Skip to main content
SpringerLink
Log in

Taxonomic novelties in Aspergillus section Fumigati: A. tasmanicus sp. nov., induction of sexual state in A. turcosus and overview of related species

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

The phylogenetic position of two Aspergillus strains isolated from Australian soil and phenotypically resembling A. unilateralis was investigated by using multigene phylogeny based on β-tubulin (benA), calmodulin (CaM), actin (act), and RNA polymerase II second largest subunit (RPB2) genes. The analysis supported their placement into a separate lineage within a well-supported clade containing 10 other members of section Fumigati (“A. unilateralis clade”). Comparisons of extrolite profiles, taxonomically informative morphological and physiological characters were made, and it was discovered that the two strains can be differentiated from all relatives by their low maximum growth temperature, short stipes, and ornamentation of conidia. The data justified the proposal of a new species, A. tasmanius sp. nov. Amplification of mating-type genes showed that the A. unilateralis clade contains five heterothallic species. Only the MAT1-1-1 idiomorph was detected among isolates of A. unilateralis, A. tasmanicus, and A. marvanovae, while isolates having both opposite mating types were detected in A. turcosus and A. nishimurae. The sexual state of A. turcosus was induced by mating experiments and is described in this study. Ascospores of this species were unique by their smooth to finely verrucose convex surface and two well-visible equatorial crests. Some exometabolites detected in A. marvanovae and A. tasmanicus are also indicative of a perfect state, thus supporting the hypothesis that these species have cryptic sexual cycles. The epitype and ex-epitype culture is designated for A. nishimurae to facilitate further taxonomic work with this species.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Alcazar-Fuoli L, Mellado E, Alastruey-Izquierdo A, Cuenca-Estrella M, Rodriguez-Tudela JL (2008) Aspergillus section Fumigati: antifungal susceptibility patterns and sequence-based identification. Antimicrob Agents Chemotherapy 52:1244–1251. doi:10.1128/AAC.00942-07

    Article  CAS  Google Scholar 

  • Anzawa K, Kawasaki M, Mochizuki T, Ishizaki H (2010) Successful mating of Trichophyton rubrum with Arthroderma simii. Med Mycol 48:629–634

    Article  CAS  PubMed  Google Scholar 

  • Balajee SA, Gribskov J, Brandt M, Ito J, Fothergill A, Marr KA (2005a) Mistaken identity: Neosartorya pseudofischeri and its anamorph masquerading as Aspergillus fumigatus. J Clin Microbiol 43:5996–5999. doi:10.1128/JCM.43.12.5996-5999.2005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Balajee SA, Gribskov JL, Hanley E, Nickle D, Marr KA (2005b) Aspergillus lentulus sp. nov., a new sibling species of A. fumigatus. Eukar Cell 4:625–632. doi:10.1128/EC.4.3.625-632.2005

    Article  CAS  Google Scholar 

  • Balajee SA, Nickle D, Varga J, Marr KA (2006) Molecular studies reveal frequent misidentification of Aspergillus fumigatus by morphotyping. Eukar Cell 5:1705–1712. doi:10.1128/EC.00162-06

    Article  CAS  Google Scholar 

  • Balajee SA, Kano R, Baddley JW, Moser SA, Marr KA, Alexander BD, Andes D, Kontoyiannis DP, Perrone G, Peterson S, Brandt ME, Pappas PG, Chiller T (2009) Molecular identification of Aspergillus species collected for the transplant-associated infection surveillance network. J Clin Microbiol 47:3138–3141. doi:10.1128/JCM.01070-09

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Barrs VR, van Doorn TM, Houbraken J, Kidd SE, Martin P, Pinheiro MD, Richardson M, Varga J, Samson RA (2013) Aspergillus felis sp. nov., an emerging agent of invasive aspergillosis in humans, cats and dogs. PLoS ONE 8:e64871. doi:10.1371/journal.pone.0064871

