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Karyotypic, palynological, and RAPD study on 12 taxa from two subsections of section Idaeobatus in Rubus L. and taxonomic treatment of R. ellipticus, R. pinfaensis, and R. ellipticus var. obcordatus

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Abstract

Twelve taxa belonging to two subsections of section Idaeobatus in Rubus L. from southwestern China were characterized by karyotypic, palynological, and random amplified polymorphic DNA (RAPD) data as follows: (1) The 12 taxa were all diploid species (2n = 2x = 14), among which the chromosome counts for R. mesogaeus var. oxycomus, R. subtibetanus, R. ellipticus var. obcordatus, R. inopertus var. echinocalyx, and R. stans were reported for the first time; (2) All taxa except for R. ellipticus and R. pinfaensis could be distinguished from each other by karyotype, pollen morphology, and RAPD markers. Karyotypes were mainly characterized by the difference in numbers and positions of submetacentric chromosomes and chromosomes with satellited pair, the index of the karyotypic asymmetry, and the ratio of the longest to the shortest chromosome. Pollen morphology were mainly characterized by the discrepancy in specific pollen size, P/E ratio, colpi width, distance between the apices of two ectocolpi, and exine ornamentation characters. The cluster results based on RAPD markers were consistent with morphology classification except for R. pinfaensis; (3) Based on the general data of karyotypic, palynological, and RAPD, R. ellipticus var. obcordatus should be treated as a species R. obcordatus, R. ellipticus and R. pinfaensis should be combined as R. ellipticus, and it was more reasonable to place the combinants and R. obcordatus into subsection Stimulantes rather than into subsection Pungentes.

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Acknowledgments

The authors are thankful to the Program for the National Natural Science Foundation of China (30671454) and the Education Bureau of Sichuan Province, China for financial support (2004A025). We particularly thank Prof. Guang-hui Yang (Sichuan Agricultural University) for her help in the course of specimen identification.

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  1. College of Horticulture, Sichuan Agricultural University, Xinkang Road 46#, 625014, Ya’an, Sichuan, China

    Xiao-Rong Wang, Hao-Ru Tang, Han-Wu Zhang, Bi-Feng Zhong, Wu-Feng Xia & Yuan Liu

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  1. Xiao-Rong Wang
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  2. Hao-Ru Tang
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  4. Bi-Feng Zhong
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Correspondence to Hao-Ru Tang.

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Wang, XR., Tang, HR., Zhang, HW. et al. Karyotypic, palynological, and RAPD study on 12 taxa from two subsections of section Idaeobatus in Rubus L. and taxonomic treatment of R. ellipticus, R. pinfaensis, and R. ellipticus var. obcordatus . Plant Syst Evol 283, 9–18 (2009). https://doi.org/10.1007/s00606-009-0190-8

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