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A brief review on microfluidic platforms for hormones detection

  • Translational Neurosciences - Review Article
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Abstract

Lab-on-chip technology is attracting great interest due to its potential as miniaturized devices that can automate and integrate many sample-handling steps, minimize consumption of reagent and samples, have short processing time and enable multiplexed analysis. Microfluidic devices have demonstrated their potential for a broad range of applications in life sciences, including point-of-care diagnostics and personalized medicine, based on the routine diagnosis of levels of hormones, cancer markers, and various metabolic products in blood, serum, etc. Microfluidics offers an adaptable platform that can facilitate cell culture as well as monitor their activity and control the cellular environment. Signaling molecules released from cells such as neurotransmitters and hormones are important in assessing the health of cells and the effect of drugs on their functions. In this review, we provide an insight into the state-of-art applications of microfluidics for monitoring of hormones released by cells. In our works, we have demonstrated efficient detection methods for bovine growth hormones using nano and microphotonics integrated microfluidics devices. The bovine growth hormone can be used as a growth promoter in dairy farming to enhance the milk and meat production. In the recent years, a few attempts have been reported on developing very sensitive, fast and low-cost methods of detection of bovine growth hormone using micro devices. This paper reviews the current state-of-art of detection and analysis of hormone using integrated optical micro and nanofluidics systems. In addition, the paper also focuses on various lab-on-a-chip technologies reported recently, and their benefits for screening growth hormones in milk.

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Abbreviations

AD:

Androstene-dione

CWC:

Cascaded waveguide coupler

E2:

Estradiol

ELISA:

Enzyme-linked immunosorbent assay 

−HV:

Negative high voltage

LIF:

Laser induced fluorescence

LOC:

Lab-on-a-chip

LSPR:

Localized surface plasmon resonance

μELISA:

Enzyme-linked immunosorbent assay

μTAS:

Micro total analysis systems

PDMS:

Polydimethylsiloxane

PG:

Progesterone

POC:

Point-of-care

PON:

Point-of-need

rbST:

Recombinant bovine somatotropin

RXN:

Reaction

TS:

Testosterone

TSH:

Thyroid-stimulating hormone

HPLC:

High performance liquid chromatography

MS:

Mass spectroscopy

FITC:

Fluorescein isothiocyanate

SOS:

Silica-on-silicon

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Acknowledgments

The authors thank NSERC and Concordia Research Chair for the financial support.

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Authors and Affiliations

  1. Optical-Bio Microsystems Laboratory, Department of Mechanical and Industrial Engineering, Concordia University, Montreal, QC, H3G 1M8, Canada

    Jayan Ozhikandathil, Simona Badilescu & Muthukumaran Packirisamy

Authors
  1. Jayan Ozhikandathil
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  2. Simona Badilescu
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  3. Muthukumaran Packirisamy
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Correspondence to Muthukumaran Packirisamy.

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Ozhikandathil, J., Badilescu, S. & Packirisamy, M. A brief review on microfluidic platforms for hormones detection. J Neural Transm 124, 47–55 (2017). https://doi.org/10.1007/s00702-016-1610-x

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  • DOI: https://doi.org/10.1007/s00702-016-1610-x

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