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Capon RJ, Ratnayake R, Stewart M, Lacey E, Tennant S, Gill JH (2005) Aspergillazines A–E: novel heterocyclic dipeptides from an Australian strain of Aspergillus unilateralis. Organic Biomolec Chem 3:123–129. doi:10.1039/B413440K

    Article  CAS  Google Scholar 

  • Carbone I, Kohn LM (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91:553–556. doi:10.2307/3761358

    Article  CAS  Google Scholar 

  • Chen A, Frisvad J, Sun B, Varga J, Kocsubé S, Dijksterhuis J, Kim D, Hong S-B, Houbraken J, Samson R (2016) Aspergillus section Nidulantes (formerly Emericella): polyphasic taxonomy, chemistry and biology. Stud Mycol 84:1–118. doi:10.1016/j.simyco.2016.10.001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Dethoup T, Gomes NGM, Chaopongpang S, Kijjoa A (2016) Aspergillus similanensis sp. nov. from a marine sponge in Thailand. Mycotaxon 131:7–15. doi:10.5248/131.7

    Article  Google Scholar 

  • Dyer PS, O’Gorman CM (2011) A fungal sexual revolution: Aspergillus and Penicillium show the way. Curr Opin Microbiol 14:649–654. doi:10.1016/j.mib.2011.10.001

    Article  PubMed  Google Scholar 

  • Frisvad JC, Larsen TO (2015a) Extrolites of Aspergillus fumigatus and other pathogenic species in Aspergillus section Fumigati. Frontiers Microbiol 6:1485. doi:10.3389/fmicb.2015.01485

    Google Scholar 

  • Frisvad JC, Larsen TO (2015b) Chemodiversity in the genus Aspergillus. Appl Microbiol Biotechnol 99:7859–7877. doi:10.1007/s00253-015-6839-z

    Article  CAS  PubMed  Google Scholar 

  • Frisvad JC, Thrane U (1987) Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV–Vis spectra (diode array detection). J Chromatogr A 404:195–214

    Article  CAS  Google Scholar 

  • Frisvad JC, Thrane U (1993) Liquid column chromatography of mycotoxins. In: Betina V (ed) Chromatography of mycotoxins: techniques and applications. J Chromatogr Libr 54:253–372. doi:10.1016/S0301-4770(08)60568-0

  • Frisvad JC, Rank C, Nielsen KF, Larsen TO (2009) Metabolomics of Aspergillus fumigatus. Med Mycol 47:S53–S71. doi:10.1080/13693780802307720

    Article  CAS  PubMed  Google Scholar 

  • Gautier M, Normand A-C, Ranque S (2016) Previously unknown species of Aspergillus. Clin Microbiol Infect 22:662–669. doi:10.1016/j.cmi.2016.05.013

    Article  CAS  PubMed  Google Scholar 

  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environm Microbiol 61:1323–1330

    CAS  Google Scholar 

  • Guarro J, Kallas EG, Godoy P, Karenina A, Gene J, Stchigel A, Colombo AL (2002) Cerebral aspergillosis caused by Neosartorya hiratsukae, Brazil. Emerging Infect Dis 8:989–992

    Article  PubMed  PubMed Central  Google Scholar 

  • Han JI, Na KJ (2008) Dermatitis caused by Neosartorya hiratsukae infection in a hedgehog. J Clin Microbiol 46:3119–3123. doi:10.1128/JCM.00737-08

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hearn V, Moutaouakil M, Latgé J-P (1990) Analysis of components of Aspergillus and Neosartorya mycelial preparations by gel electrophoresis and Western blotting procedures. In: Samson RA, Pitt JI (eds) Modern concepts in Penicillium and Aspergillus classification. Springer, Berlin, pp 235–247. doi:10.1007/978-1-4899-3579-3_20

    Chapter  Google Scholar 

  • Hong SB, Go SJ, Shin HD, Frisvad JC, Samson RA (2005) Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia 97:1316–1329

    Article  CAS  PubMed  Google Scholar 

  • Hong SB, Shin HD, Hong J, Frisvad JC, Nielsen PV, Varga J, Samson RA (2008) New taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati. Antonie van Leeuwenhoek J Microbiol Serol 93:87–98. doi:10.1007/s10482-007-9183-1

