Skip to main content
SpringerLink
Log in

Genetic diversity and relationships between and within persimmon (Diospyros L.) wild species and cultivated varieties by SRAP markers

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Persimmon (Diospyros L.) is one of the most important fruits worldwide and it has been recognized as a health nutrient supply for human consumption. In this study, sequence-related amplified polymorphism (SRAP) markers were used to characterize the genetic diversity of seven species of Persimmon, aiming at exploiting valuable wild resources for breeding new cultivars. Our results show that 303 out of a total of 303 bands amplified with 11 SRAP primer combinations reveal highly polymorphism. The 48 genotypes of persimmons were divided into five groups using the unweighted pair-group method of arithmetic average cluster analysis and principal coordinated analysis. The clusters results show that not only interspecies among seven persimmon genus, but also inner-species of each genus could be clearly differentiated. The phylogenetics positions of Deyang yeshi, Yemaoshi and Jinzaoshi have been elucidated. The results also indicated that wild Chinese persimmon species bear diverse genetic makeup.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Badenes ML, Garcés A, Romero C, Romero M, Clavé J, Rovira M, Llácer G (2003) Genetic diversity of introduced and local Spanish persimmon cultivars revealed by RAPD markers. Genet Res Crop Evol 50:579–585

    Article  CAS  Google Scholar 

  • Budak H, Shearman RC, Parmaksiz I, Dweikat I (2004) Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs. Theor Appl Genet 109:280–288

    Article  PubMed  CAS  Google Scholar 

  • Choi YA, Tao R, Yonemori K, Sugiura A (2003) Genomic distribution of three repetitive DNAs in cultivated hexaploid Diospyros spp. (D. kaki and D. virginiana) and their wild relatives. Genes Genet Syst 78:301–308

    Article  PubMed  CAS  Google Scholar 

  • Dong P, Wei YM, Chen GY, Li W, Wang JR, Nevo E, Zheng YL (2010) Sequence-related amplified polymorphism (SRAP) of wild emmer wheat (Triticum dicoccoides) in Israel and its ecological association. Biochem Syst Ecol 38:1–11

    Article  CAS  Google Scholar 

  • Doyle JJ, Doyle JL (1987) A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Du XY, Zhang QL, Luo ZR (2009) Development of retrotransposon primers and their utilization for germplasm identification in Diospyros spp. (Ebenaceae). Tree Genet Genom 5:235–245

    Article  Google Scholar 

  • Ferriol M, Pico B, Nuez F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor Appl Genet 107:271–282

    Article  PubMed  CAS  Google Scholar 

  • Greene SL, Morris JB (2001) The case for multiple-use plant germplasm collections and a strategy for implementation. Crop Sci 41(3):886–892

    Article  Google Scholar 

  • Guo DL, Luo ZR (2006) Genetic relationships of some PCNA persimmons (Diospyros kaki Thunb.) from China and Japan revealed by SRAP analysis. Genet Res Crop Evol 53:1597–1603

    Article  CAS  Google Scholar 

  • Guo DL, Zhang HQ, Luo ZR (2006) Genetic relationships of Diospyros kaki Thunb. and related species revealed by IRAP and REMAP analysis. Plant Sci 170:528–533

    Article  CAS  Google Scholar 

  • Hu DC, Luo ZR (2006) Polymorphisms of amplified mitochondrial DNA non-coding regions in Diospyros spp. Sci Hortic 109:275–281

    Article  CAS  Google Scholar 

  • Jing ZB, Wang XP (2013) Genetic relationship between Chinese wild Vitis species and American and European Cultivars based on ISSR markers. Biochem Syst Ecol 46:120–126

    Article  CAS  Google Scholar 

  • Kanzaki S, Yonemori K, Sato A, Yamada M, Sugiura A (2000a) Evaluation of RFLP analysis for discriminating PCNA genotype in some persimmon cultivars. J Jpn Soc Hort Sci 69:702–704

    Article  CAS  Google Scholar 

  • Kanzaki S, Yonemori K, Sato A, Yamada M, Sugiura A (2000b) Analysis of the genetic relationships among pollination-constant and non-astringent (PCNA) cultivars of persimmon (Diospyros kaki Thunb.) from Japan and China using amplified fragment length polymorphism (AFLP). J Jpn Soc Hort Sci 69:665–670

