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Micromorphology and anatomy of the leaf blade: a contribution to the taxonomy of Luziola (Poaceae, Oryzoideae) from the Pantanal, Brazil

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Abstract

Luziola comprises nine monoecious, aquatic species and has its center of diversity in South America. Due to the morphological similarities among Luziola species in the vegetative stage, the micromorphology and anatomy of the leaf blade of five Luziola species were studied to survey potentially useful features for taxon identification. Oryzoid silica bodies; silicified unicellular trichomes with a rounded apex; stomata in furrows on the adaxial surface; a complex midrib consisting of at least two vascular bundles; superposed vascular bundles in the mesophyll; and fusoid cells are features shared by some of these species. Conversely, features unique to a single species include: vertically elongated epidermal cells surrounding pointed unicellular trichomes, flat midrib and leaf margin dimorphism (L. fragilis), and an obtuse leaf blade margin (L. subintegra). L. spruceana and L. subintegra show the greatest micromorphological and anatomical affinity among the five species herein studied, and L. fragilis has the greatest number of unique features. All species are recognized by the structure of the midrib, a strong feature for the identification of taxa within Luziola. A key for the identification of the studied species is presented based on the leaf blade features.

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Acknowledgments

The authors are grateful to the curators of the CGMS Herbarium/UFMS for the donation of material; V Manvailer for assistance with image processing; LF Plaça for assistance with generating SEM micrographs, captured at the Multiuser Laboratory of Scanning Electron Microscopy/UFMS. The authors thank PNADB/CAPES and CNPq (process numbers: 308793/2013-7 and 311267/2012-2) for financial support. We also thank an anonymous referee and TJ Gallaher for comments that greatly improved the manuscript.

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Authors and Affiliations

  1. Universidade Estadual Paulista, Av. 24A 1515, Bela Vista, Rio Claro, SP, 13506-900, Brazil

    Thales D. Leandro

  2. Universidade Federal de Mato Grosso do Sul, Av. Costa e Silva s/nº, Caixa Postal 549, Cidade Universitária, Campo Grande, MS, 79070-900, Brazil

    Edna Scremin-Dias & Rosani do Carmo de Oliveira Arruda

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  1. Thales D. Leandro
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  2. Edna Scremin-Dias
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Correspondence to Thales D. Leandro.

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Handling editor: Karol Marhold.

Part of Master’s Thesis research of the first author, Programa de Pós-Graduação em Biologia Vegetal, UFMS, Brazil.

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Leandro, T.D., Scremin-Dias, E. & do Carmo de Oliveira Arruda, R. Micromorphology and anatomy of the leaf blade: a contribution to the taxonomy of Luziola (Poaceae, Oryzoideae) from the Pantanal, Brazil. Plant Syst Evol 302, 265–273 (2016). https://doi.org/10.1007/s00606-015-1260-8

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  • DOI: https://doi.org/10.1007/s00606-015-1260-8

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