    Article  Google Scholar 

  • Hong S-B, Kim D-H, Park I-C, Samson RA, Shin H-D (2010) Isolation and identification of Aspergillus section Fumigati strains from arable soil in Korea. Mycobiology 38:1–6. doi:10.4489/MYCO.2010.38.1.001

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Houbraken J, Spierenburg H, Frisvad JC (2012) Rasamsonia, a new genus comprising thermotolerant and thermophilic Talaromyces and Geosmithia species. Antonie van Leeuwenhoek J Microbiol Serol 101:403–421. doi:10.1007/s10482-011-9647-1

    Article  CAS  Google Scholar 

  • Houbraken J, Weig M, Groß U, Meijer M, Bader O (2016) Aspergillus oerlinghausenensis, a new mould species closely related to A. fumigatus. FEMS Microbiol Lett 363:fnv236. doi:10.1093/femsle/fnv236

    Article  PubMed  Google Scholar 

  • Hubka V, Kolařík M (2012) β-tubulin paralogue tubC is frequently misidentified as the benA gene in Aspergillus section Nigri taxonomy: primer specificity testing and taxonomic consequences. Persoonia 29:1–10. doi:10.3767/003158512X658123

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Hubka V, Kubatova A, Mallatova N, Sedlacek P, Melichar J, Skorepova M, Mencl K, Lyskova P, Sramkova B, Chudickova M, Hamal P, Kolarik M (2012) Rare and new aetiological agents revealed among 178 clinical Aspergillus strains obtained from Czech patients and characterised by molecular sequencing. Med Mycol 50:601–610. doi:10.3109/13693786.2012.667578

    Article  CAS  PubMed  Google Scholar 

  • Hubka V, Peterson SW, Frisvad JC, Yaguchi T, Kubátová A, Kolařík M (2013) Aspergillus waksmanii sp. nov. and Aspergillus marvanovae sp. nov., two closely related species in section Fumigati. Int J Syst Evol Microbiol 63:783–789. doi:10.1099/ijs.0.047076-0

    Article  CAS  PubMed  Google Scholar 

  • Hubka V, Lyskova P, Frisvad JC, Peterson SW, Skorepova M, Kolarik M (2014) Aspergillus pragensis sp. nov. discovered during molecular re-identification of clinical isolates belonging to Aspergillus section Candidi. Med Mycol 52:565–576. doi:10.1093/mmy/myu022

    Article  CAS  PubMed  Google Scholar 

  • Hubka V, Nissen C, Jensen R, Arendrup M, Cmokova A, Kubatova A, Skorepova M (2015a) Discovery of a sexual stage in Trichophyton onychocola, a presumed geophilic dermatophyte isolated from toenails of patients with a history of T. rubrum onychomycosis. Med Mycol 53:798–809. doi:10.1093/mmy/myv044

    Article  PubMed  Google Scholar 

  • Hubka V, Nováková A, Kolařík M, Jurjević Ž, Peterson SW (2015b) Revision of Aspergillus section Flavipedes: seven new species and proposal of section Jani sect. nov. Mycologia 107:169–208. doi:10.3852/14-059

    Article  PubMed  Google Scholar 

  • Hubka V, Nováková A, Peterson SW, Frisvad JC, Sklenář F, Matsuzawa T, Kubátová A, Kolařík M (2016) A reappraisal of Aspergillus section Nidulantes with descriptions of two new sterigmatocystin producing species. Pl Syst Evol 302:1267–1299. doi:10.1007/s00606-016-1331-5

    Article  CAS  Google Scholar 

  • Jurjević Ž, Kubátová A, Kolařík M, Hubka V (2015) Taxonomy of Aspergillus section Petersonii sect. nov. encompassing indoor and soil-borne species with predominant tropical distribution. Pl Syst Evol 301:2441–2462. doi:10.1007/s00606-015-1248-4

    Article  Google Scholar 

  • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molec Biol Evol 30:772–780. doi:10.1093/molbev/mst010

