Summary.
Comparison of nucleotide sequences at the VP1 coding region of foot-and-mouth disease serotype Asia1 viruses from India has revealed two genetic lineages with emergence of a genetically divergent group in recent years. In this study a simple, fast, relatively costeffective multi-primer RT-PCR assay to differentiate genetic lineages of type Asia1 viruses was developed. Efforts were made in the design of novel lineage-specific primers and in optimization of the multi-primer assay protocol in conjunction with the use of the serotype specific primer for confirmation of serotype Asia1 virus. This assay promises to be an effective tool in molecular epidemiological investigation of FMD in the country.
References
S Alexandersen MA Forsyth SM Reid GJ Belsham (2000) ArticleTitleDevelopment of reverse transcription-PCR (oligonucleotide probing) enzyme linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: a new system using simple and aqueous phase hybridization. J Clin Microbiol 38 4604–4613 Occurrence Handle1:CAS:528:DC%2BD3MXislCksr0%3D Occurrence Handle11101603
E Domingo C Escarmis MA Martinez E Martinez-Salas MG Mateu (1992) ArticleTitleFoot-and mouth disease virus populations are quasispecies. Curr Top Microbiol Immunol 176 33–47 Occurrence Handle1:CAS:528:DyaK38Xmt12lt7c%3D Occurrence Handle1318185
P Giridharan D Hemadri C Tosh A Sanyal SK Bandyopadhyay (2005) ArticleTitleDevelopment and evaluation of a multiplex PCR for differentiation of foot-and-mouth disease strains native to India. J Virol Methods 126 1–11 Occurrence Handle10.1016/j.jviromet.2005.01.015 Occurrence Handle1:CAS:528:DC%2BD2MXjsVGmu7s%3D Occurrence Handle15847913
CB Gurumurthy A Sanyal R Venkataramanan C Tosh M George D Hemadri (2002) ArticleTitleGenetic diversity in the VP1 gene of foot-and-mouth disease virus serotype Asia 1. Arch Virol 147 85–102 Occurrence Handle10.1007/s705-002-8304-y Occurrence Handle1:CAS:528:DC%2BD38XitFSrs7o%3D Occurrence Handle11855637
Knowles NJ, Samuel AR (1995) Polymerase chain reaction amplification and cycle sequencing of 1D (VP1) gene of foot-and-mouth disease viruses, pp 45–53. In: Rpt Session of the Research Group of the Standing Technical Committee of the European Commission for the Control of Foot-and-Mouth Disease, Vienna, Austria, 19–22 September 1994
Kumar S, Tamura K, Jakobsen IB, Nei M (2001) MEGA2: Molecular Evolutionary Genetics Analysis software. Arizona State University, Tempe, Arizona, USA
JK Mohapatra A Sanyal D Hemadri C Tosh GP Sabarinath R Manoj Kumar R Venkataramanan SK Bandyopadhyay (2004) ArticleTitleSequence variability in the structural protein-encoding region of foot-and-mouth disease virus serotype Asia1field isolates. Res Vet Sci 77 153–161 Occurrence Handle10.1016/j.rvsc.2004.03.003 Occurrence Handle1:CAS:528:DC%2BD2cXkvVensrs%3D Occurrence Handle15196905
JK Mohapatra A Sanyal D Hemadri C Tosh GP Sabarinath R Venkataramanan (2002) ArticleTitleSequence and phylogenetic analysis of the L and VP1 genes of foot-and-mouth disease virus serotype Asia 1. Virus Res 87 107–118 Occurrence Handle10.1016/S0168-1702(02)00006-0 Occurrence Handle1:CAS:528:DC%2BD38XmtlCksL4%3D Occurrence Handle12191774
A Sanyal D Hemadri C Tosh SK Bandyopadhyay (2004) ArticleTitleEmergence of a novel subgroup within the widely circulating lineage of Foot-and-Mouth Disease virus serotype Asia1 in India. Res Vet Sci 76 151–156 Occurrence Handle10.1016/j.rvsc.2003.09.002 Occurrence Handle1:CAS:528:DC%2BD3sXps1equ74%3D Occurrence Handle14672859
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Mohapatra, J., Sanyal, A., Hemadri, D. et al. A novel genetic lineage differentiating RT-PCR as a useful tool in molecular epidemiology of foot-and-mouth disease in India. Arch Virol 151, 803–809 (2006). https://doi.org/10.1007/s00705-005-0673-z
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DOI: https://doi.org/10.1007/s00705-005-0673-z