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Expression of extracellular matrix-proteins in perisellar connective tissue and dura mater

  • Experimental Research
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Abstract

Purpose

To describe the pattern of expression of extracellular matrix (ECM) proteins in perisellar connective tissue.

Methods

Dural and perisellar specimens from ten individuals were investigated immunohistochemically for collagens I to IV, tenascin, fibronectin, elastin, laminin, and vitronectin.

Findings

Collagen I and III and fibronectin were strongly expressed and collagen IV, tenascin, and vitronectin were moderately expressed in the boundaries of the sella and around the CS. In six of nine specimens from the anterior boundary of the sella, and in 11 of 19 samples from the lateral boundary of the sella (medial wall of CS), two different layers could be detected by the expression of different ECM proteins. None of the antigens generally allowed differentiation between two layers of the pituitary envelope.

Conclusions

The pituitary boundary may consist of a single or a double layer, infrequently differentiated from each other by the expression of different ECM proteins.

Summary

Background

The medial wall (MW) of the cavernous sinus (CS) was described as fragile with small defects allowing pituitary adenomas to penetrate it, whereas other publications focus on biological properties of pituitary adenomas leading to cavernous sinus infiltration. Against this background, we describe the pattern of expression of extracellular matrix (ECM) proteins in perisellar connective tissue as possible targets for tumor-derived proteinases.

Methods

Post-mortem specimens were obtained from ten individuals without endocrinological disorders: diaphragma sellae, anterior and lateral wall of pituitary boundary (MW of CS), lateral wall of CS, convexity dura, tentorial dura, temporal fascia, intracavernous internal carotid artery (ICA), and internal jugular vein. Immunohistochemical detection of collagens I to IV, tenascin, fibronectin, elastin, laminin, and vitronectin was performed.

Findings

Collagens I and III were strongly expressed and collagen II weakly expressed, and collagen IV moderately expressed in the lateral as well as anterior boundaries of the sella and around the CS. Fibronectin was strongly expressed, tenascin and vitronectin were moderately expressed in the edge of the sellar compartment and dura mater, whereas expression of elastin and laminin was weak or absent. In six of nine specimens derived from the anterior boundary of the sella, and in 11 of 19 samples obtained from the lateral boundary of the sella (MW of CS), two different layers could be detected by the expression of different ECM proteins. None of the antigens investigated generally allowed differentiation between two layers of the pituitary covering.

Conclusions

The pituitary boundary may consist of a single or a double layer. Infrequently the pituitary capsule can be differentiated from dura mater concerning the expression of different ECM proteins. The combination of individual anatomy and pattern of ECM expression in a given case, and biological properties of the adenoma may be important for the occurrence of CS infiltration.

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Acknowledgements

The authors appreciated the excellent help of Mrs. D. Clauss and Mrs. Z.I. Apostolou-Keiper for preparation of the immunohistological slides.

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Authors and Affiliations

  1. Department of Neurosurgery, Johannes Wesling Klinikum, Hans-Nolte-Str. 1, 32429, Minden, Germany

    Ulrich J. Knappe

  2. Institute of Pathology and Cytology, Dr.-Horst-Schmidt-Klinik, Wiesbaden, Germany

    Thomas Fink & Annette Fisseler-Eckhoff

  3. Department of Neurosurgery, Dr.-Horst-Schmidt-Klinik, Wiesbaden, Germany

    Robert Schoenmayr

Authors
  1. Ulrich J. Knappe
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  2. Thomas Fink
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  3. Annette Fisseler-Eckhoff
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  4. Robert Schoenmayr
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Corresponding author

Correspondence to Ulrich J. Knappe.

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Comment

The authors present a cadaveric study of the extracellular matrix proteins in the perisellar area. The study indicates that the ECM at junction of the pituitary and the medial wall of the cavernous sinus is highly variable. Cavernous sinus invasion by pituitary adenomas may not simply relate to biological nature of the adenoma itself but also to anatomical differences between patients whose pituitary capsule and medial cavernous sinus walls may be more susceptible to penetration.

John Jane, Jr. M.D.,

Charlottesville, USA

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Knappe, U.J., Fink, T., Fisseler-Eckhoff, A. et al. Expression of extracellular matrix-proteins in perisellar connective tissue and dura mater. Acta Neurochir 152, 345–353 (2010). https://doi.org/10.1007/s00701-009-0497-9

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  • DOI: https://doi.org/10.1007/s00701-009-0497-9

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