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kocsubé S, Perrone G, Magistà D, Houbraken J, Varga J, Szigeti G, Hubka V, Hong S-B, Frisvad J, Samson R (2016) Aspergillus is monophyletic: evidence from multiple gene phylogenies and extrolites profiles. Stud Mycol 85:199–213. doi:10.1016/j.simyco.2016.11.006

    Article  PubMed  PubMed Central  Google Scholar 

  • Kornerup A, Wanscher JH (1967) Methuen handbook of colour. Methuen & Co., Ltd., London

    Google Scholar 

  • Koutroutsos K, Arabatzis M, Bougatsos G, Xanthaki A, Toutouza M, Velegraki A (2010) Neosartorya hiratsukae peritonitis through continuous ambulatory peritoneal dialysis. J Med Microbiol 59:862–865. doi:10.1099/jmm.0.019133-0

    Article  PubMed  Google Scholar 

  • Kretzer A, Li Y, Szaro T, Bruns TD (1996) Internal transcribed spacer sequences from 38 recognized species of Suillus sensu lato: phylogenetic and taxonomic implications. Mycologia 88:776–785. doi:10.2307/3760972

    Article  CAS  Google Scholar 

  • Kwon-Chung K, Kim SJ (1974) A second heterothallic Aspergillus. Mycologia 66:628–638. doi:10.2307/3758166

    Article  CAS  PubMed  Google Scholar 

  • Kwon-Chung KJ, Sugui JA (2009) Sexual reproduction in Aspergillus species of medical or economical importance: why so fastidious? Trends Microbiol 17:481–487. doi:10.1016/j.tim.2009.08.004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lanfear R, Calcott B, Ho SY, Guindon S (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molec Biol Evol 29:1695–1701. doi:10.1093/molbev/mss020

    Article  CAS  PubMed  Google Scholar 

  • Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molec Biol Evol 16:1799–1808. doi:10.1093/oxfordjournals.molbev.a026092

    Article  CAS  PubMed  Google Scholar 

  • Malejczyk K, Sigler L, Gibas CFC, Smith SW (2013) Invasive sino-orbital mycosis in an aplastic anemia patient caused by Neosartorya laciniosa. J Clin Microbiol 51:1316–1319. doi:10.1128/JCM.02919-12

    Article  PubMed  PubMed Central  Google Scholar 

  • Matsuzawa T, Campos Takaki GM, Yaguchi T, Okada K, Gonoi T, Horie Y (2013) Two new species of Aspergillus section Fumigati isolated from caatinga soil in the State of Pernambuco, Brazil. Mycoscience 55:79–88. doi:10.1016/j.myc.2013.04.001

    Article  Google Scholar 

  • Matsuzawa T, Takaki GMC, Yaguchi T, Okada K, Abliz P, Gonoi T, Horie Y (2015) Aspergillus arcoverdensis, a new species of Aspergillus section Fumigati isolated from caatinga soil in State of Pernambuco, Brazil. Mycoscience 56:123–131. doi:10.1016/j.myc.2014.04.006

    Article  Google Scholar 

  • Mayamor S, Poeaim S (2014) Morphological and molecular phylogeny studies on Eurotiales isolated from soil. J Agr Tech 10:189–195

    Google Scholar 

  • McLennan EI, Tucker SC, Thrower LB (1954) New soil fungi from Australian heathland: Aspergillus, Penicillium, Spegazzinia. Austral J Bot 2:355–364. doi:10.1071/BT9540355

    Google Scholar 

  • Meyer V, Wu B, Ram AF (2011) Aspergillus as a multi-purpose cell factory: current status and perspectives. Biotechnol Lett 33:469–476. doi:10.1007/s10529-010-0473-8

    Article  CAS  PubMed  Google Scholar 

  • Mirza J, Bajwa R (2005) New records of Fungi Imperfecti from Pakistan. Res Bull 1st Fung Cult Bank Pak 1:1–15