    Article  CAS  Google Scholar 

  • Li G, Quiros CF (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103(2–3):455–461

    Article  CAS  Google Scholar 

  • Luo ZR, Yonemori K, Sugiura A (1995) Evaluation of RAPD analysis for cultivar identification of persimmons. J Jpn Soc Hort Sci 64:535–541

    Article  CAS  Google Scholar 

  • Maki S, Oyama K, Kurahashi T, Nakahira T, Kawabata T, Yamada T (2001) RFLP analysis for cultivar identification of persimmons. Sci Hortic 91:407–412

    Article  CAS  Google Scholar 

  • Nakamura Y, Kobayashi S (1994) DNA restriction fragment length variability in Diospyros kaki and related Diospyros species. Hort Sci 29:809–811

    CAS  Google Scholar 

  • Nakasuka A, Iwami N, Matusmoto S, Itamura H, Yamagishi M (2002) Ty1-copia group retrotransposons in persimmon (Diospyros kaki Thunb.). Genes Genet Syst 77:131–136

    Article  Google Scholar 

  • Naval MD, Zuriaga E, Llácer G, Pecchioli S, Giordani E, Badenes ML (2010) Analysis of genetic diversity among persimmon cultivars using microsatellite markers. Tree Genet Genomes 6:677–687

    Article  Google Scholar 

  • Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 19:583–590

    Google Scholar 

  • Ng FSP (1978) Diospyros roxburghii and the origin of Dio-spyros kaki. Malay For 41:43–50

    Google Scholar 

  • Rohlf FJ (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system. Version 2.1. Exeter Software, Setauket

    Google Scholar 

  • Yamagishi M, Matsumoto S, Nakatsuka A, Itamura H (2005) Identification of persimmon (Diospyros kaki) cultivars and phenetic relationships between Diospyros species by more effective RAPD analysis. Sci Hortic 105:283–290

    Article  CAS  Google Scholar 

  • Yonemori K, Kanzaki S, Parfitt DE, Utsunomiya N, Subhadrabandhu S, Sugiura A (1998) Phylogenetic relationship of Diospyros kaki (persimmon) to Diospyros spp. (Ebenaceae) of Thailand and four temperate zone Diospyros spp. from an analysis of RFLP variation in amplified cpDNA. Genome 41:173–182

    CAS  Google Scholar 

  • Yonemori K, Sugiura A, Yamada M (2000) Persimmon genetics and breeding. In: Janick C (ed) Plant breeding reviews, vol 19. Wiley, New York, pp 191–225

    Google Scholar 

  • Yonemori K, Honsho C, Kitajima A, Aradhya M, Giordani E, Bellini E, Parfitt DE (2008a) Relationship of European persimmon (Diospyros kaki Thunb.) cultivars to Asian cultivars, characterized using AFLPs. Gen Res Crop Evol 55:81–89

    Article  CAS  Google Scholar 

  • Yonemori K, Kanzaki S, Honsho C, Akagi T, Parfitt DE (2008b) Phylogeny and cultivar development of Diospyrus kaki: a survey based on molecular analysis. Adv Hort Sci 22(4):261–268

    Google Scholar 

  • Yonemori Y, Yamada M, Luo ZR, Kanzaki S, Yang Y, Wang RZ (2012) New candidate for the ancestor of Japanese persimmon (Diospyros kaki Thunb.) found in Yunnan province of China. Acta Hort 948:121–128

    Google Scholar 

Download references

Acknowledgments

The authors wish to thank Prof. Xiping Wang for his valuable suggestions and Jianping Wang for samples collecting. This work was financially supported by “Project for Crop Germplasm Conservation” grant from China Agriculture Ministry (NB2012-2130135-21) and Special Fund for Agro-scientific Research in the Public Interest (201203047).

Author information

Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Zhaobin Jing

  2. College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Zhaobin Jing, Xiaofeng Ruan, Renzi Wang & Yong Yang

Authors
  1. Zhaobin Jing
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaofeng Ruan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Renzi Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yong Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yong Yang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jing, Z., Ruan, X., Wang, R. et al. Genetic diversity and relationships between and within persimmon (Diospyros L.) wild species and cultivated varieties by SRAP markers. Plant Syst Evol 299, 1485–1492 (2013). https://doi.org/10.1007/s00606-013-0810-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-013-0810-1

Keywords

Search

Navigation