Summary.
A novel virus was identified in banana (Musa spp). Analysis of the last 2917 nucleotides of its positive strand genomic RNA showed five open reading frames corresponding, from 5′ to 3′, to a truncated ORF coding for a replication-associated protein, three ORFs coding for a movement-associated triple gene block (TGB) and a capsid protein (CP) gene. This genome organization is similar to that of some members of the Flexiviridae family such as potexviruses and foveaviruses. This virus was named Banana virus X (BVX). Comparative sequence analysis showed that BVX is only distantly related to other members of the Flexiviridae family, in which it appears to define a new genus. BVX produces defective RNAs derived from its genomic RNA by non-homologous recombination. Three distinct pairs of donor/acceptor recombination sites involving short direct nucleotide repeats were characterized, accounting for deletions of 1268, 1358 and 1503 nucleotides. Contrary to the situation encountered for Potexviruses, these recombination sites are located within the TGB1 and CP genes and result in a truncated TGB1 protein.
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MJ Adams JF Antoniw M Bar-Joseph AA Brunt T Candresse GD Foster GP Martelli RG Milne CM Fauquet (2004) ArticleTitleThe new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation. Arch Virol 149 1045–1060 Occurrence Handle15098118
LA Calvert MI Cuervo MD Ospina CM Fauquet BC Ramirez (1996) ArticleTitleCharacterization of Cassava common mosaic virus and a defective RNA species. J Gen Virol 77 525–530 Occurrence Handle8601791
S Chang J Puryear J Cairney (1993) ArticleTitleA simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11 113–116
S Dallot P Acuna C Rivera P Ramirez F Cote BE Lockhart ML Caruana (2001) ArticleTitleEvidence that the proliferation stage of micropropagation procedure is determinant in the expression of banana streak virus integrated into the genome of the FHIA 21 hybrid (Musa AAAB). Arch Virol 146 2179–2190 Occurrence Handle10.1007/s007050170028 Occurrence Handle11765919
CF Gambley JE Thomas (2001) ArticleTitleMolecular characterisation of banana mild mosaic virus, a new filamentous virus in Musa spp. Arch Virol 146 1369–1379 Occurrence Handle10.1007/s007050170097 Occurrence Handle11556712
Hull R (2002) Plant virology. Academic Press, San Diego
Jones DR (2000) Diseases of banana, abacá and enset. CABI Publishing, Wallingford, UK, pp 241–293
TBL Kirkwood CRM Bangham (1994) ArticleTitleCycles, chaos and evolution in virus cultures: a model of defective interfering particles. Proc Natl Acad Sci USA 91 8685–8689 Occurrence Handle8078942
EV Koonin VV Dolja (1993) ArticleTitleEvolution and taxonomy of positive-strand RNA viruses: implication of comparative analysis of amino acid sequences. Crit Rev Biochem Mol Biol 28 375–430 Occurrence Handle8269709
S Kumar K Tamura IB Jakobsen M Nei (2001) ArticleTitleMEGA2: Molecular Evolutionary Genetics Analysis (MEGA) software. Bioinformatics 17 1244–1245 Occurrence Handle10.1093/bioinformatics/17.12.1244 Occurrence Handle11751241
Y Liu DJ Robinson BD Harrison (1998) ArticleTitleDefective forms of cotton leaf curl virus DNA-A that have different combinations of sequence deletion, duplication inversion and rearrangement. J Gen Virol 79 1501–1508 Occurrence Handle9634094
M Mawassi E Mietiewska ME Hilf L Ashoulin AV Karasev (1995) ArticleTitleMultiple species of defective RNAs in plants infected with citrus tristeza virus. Virology 214 264–268 Occurrence Handle10.1006/viro.1995.9930 Occurrence Handle8525626
TJ Morris JA Dodds (1979) ArticleTitleIsolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69 854–858
DR Page (1996) ArticleTitleTreeView: an application to display phylogenetic trees on personal computer. Comput Appl Biosci 12 357–358 Occurrence Handle8902363
L Roux AE Simon JJ Holland (1991) ArticleTitleEffects of defective interfering viruses on virus replication and pathogenesis in vitro and in vivo. Adv Virus Res 40 181–211 Occurrence Handle1957718
S Sawyer (1989) ArticleTitleStatistical tests for detecting gene conversion. Mol Biol Evol 6 526–538 Occurrence Handle2677599
N Shapka PD Nagy (2004) ArticleTitleThe AU-rich RNA recombination hotspot sequence of brome mosaic virus is functional in Tombusviruses: implications for the mechanism of RNA recombination. J Virol 78 2288–2300 Occurrence Handle10.1128/JVI.78.5.2288-2300.2004 Occurrence Handle14963125
DC Stenger (1995) ArticleTitleGenotypic variability and the occurrence of less then genome-length viral DNA forms in a field popumation of beet curly top geminivirus. Phytopathology 85 1316–1322
JD Thompson DG Higgins TJ Gibson (1994) ArticleTitleCLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 11 4673–4680
JD Thompson TJ Gibson F Plewniak F Jeanmougin DG Higgins (1997) ArticleTitleThe Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Res 24 4876–4882 Occurrence Handle10.1093/nar/25.24.4876
RA Valverde JA Dodds JA Heick (1986) ArticleTitleDouble-stranded ribonucleic acid from plants infected with viruses having elongated particles and undivided genomes. Phytopathology 76 459–465
KA White JB Bancroft GA Mackie (1991) ArticleTitleDefective RNAs of clover yellow mosaic virus encode nonstructural/coat protein fusion products. Virology 183 479–486 Occurrence Handle10.1016/0042-6822(91)90977-J Occurrence Handle1830181
KA White JB Bancroft GA Mackie (1992) ArticleTitleCoding capacity determines in vivo accumulation of a defective RNA of clover yellow mosaic virus. Proc Natl Acad Sci USA 66 3069–3076
KA White TJ Morris (1999) ArticleTitleDefective and defective interfering RNAs of monopartite plus-strand RNA plant viruses. Curr Top Microbiol Immunol 239 1–17 Occurrence Handle9893366
TY Yeh YC Lin YC Chang YH Hsu NS Lin (1999) ArticleTitleA defective RNA associated with bamboo mosaic virus and the possible common mechanisms for RNA recombination in potexviruses. Virus Genes 18 121–128 Occurrence Handle10.1023/A:1008008400653 Occurrence Handle10403698
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Teycheney, PY., Marais, A., Svanella-Dumas, L. et al. Molecular characterization of banana virus X (BVX), a novel member of the Flexiviridae family. Arch Virol 150, 1715–1727 (2005). https://doi.org/10.1007/s00705-005-0567-0
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DOI: https://doi.org/10.1007/s00705-005-0567-0