    Google Scholar 

  • Mühlenfeld A, Achenbacht H (1988) Asperpentyn, a novel acetylenic cyclohexene epoxide from Aspergillus duricaulis. Phytochemistry 27:3853–3855. doi:10.1016/0031-9422(88)83031-0

    Article  Google Scholar 

  • Nguyen L-T, Schmidt HA, von Haeseler A, Minh BQ (2015) IQ-TREE: a fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molec Biol Evol 32:268–274. doi:10.1093/molbev/msu300

    Article  CAS  PubMed  Google Scholar 

  • Nielsen KF, Månsson M, Rank C, Frisvad JC, Larsen TO (2011) Dereplication of microbial natural products by LC-DAD-TOFMS. J Nat Prod 74:2338–2348. doi:10.1021/np200254t

    Article  CAS  PubMed  Google Scholar 

  • Nováková A, Hubka V, Dudová Z, Matsuzawa T, Kubátová A, Yaguchi T, Kolařík M (2014) New species in Aspergillus section Fumigati from reclamation sites in Wyoming (USA) and revision of A. viridinutans complex. Fungal Diversity 64:253–274. doi:10.1007/s13225-013-0262-5

    Article  Google Scholar 

  • O’Gorman CM, Fuller HT, Dyer PS (2009) Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus. Nature 457:471–474. doi:10.1038/nature07528

    Article  PubMed  Google Scholar 

  • Olarte RA, Worthington CJ, Horn BW, Moore GG, Singh R, Monacell JT, Dorner JW, Stone EA, Xie DY, Carbone I (2015) Enhanced diversity and aflatoxigenicity in interspecific hybrids of Aspergillus flavus and Aspergillus parasiticus. Molec Ecol 24:1889–1909. doi:10.1111/mec.13153

    Article  CAS  Google Scholar 

  • Peterson SW (2008) Phylogenetic analysis of Aspergillus species using DNA sequences from four loci. Mycologia 100:205–226

    Article  CAS  PubMed  Google Scholar 

  • Pitt JI, Hocking AD (2009a) Aspergillus and related teleomorphs. In: Pitt JI, Hocking AD (eds) Fungi and food spoilage. Springer, London, pp 275–337. doi:10.1007/978-0-387-92207-2_8

    Chapter  Google Scholar 

  • Pitt JI, Hocking AD (2009b) Spoilage of stored, processed and preserved foods. In: Pitt JI, Hocking AD (eds) Fungi and food spoilage. Springer, London, pp 401–421. doi:10.1007/978-0-387-92207-2_12

    Chapter  Google Scholar 

  • Réblová M, Hubka V, Thureborn O, Lundberg J, Sallstedt T, Wedin M, Ivarsson M (2016) From the tunnels into the treetops: new lineages of black yeasts from biofilm in the Stockholm metro system and their relatives among ant-associated fungi in the Chaetothyriales. PLoS ONE 11:e0163396. doi:10.1371/journal.pone.0163396

    Article  PubMed  PubMed Central  Google Scholar 

  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol 61:539–542. doi:10.1093/sysbio/sys029

    Article  PubMed  PubMed Central  Google Scholar 

  • Salomão BC, Slongo AP, Aragão GM (2007) Heat resistance of Neosartorya fischeri in various juices. LWT Food Sci Technol 40:676–680. doi:10.1016/j.lwt.2006.03.008

    Article  Google Scholar 

  • Samson RA, Hong S, Peterson SW, Frisvad JC, Varga J (2007) Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya. Stud Mycol 59:147–203. doi:10.3114/sim.2007.59.14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Samson RA, Houbraken J, Thrane U, Frisvad JC, Andersen B (2010) Food and indoor fungi. CBS KNAW Biodiversity Center, Utrecht

    Google Scholar 

  • Samson RA, Visagie CM, Houbraken J, Hong SB, Hubka V, Klaassen CHV, Perrone G, Seifert KA, Tanney JB, Varga J, Yaguchi T, Frisvad JC (2014) Phylogeny, identification and nomenclature of the genus Aspergillus. Stud Mycol 78:141–173. doi:10.1016/j.simyco.2014.07.004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Santoianni J, Predari S, Verón D, Zucchini A, De Paulis A (2008) A 15 year-review of peritoneal dialysis-related peritonitis: microbiological trends and patterns of infection in a teaching hospital in Argentina. Revista Argent Microbiol 40:17–23

