Skip to main content
SpringerLink
Log in

Evidence for multiple, autoploid origins of agamospermous populations in Eupatorium sessilifolium (Asteraceae)

  • Original Article
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

Comparative analyses were made of agamospermous populations of Eupatorium sessilifolium, which have previously been documented to be polyploid, to determine whether they are alloploid or autoploid in origin and to assess the possibility that they have arisen more than once. There was no variability in ITS sequences among seven agamospermous and eight sexual diploid populations of E. sessilifolium, which is consistent with morphological observations in suggesting that the agamospermous populations were autoploids. The ITS sequence characteristic of E. sessilifolium differs from all other North American species by a minimum of 15 changes, and heterogeneity or polymorphism would be expected if the agamospermous populations were alloploids. Analysis of the chloroplast-based trnC-psbM spacer region showed variability among both sexual diploid and agamospermous populations of E. sessilifolium, which suggested that the agamospermous populations stem from multiple origins. Analysis of ISSR data revealed considerable intraspecific variability within E. sessilifolium, and the distribution of variability, with agamospermous populations showing variability from one another, added further evidence for multiple origins of agamospermous populations. The results in conjunction with distributional evidence that the sexual diploid populations of E. sessilifolium are geographically restricted and uncommon suggest that monitoring of populations might be warranted to evaluate whether measures are needed to enhance their continued survival.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11–15

    Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Flagel LE, Rapp RE, Grover CE, Widrlechner MP, Hawkins J, Grafenberg JL, Álvarez I, Chung GY, Wendel JF (2008) Phylogenetic, morphological, and chemotaxonomic incongruence in the North American endemic genus Echinacea. Amer J Bot 95:756–765

    Article  CAS  Google Scholar 

  • Grant WF (1953) A cytotaxonomic study in the genus Eupatorium. Amer J Bot 40:729–742

    Article  Google Scholar 

  • Grant V (1981) Plant speciation, 2nd edn. Columbia University Press, New York

    Google Scholar 

  • Hollingsworth ML, Bailey JP (2000) Evidence for massive clonal growth in the invasive weed Fallopia japonica (Japanese knotweed). Bot J Linn Soc 133:463–472

    Google Scholar 

  • Montgomery JD, Fairbrothers DE (1970) A biosystematic study of the Eupatorium rotundifolium complex (Compositae). Brittonia 22:134–150

    Article  Google Scholar 

  • Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76:5269–5273

    Article  PubMed  CAS  Google Scholar 

  • Poulin J, Weller SG, Sakai AK (2005) Genetic diversity does not affect the invasiveness of fountain grass (Pennisetum setaceum) in Arizona, California and Hawaii. Divers Distrib 11:241–247

    Article  Google Scholar 

  • Reddy MP, Sarla N, Siddiq EA (2002) Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17

    Article  Google Scholar 

  • Schilling EE, LeBlond RJ, Sorrie BA, Weakley AS (2007) The relationships of the New England Boneset, Eupatorium novae-angliae (Asteraceae). Rhodora 109:145–160

    Article  Google Scholar 

  • Schmidt GJ, Schilling EE (2000) Phylogeny and biogeography of Eupatorium (Asteraceae: Eupatorieae) based on nuclear ITS sequence data. Amer J Bot 87:716–726

    Article  CAS  Google Scholar 

  • Shaw J, Lickey EB, Beck JT, Farmer SB, Liu W, Miller J, Siripun KC, Winder CT, Schilling EE, Small RL (2005) The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis. Amer J Bot 92:142–166

    Article  CAS  Google Scholar 

  • Siripun KC, Schilling EE (2006a) Molecular confirmation of the hybrid origin of Eupatorium godfreyanum (Asteraceae). Amer J Bot 93:319–325

    Article  CAS  Google Scholar 

  • Siripun KC, Schilling EE (2006b) Eupatorium, in Flora North America, Vol. 21: pp 462–474

  • Soltis DE, Soltis PS, Tate JA (2003) Advances in the study of polyploidy since Plant speciation. New Phytol 161:173–191

