArticleTitleThe detection of small round-structured viruses in water and environmental materials J Virol Methods 87 99–107 Occurrence Handle10856757 Occurrence Handle1:CAS:528:DC%2BD3cXktVSnsL0%3D Occurrence Handle10.1016/S0166-0934(00)00157-9

    Article  PubMed  CAS  Google Scholar 

  • HS Yin HK Shieh LH Lee (2000) ArticleTitleSynthesis in Escherichia coli of avian reovirus core protein σA and its dsRNA-binding activity Virology 266 33–41 Occurrence Handle10612658 Occurrence Handle1:CAS:528:DyaK1MXotFGksLY%3D Occurrence Handle10.1006/viro.1999.0020

    Article  PubMed  CAS  Google Scholar 

  • Zhang Y, Liu M, Hu QL, Ouyang SD (2006) Characterization of σC-encoding gene from muscovy duck reovirus. Virus Gene 32(2): in press

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Authors and Affiliations

  1. National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, P.R. China

    Y. Zhang, M. Liu, O. Shuidong, D. C. Guo, H. Y. Chen & Z. Han

  2. Virology Divisions, Fujian Husbandry and Veterinary Research Institute, Fujian, P.R. China

    O. Shuidong & Q. L. Hu

  3. Veterinary Department, Northeast Agriculture University, Harbin, P.R. China

    Z. Han

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  1. Y. Zhang
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  2. M. Liu
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Zhang, Y., Liu, M., Shuidong, O. et al. Detection and identification of avian, duck, and goose reoviruses by RT-PCR: goose and duck reoviruses are part of the same genogroup in the genus Orthoreovirus . Arch Virol 151, 1525–1538 (2006). https://doi.org/10.1007/s00705-006-0731-1

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  • DOI: https://doi.org/10.1007/s00705-006-0731-1

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