    CAS  Google Scholar 

  • Sheng-Liang Z, Shu-Zhen Y, Zhen-Ying W, Shuang-Lin C (2014) Endophytic fungi associated with Macrosolen tricolor and its host Camellia oleifera. World J Microbiol Biotechnol 30:1775–1784. doi:10.1007/s11274-014-1600-9

    Article  PubMed  Google Scholar 

  • Shivaprakash MR, Jain N, Gupta S, Baghela A, Gupta A, Chakrabarti A (2009) Allergic fungal rhinosinusitis caused by Neosartorya hiratsukae from India. Med Mycol 47:317–320. doi:10.1080/13693780802562977

    Article  CAS  PubMed  Google Scholar 

  • Sugui JA, Vinh DC, Nardone G, Shea YR, Chang YC, Zelazny AM, Marr KA, Holland SM, Kwon-Chung KJ (2010) Neosartorya udagawae (Aspergillus udagawae), an emerging agent of aspergillosis: how different is it from Aspergillus fumigatus? J Clin Microbiol 48:220–228. doi:10.1128/JCM.01556-09

    Article  CAS  PubMed  Google Scholar 

  • Sugui J, Peterson S, Figat A, Hansen B, Samson R, Mellado E, Cuenca-Estrella M, Kwon-Chung K (2014) Genetic relatedness versus biological compatibility between Aspergillus fumigatus and related species. J Clin Microbiol 52:3707–3721. doi:10.1128/JCM.01704-14

    Article  PubMed  PubMed Central  Google Scholar 

  • Sutton D et al (2002) First report of Neosartorya spinosa (Raper & Fennell) Kozakiewicz inciting human disease. American Society for Microbiology General Meeting, Salt Lake City, USA

  • Swilaiman SS, O’Gorman CM, Balajee SA, Dyer PS (2013) Discovery of a sexual cycle in Aspergillus lentulus, a close relative of A. fumigatus. Eukar Cell 12:962–969. doi:10.1128/EC.00040-13

    Article  CAS  Google Scholar 

  • Szewczyk E, Krappmann S (2010) Conserved regulators of mating are essential for Aspergillus fumigatus cleistothecium formation. Eukar Cell 9:774–783. doi:10.1128/EC.00375-09

    Article  CAS  Google Scholar 

  • Takada M, Horie Y, Abliz P (2001) Two new heterothallic Neosartorya from African soil. Mycoscience 42:361–367. doi:10.1007/BF02461219

    Article  Google Scholar 

  • Udagawa S, Tsubouchi H, Horie Y (1991) Neosartorya hiratsukae, a new species of food-borne Ascomycetes. Trans Mycol Soc Japan 32:23–29

    Google Scholar 

  • Verdejo-Lucas S, Blanco M, Talavera M, Stchigel A, Sorribas F (2013) Fungi recovered from root-knot nematodes infecting vegetables under protected cultivation. Biocontrol Sci Technol 23:277–287. doi:10.1080/09583157.2012.756459

    Article  Google Scholar 

  • Visagie CM, Hirooka Y, Tanney JB, Whitfield E, Mwange K, Meijer M, Amend AS, Seifert KA, Samson RA (2014) Aspergillus, Penicillium and Talaromyces isolated from house dust samples collected around the world. Stud Mycol 78:63–139. doi:10.1016/j.simyco.2014.07.002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang Q, Gong J, Chisti Y, Sirisansaneeyakul S (2015) Fungal isolates from a pu-erh type tea fermentation and their ability to convert tea polyphenols to theabrownins. J Food Sci 80:M809–M817. doi:10.1111/1750-3841.12831

    Article  CAS  PubMed  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA et al (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Yaguchi T, Someya A, Si Udagawa (1994) A new species of Neosartorya from Taiwan soil. Mycoscience 35:309–313. doi:10.1007/BF02268499