    Article  Google Scholar 

  • Soltis DE, Morris AB, McLachlan JS, Manos PS, Soltis PS (2006) Comparative phylogeography of unglaciated eastern North America. Molec Ecol 15:4261–4293

    Article  Google Scholar 

  • Soltis DE, Soltis PS, Schemske DW, Hancock JF, Thompson JN, Husband BC, Judd WS (2007) Autopolyploidy in angiosperms: have we grossly underestimated the number of species? Taxon 56:13–30

    Google Scholar 

  • Sullivan VI (1972) Investigation of the breeding systems, formation of auto- and alloploids and the reticulate pattern of hybridization in North American Eupatorium (Compositae). PhD dissertation, Florida State University, Tallahassee/University Microfilms, Ann Arbor

  • Sullivan VI (1976) Diploidy, polyploidy, and agamospermy among species of Eupatorium (Compositae). Canad J Bot 54:2907–2917

    Article  Google Scholar 

  • Sullivan VI, Neigel J, Miao B (1991) Bias in inheritance of chloroplast DNA and mechanisms of hybridization between wind-pollinated and insect-pollinated Eupatorium (Asteraceae). Amer J Bot 78:695–705

    Article  Google Scholar 

  • Swofford DL (2003) PAUP*. Phylogenetic analysis using parsimony (* and other methods). Version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • van Dijk PJ, Vijverberg K (2005) The significance of apomixis in the evolution of the angiosperms: a reappraisal. In: Bakker FT, Charton LW, Gravendeel B, Pelser PB (eds) Plant species-level systematics: new perspectives on pattern & process. Koeltz Scientific Books, Koenigstein, pp 101–116

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp 315–322

    Google Scholar 

  • Wolfe AD (2005) ISSR techniques for evolutionary biology. Meth Enzymol 395:134–144

    Article  PubMed  CAS  Google Scholar 

  • Wolfe AD, Liston A (1998) Contributions of PCR-based methods to plant systematics and evolution biology. In: Soltis DE, Soltis PS, Doyle JJ (eds) Molecular systematics of plants II. Kluwer, New York, pp 43–86

    Google Scholar 

  • Xu C-Y, Zhang W-J, Fu C-Z, Lu B-R (2003) Genetic diversity of alligator weed in China by RAPD analysis. Biodivers Conserv 12:637–645

    Article  Google Scholar 

  • Yahara T, Sullivan VI (1986) Peroxidase phenotypes of Eupatorium mohrii and E. scabridum (Compositae), widespread all-agamospermous “species” in the southeastern United States. Pl Spec Biol 1:27–34

    Article  Google Scholar 

  • Yahara T, Ito M, Watanabe K, Crawford DJ (1991) Very low genetic heterozygosities in sexual and agamospermous populations of Eupatorium altissimum (Asteraceae). Amer J Bot 78:706–710

    Article  Google Scholar 

Download references

Acknowledgments

The authors are grateful to G. Beattie, S. Robertson, and D. E. Schilling for technical help. Financial support was provided by grants from the US National Science Foundation (DEB-0343684) and the Hesler Fund of the University of Tennessee.

Author information

Authors and Affiliations

  1. Department of Science, Kasetsart University, Kamphangsaen Campus, Kamphangsaen, Nakhon Pathom, 73140, Thailand

    Kunsiri C. Grubbs

  2. Department of Ecology and Evolutionary Biology, University of Tennessee, Knoxville, TN, 37996, USA

    Randall L. Small & Edward E. Schilling

Authors
  1. Kunsiri C. Grubbs
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Randall L. Small
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Edward E. Schilling
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Edward E. Schilling.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Grubbs, K.C., Small, R.L. & Schilling, E.E. Evidence for multiple, autoploid origins of agamospermous populations in Eupatorium sessilifolium (Asteraceae). Plant Syst Evol 279, 151–161 (2009). https://doi.org/10.1007/s00606-009-0155-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00606-009-0155-y

Keywords

Search

Navigation