    Article  Google Scholar 

  • Yaguchi T, Horie Y, Tanaka R, Matsuzawa T, Ito J, Nishimura K (2007) Molecular phylogenetics of multiple genes on Aspergillus section Fumigati isolated from clinical specimens in Japan. Jap J Med Mycol 48:37–46. doi:10.3314/jjmm.48.37

    Article  CAS  Google Scholar 

  • Yaguchi T, Matsuzawa T, Tanaka R, Abliz P, Hui Y, Horie Y (2010) Two new species of Neosartorya isolated from soil in Xinjiang, China. Mycoscience 51:253–262. doi:10.1007/s10267-010-0037-8

    Article  Google Scholar 

Download references

Acknowledgements

This research was supported by the project of Charles University Grant Agency (GAUK 1130214), and the project BIOCEV (CZ.1.05/1.1.00/02.0109) provided by the Ministry of Education, Youth and Sports of CR and ERDF. Prof Karol Marhold’s consultation on nomenclatural issues is gratefully acknowledged. We thank Milada Chudíčková, Alena Gabrielová and Kyung-youn Hong for their invaluable assistance in laboratory and Veronica Jurjević for assistance with editing.

Author information

Authors and Affiliations

  1. Department of Botany, Faculty of Science, Charles University, Benátská 2, 128 01, Prague 2, Czech Republic

    Vit Hubka, Zuzana Dudová, Alena Kubátová & Miroslav Kolařík

  2. Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology AS CR, v.v.i, Vídeňská 1083, 142 20, Prague 4, Czech Republic

    Vit Hubka & Miroslav Kolařík

  3. First Faculty of Medicine, Charles University, Kateřinská 32, 121 08, Prague 2, Czech Republic

    Vit Hubka

  4. Department of Systems Biology, Building 221, Center for Microbial Biotechnology, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark

    Jens C. Frisvad

  5. Medical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8673, Japan

    Takashi Yaguchi & Yoshikazu Horie

  6. EMSL Analytical, Inc., 200 Route 130 North, Cinnaminson, NJ, 08077, USA

    Željko Jurjević

  7. Korean Agricultural Culture Collection, National Institute of Agricultural Science, 166, Nongsaengmyeong-ro, Iseo-myeon, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea

    Seung-Beom Hong

Authors
  1. Vit Hubka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zuzana Dudová
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alena Kubátová
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jens C. Frisvad
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Takashi Yaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Yoshikazu Horie
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Željko Jurjević
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Seung-Beom Hong
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Miroslav Kolařík
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Vit Hubka.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicts of interest.

Human and animal rights

This article does not contain any studies with human participants or animals performed by any of the authors.

Additional information

Handling editor: Stephen W. Peterson.

Information on Electronic Supplementary Material

Below is the link to the electronic supplementary material.

Online Resource 1

Supplementary material 1 (DOC 47 kb)

Online Resource 2

Supplementary material 2 (PDF 21 kb)

Information on Electronic Supplementary Material

Information on Electronic Supplementary Material

Online Resource 1. A concatenated alignment of β-tubulin (benA), calmodulin (CaM), actin (act) and RNA polymerase II second largest subunit (RPB2) genes used for construction of tree on Fig. 1: benA: positions 1–537; CaM: positions 538–1231; act: positions 1232–1669; RPB2: positions 1670–2667.

Online Resource 2. Secondary metabolites detected in species from Aspergillus unilateralis clade and their absorption maxima, and bracketed retention indexes (RI) based on a series of alkylphenones (Frisvad and Thrane 1987, 1993).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hubka, V., Dudová, Z., Kubátová, A. et al. Taxonomic novelties in Aspergillus section Fumigati: A. tasmanicus sp. nov., induction of sexual state in A. turcosus and overview of related species. Plant Syst Evol 303, 787–806 (2017). https://doi.org/10.1007/s00606-017-1400-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-017-1400-4

Keywords

Search

Navigation